Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0529 and Psyr_0530 overlap by 1 nucleotides Psyr_0530 and Psyr_0531 overlap by 4 nucleotides Psyr_0531 and Psyr_0532 are separated by 30 nucleotides
Psyr_0529: Psyr_0529 - Glycosyl transferase, group 1, at 597,719 to 598,849
_0529
Psyr_0530: Psyr_0530 - Mig-14, at 598,849 to 599,745
_0530
Psyr_0531: Psyr_0531 - LmbE-like protein, at 599,742 to 601,151
_0531
Psyr_0532: Psyr_0532 - conserved hypothetical protein, at 601,182 to 602,138
_0532
Position (kb) 599
600
601
602 Strain fitness (log2 ratio) -2
-1
0
1
2 at 598.799 kb on - strand at 599.218 kb on - strand, within Psyr_0530 at 599.917 kb on - strand, within Psyr_0531 at 599.941 kb on - strand, within Psyr_0531 at 600.013 kb on + strand, within Psyr_0531 at 600.014 kb on - strand, within Psyr_0531 at 600.824 kb on - strand, within Psyr_0531 at 600.834 kb on + strand, within Psyr_0531 at 601.166 kb on + strand at 601.167 kb on - strand at 601.192 kb on + strand at 601.192 kb on + strand at 601.193 kb on - strand at 601.193 kb on - strand at 601.237 kb on - strand at 601.441 kb on - strand, within Psyr_0532 at 601.449 kb on + strand, within Psyr_0532 at 601.449 kb on + strand, within Psyr_0532 at 601.449 kb on + strand, within Psyr_0532 at 601.450 kb on - strand, within Psyr_0532 at 601.450 kb on - strand, within Psyr_0532 at 601.450 kb on - strand, within Psyr_0532 at 601.450 kb on - strand, within Psyr_0532 at 601.465 kb on - strand, within Psyr_0532 at 601.465 kb on - strand, within Psyr_0532 at 601.465 kb on - strand, within Psyr_0532 at 601.588 kb on - strand, within Psyr_0532 at 601.669 kb on - strand, within Psyr_0532 at 601.750 kb on - strand, within Psyr_0532 at 601.750 kb on - strand, within Psyr_0532 at 601.809 kb on + strand, within Psyr_0532 at 601.809 kb on + strand, within Psyr_0532 at 601.809 kb on + strand, within Psyr_0532 at 601.809 kb on + strand, within Psyr_0532 at 601.810 kb on - strand, within Psyr_0532 at 601.810 kb on - strand, within Psyr_0532 at 601.810 kb on - strand, within Psyr_0532 at 602.000 kb on + strand, within Psyr_0532 at 602.030 kb on + strand, within Psyr_0532 at 602.144 kb on + strand at 602.151 kb on - strand at 602.151 kb on - strand at 602.151 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 598,799 - +1.0 599,218 - Psyr_0530 0.41 -0.4 599,917 - Psyr_0531 0.12 -1.4 599,941 - Psyr_0531 0.14 -2.2 600,013 + Psyr_0531 0.19 -0.4 600,014 - Psyr_0531 0.19 -0.6 600,824 - Psyr_0531 0.77 -0.4 600,834 + Psyr_0531 0.77 +1.5 601,166 + -0.2 601,167 - -1.0 601,192 + -1.4 601,192 + -1.0 601,193 - -0.7 601,193 - -1.0 601,237 - -1.0 601,441 - Psyr_0532 0.27 -0.6 601,449 + Psyr_0532 0.28 +0.1 601,449 + Psyr_0532 0.28 -0.3 601,449 + Psyr_0532 0.28 +0.1 601,450 - Psyr_0532 0.28 -0.6 601,450 - Psyr_0532 0.28 -0.4 601,450 - Psyr_0532 0.28 +0.5 601,450 - Psyr_0532 0.28 +1.0 601,465 - Psyr_0532 0.30 -2.6 601,465 - Psyr_0532 0.30 +0.4 601,465 - Psyr_0532 0.30 -2.0 601,588 - Psyr_0532 0.42 +0.4 601,669 - Psyr_0532 0.51 +0.2 601,750 - Psyr_0532 0.59 -0.6 601,750 - Psyr_0532 0.59 -0.0 601,809 + Psyr_0532 0.66 +0.3 601,809 + Psyr_0532 0.66 -1.6 601,809 + Psyr_0532 0.66 +0.4 601,809 + Psyr_0532 0.66 -0.0 601,810 - Psyr_0532 0.66 +0.4 601,810 - Psyr_0532 0.66 +2.0 601,810 - Psyr_0532 0.66 +1.4 602,000 + Psyr_0532 0.85 +2.0 602,030 + Psyr_0532 0.89 -1.1 602,144 + -0.7 602,151 - -2.0 602,151 - -1.9 602,151 - -0.2
Or see this region's nucleotide sequence