Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0528 and Psyr_0529 overlap by 17 nucleotides Psyr_0529 and Psyr_0530 overlap by 1 nucleotides Psyr_0530 and Psyr_0531 overlap by 4 nucleotides
Psyr_0528: Psyr_0528 - Carbamoyltransferase, at 595,978 to 597,735
_0528
Psyr_0529: Psyr_0529 - Glycosyl transferase, group 1, at 597,719 to 598,849
_0529
Psyr_0530: Psyr_0530 - Mig-14, at 598,849 to 599,745
_0530
Psyr_0531: Psyr_0531 - LmbE-like protein, at 599,742 to 601,151
_0531
Position (kb)
597
598
599 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 596.749 kb on + strand, within Psyr_0528 at 596.749 kb on + strand, within Psyr_0528 at 597.043 kb on + strand, within Psyr_0528 at 597.043 kb on + strand, within Psyr_0528 at 597.043 kb on + strand, within Psyr_0528 at 597.122 kb on + strand, within Psyr_0528 at 597.122 kb on + strand, within Psyr_0528 at 597.520 kb on + strand, within Psyr_0528 at 597.718 kb on + strand at 597.718 kb on + strand at 597.719 kb on - strand at 597.744 kb on + strand at 597.764 kb on + strand at 597.797 kb on + strand at 597.797 kb on + strand at 597.912 kb on - strand, within Psyr_0529 at 597.912 kb on - strand, within Psyr_0529 at 598.022 kb on + strand, within Psyr_0529 at 598.022 kb on + strand, within Psyr_0529 at 598.022 kb on + strand, within Psyr_0529 at 598.022 kb on + strand, within Psyr_0529 at 598.022 kb on + strand, within Psyr_0529 at 598.022 kb on + strand, within Psyr_0529 at 598.022 kb on + strand, within Psyr_0529 at 598.022 kb on + strand, within Psyr_0529 at 598.023 kb on - strand, within Psyr_0529 at 598.023 kb on - strand, within Psyr_0529 at 598.023 kb on - strand, within Psyr_0529 at 598.026 kb on + strand, within Psyr_0529 at 598.026 kb on + strand, within Psyr_0529 at 598.217 kb on + strand, within Psyr_0529 at 598.517 kb on + strand, within Psyr_0529 at 598.517 kb on + strand, within Psyr_0529 at 598.685 kb on - strand, within Psyr_0529 at 598.799 kb on - strand at 599.218 kb on - strand, within Psyr_0530
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 596,749 + Psyr_0528 0.44 -0.6 596,749 + Psyr_0528 0.44 -0.0 597,043 + Psyr_0528 0.61 -1.0 597,043 + Psyr_0528 0.61 +0.1 597,043 + Psyr_0528 0.61 -2.0 597,122 + Psyr_0528 0.65 -1.4 597,122 + Psyr_0528 0.65 -0.6 597,520 + Psyr_0528 0.88 +1.0 597,718 + +0.4 597,718 + -0.8 597,719 - +1.0 597,744 + -1.9 597,764 + -0.7 597,797 + -1.8 597,797 + -1.6 597,912 - Psyr_0529 0.17 -0.0 597,912 - Psyr_0529 0.17 -0.6 598,022 + Psyr_0529 0.27 -0.2 598,022 + Psyr_0529 0.27 +1.6 598,022 + Psyr_0529 0.27 +1.0 598,022 + Psyr_0529 0.27 -2.9 598,022 + Psyr_0529 0.27 -1.8 598,022 + Psyr_0529 0.27 -0.6 598,022 + Psyr_0529 0.27 -1.8 598,022 + Psyr_0529 0.27 -2.6 598,023 - Psyr_0529 0.27 -1.4 598,023 - Psyr_0529 0.27 +0.4 598,023 - Psyr_0529 0.27 -1.5 598,026 + Psyr_0529 0.27 -0.8 598,026 + Psyr_0529 0.27 -1.0 598,217 + Psyr_0529 0.44 -0.0 598,517 + Psyr_0529 0.71 +0.1 598,517 + Psyr_0529 0.71 -0.3 598,685 - Psyr_0529 0.85 -0.6 598,799 - +1.0 599,218 - Psyr_0530 0.41 -0.4
Or see this region's nucleotide sequence