Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0452 and Psyr_0453 are separated by 106 nucleotides Psyr_0453 and Psyr_0454 are separated by 131 nucleotides Psyr_0454 and Psyr_0455 are separated by 209 nucleotides
Psyr_0452: Psyr_0452 - HylII, at 487,976 to 488,590
_0452
Psyr_0453: Psyr_0453 - 16S rRNA m(3)U-1498 methyltransferase, at 488,697 to 489,416
_0453
Psyr_0454: Psyr_0454 - adenosylmethionine-8-amino-7-oxononanoate aminotransferase apoenzyme, at 489,548 to 490,954
_0454
Psyr_0455: Psyr_0455 - Amine oxidase, at 491,164 to 493,038
_0455
Position (kb) 489
490
491 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 488.579 kb on + strand at 488.579 kb on + strand at 488.579 kb on + strand at 488.580 kb on - strand at 488.580 kb on - strand at 489.098 kb on + strand, within Psyr_0453 at 489.099 kb on - strand, within Psyr_0453 at 489.099 kb on - strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.341 kb on - strand, within Psyr_0453 at 489.341 kb on - strand, within Psyr_0453 at 489.341 kb on - strand, within Psyr_0453 at 489.341 kb on - strand, within Psyr_0453 at 489.341 kb on - strand, within Psyr_0453 at 489.341 kb on - strand, within Psyr_0453 at 489.549 kb on + strand at 489.678 kb on + strand at 489.679 kb on - strand at 489.679 kb on - strand at 489.694 kb on - strand, within Psyr_0454 at 489.694 kb on - strand, within Psyr_0454 at 489.694 kb on - strand, within Psyr_0454 at 489.694 kb on - strand, within Psyr_0454 at 489.694 kb on - strand, within Psyr_0454 at 489.991 kb on - strand, within Psyr_0454 at 489.991 kb on - strand, within Psyr_0454 at 489.991 kb on - strand, within Psyr_0454 at 489.991 kb on - strand, within Psyr_0454 at 490.035 kb on + strand, within Psyr_0454 at 490.035 kb on + strand, within Psyr_0454 at 490.048 kb on - strand, within Psyr_0454 at 490.087 kb on - strand, within Psyr_0454 at 490.087 kb on - strand, within Psyr_0454 at 490.245 kb on + strand, within Psyr_0454 at 490.245 kb on + strand, within Psyr_0454 at 490.257 kb on + strand, within Psyr_0454 at 490.257 kb on + strand, within Psyr_0454 at 490.257 kb on + strand, within Psyr_0454 at 490.258 kb on - strand, within Psyr_0454 at 490.258 kb on - strand, within Psyr_0454 at 490.258 kb on - strand, within Psyr_0454 at 490.258 kb on - strand, within Psyr_0454 at 490.258 kb on - strand, within Psyr_0454 at 490.258 kb on - strand, within Psyr_0454 at 490.402 kb on - strand, within Psyr_0454 at 490.450 kb on - strand, within Psyr_0454 at 491.010 kb on + strand at 491.051 kb on - strand at 491.077 kb on + strand at 491.133 kb on + strand at 491.134 kb on - strand at 491.134 kb on - strand at 491.142 kb on + strand at 491.143 kb on - strand at 491.199 kb on - strand at 491.293 kb on + strand at 491.294 kb on - strand at 491.479 kb on + strand, within Psyr_0455 at 491.480 kb on - strand, within Psyr_0455 at 491.480 kb on - strand, within Psyr_0455 at 491.497 kb on + strand, within Psyr_0455 at 491.497 kb on + strand, within Psyr_0455 at 491.497 kb on + strand, within Psyr_0455 at 491.497 kb on + strand, within Psyr_0455 at 491.497 kb on + strand, within Psyr_0455 at 491.497 kb on + strand, within Psyr_0455 at 491.497 kb on + strand, within Psyr_0455 at 491.498 kb on - strand, within Psyr_0455 at 491.498 kb on - strand, within Psyr_0455 at 491.498 kb on - strand, within Psyr_0455 at 491.498 kb on - strand, within Psyr_0455 at 491.498 kb on - strand, within Psyr_0455 at 491.498 kb on - strand, within Psyr_0455 at 491.498 kb on - strand, within Psyr_0455 at 491.498 kb on - strand, within Psyr_0455 at 491.557 kb on + strand, within Psyr_0455 at 491.558 kb on - strand, within Psyr_0455 at 491.558 kb on - strand, within Psyr_0455 at 491.756 kb on - strand, within Psyr_0455 at 491.780 kb on - strand, within Psyr_0455 at 491.947 kb on + strand, within Psyr_0455 at 491.947 kb on + strand, within Psyr_0455 at 491.947 kb on + strand, within Psyr_0455 at 491.947 kb on + strand, within Psyr_0455 at 491.947 kb on + strand, within Psyr_0455 at 491.948 kb on - strand, within Psyr_0455
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 488,579 + -0.4 488,579 + +0.6 488,579 + -0.6 488,580 - -0.1 488,580 - +0.7 489,098 + Psyr_0453 0.56 +0.9 489,099 - Psyr_0453 0.56 +1.0 489,099 - Psyr_0453 0.56 -0.3 489,340 + Psyr_0453 0.89 -0.3 489,340 + Psyr_0453 0.89 +0.1 489,340 + Psyr_0453 0.89 +0.7 489,340 + Psyr_0453 0.89 -0.3 489,340 + Psyr_0453 0.89 +0.1 489,340 + Psyr_0453 0.89 +1.0 489,340 + Psyr_0453 0.89 +1.0 489,340 + Psyr_0453 0.89 -0.7 489,341 - Psyr_0453 0.89 +0.4 489,341 - Psyr_0453 0.89 -0.6 489,341 - Psyr_0453 0.89 -1.3 489,341 - Psyr_0453 0.89 +1.0 489,341 - Psyr_0453 0.89 -0.6 489,341 - Psyr_0453 0.89 +1.1 489,549 + +0.2 489,678 + -1.6 489,679 - -2.6 489,679 - -2.0 489,694 - Psyr_0454 0.10 -1.0 489,694 - Psyr_0454 0.10 +0.0 489,694 - Psyr_0454 0.10 +0.0 489,694 - Psyr_0454 0.10 +1.0 489,694 - Psyr_0454 0.10 -1.3 489,991 - Psyr_0454 0.31 -0.6 489,991 - Psyr_0454 0.31 -0.6 489,991 - Psyr_0454 0.31 -2.4 489,991 - Psyr_0454 0.31 -1.6 490,035 + Psyr_0454 0.35 -0.6 490,035 + Psyr_0454 0.35 -0.6 490,048 - Psyr_0454 0.36 -1.3 490,087 - Psyr_0454 0.38 -1.3 490,087 - Psyr_0454 0.38 -1.0 490,245 + Psyr_0454 0.50 -1.3 490,245 + Psyr_0454 0.50 -1.0 490,257 + Psyr_0454 0.50 -1.8 490,257 + Psyr_0454 0.50 -2.9 490,257 + Psyr_0454 0.50 -1.0 490,258 - Psyr_0454 0.50 +0.0 490,258 - Psyr_0454 0.50 +2.0 490,258 - Psyr_0454 0.50 -2.8 490,258 - Psyr_0454 0.50 -2.3 490,258 - Psyr_0454 0.50 -1.8 490,258 - Psyr_0454 0.50 -1.0 490,402 - Psyr_0454 0.61 -3.2 490,450 - Psyr_0454 0.64 +0.0 491,010 + -0.1 491,051 - +0.4 491,077 + +0.0 491,133 + -0.2 491,134 - +0.8 491,134 - +2.8 491,142 + +1.7 491,143 - +0.7 491,199 - +0.0 491,293 + +0.4 491,294 - -0.7 491,479 + Psyr_0455 0.17 +1.0 491,480 - Psyr_0455 0.17 -0.0 491,480 - Psyr_0455 0.17 +0.8 491,497 + Psyr_0455 0.18 +1.7 491,497 + Psyr_0455 0.18 +0.2 491,497 + Psyr_0455 0.18 +0.9 491,497 + Psyr_0455 0.18 +0.9 491,497 + Psyr_0455 0.18 +0.3 491,497 + Psyr_0455 0.18 -0.2 491,497 + Psyr_0455 0.18 +0.6 491,498 - Psyr_0455 0.18 -0.1 491,498 - Psyr_0455 0.18 -0.3 491,498 - Psyr_0455 0.18 +0.5 491,498 - Psyr_0455 0.18 -0.3 491,498 - Psyr_0455 0.18 -0.8 491,498 - Psyr_0455 0.18 +0.2 491,498 - Psyr_0455 0.18 -0.3 491,498 - Psyr_0455 0.18 -0.1 491,557 + Psyr_0455 0.21 -0.2 491,558 - Psyr_0455 0.21 +0.0 491,558 - Psyr_0455 0.21 -1.0 491,756 - Psyr_0455 0.32 +0.3 491,780 - Psyr_0455 0.33 +0.3 491,947 + Psyr_0455 0.42 +1.4 491,947 + Psyr_0455 0.42 +0.0 491,947 + Psyr_0455 0.42 -1.2 491,947 + Psyr_0455 0.42 +1.8 491,947 + Psyr_0455 0.42 +1.1 491,948 - Psyr_0455 0.42 +0.8
Or see this region's nucleotide sequence