Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0949 and Psyr_0950 are separated by 0 nucleotides Psyr_0950 and Psyr_0951 overlap by 16 nucleotides Psyr_0951 and Psyr_0952 overlap by 8 nucleotides Psyr_0952 and Psyr_0953 are separated by 118 nucleotides
Psyr_0949: Psyr_0949 - bacterial peptide chain release factor 1 (bRF-1), at 1,084,901 to 1,085,983
_0949
Psyr_0950: Psyr_0950 - [protein release factor]-glutamine N5-methyltransferase, at 1,085,984 to 1,086,817
_0950
Psyr_0951: Psyr_0951 - UBA/THIF-type NAD/FAD binding fold:MoeZ/MoeB, at 1,086,802 to 1,087,581
_0951
Psyr_0952: Psyr_0952 - glutamate racemase, at 1,087,574 to 1,088,389
_0952
Psyr_0953: Psyr_0953 - conserved hypothetical protein, at 1,088,508 to 1,089,026
_0953
Position (kb)
1086
1087
1088 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1085.948 kb on + strand at 1085.981 kb on + strand at 1085.981 kb on + strand at 1086.003 kb on + strand at 1086.838 kb on + strand at 1086.884 kb on + strand, within Psyr_0951 at 1086.884 kb on + strand, within Psyr_0951 at 1086.949 kb on + strand, within Psyr_0951 at 1087.064 kb on + strand, within Psyr_0951 at 1087.478 kb on + strand, within Psyr_0951 at 1087.478 kb on + strand, within Psyr_0951 at 1087.588 kb on + strand at 1087.588 kb on + strand at 1087.588 kb on + strand at 1087.588 kb on + strand at 1088.317 kb on - strand at 1088.424 kb on + strand at 1088.424 kb on + strand at 1088.425 kb on - strand at 1088.425 kb on - strand at 1088.425 kb on - strand at 1088.445 kb on + strand at 1088.445 kb on + strand at 1088.446 kb on - strand at 1088.446 kb on - strand at 1088.446 kb on - strand at 1088.446 kb on - strand at 1088.446 kb on - strand at 1088.447 kb on + strand at 1088.447 kb on + strand at 1088.447 kb on + strand at 1088.486 kb on + strand at 1088.491 kb on + strand at 1088.491 kb on + strand at 1088.491 kb on + strand at 1088.491 kb on + strand at 1088.492 kb on - strand at 1088.492 kb on - strand at 1088.492 kb on - strand at 1088.492 kb on - strand at 1088.495 kb on + strand at 1088.496 kb on - strand at 1088.496 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 1,085,948 + -0.2 1,085,981 + -0.4 1,085,981 + +0.5 1,086,003 + -0.1 1,086,838 + -2.6 1,086,884 + Psyr_0951 0.11 -3.1 1,086,884 + Psyr_0951 0.11 -2.9 1,086,949 + Psyr_0951 0.19 -2.3 1,087,064 + Psyr_0951 0.34 -2.3 1,087,478 + Psyr_0951 0.87 -1.2 1,087,478 + Psyr_0951 0.87 -3.2 1,087,588 + -0.1 1,087,588 + +0.9 1,087,588 + +0.2 1,087,588 + +0.4 1,088,317 - -1.6 1,088,424 + +0.4 1,088,424 + +0.4 1,088,425 - -0.0 1,088,425 - -1.5 1,088,425 - -3.0 1,088,445 + +0.7 1,088,445 + +1.3 1,088,446 - -0.2 1,088,446 - -1.5 1,088,446 - -0.3 1,088,446 - +0.1 1,088,446 - -0.0 1,088,447 + +0.5 1,088,447 + -0.2 1,088,447 + -0.3 1,088,486 + -0.2 1,088,491 + -1.2 1,088,491 + +0.0 1,088,491 + -0.9 1,088,491 + -0.3 1,088,492 - +0.0 1,088,492 - -1.6 1,088,492 - +0.9 1,088,492 - +0.1 1,088,495 + +0.0 1,088,496 - +0.0 1,088,496 - -0.3
Or see this region's nucleotide sequence