Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0394 and Psyr_0395 are separated by 66 nucleotides Psyr_0395 and Psyr_0396 are separated by 367 nucleotides
Psyr_0394: Psyr_0394 - Heat shock protein HslU, at 420,627 to 421,964
_0394
Psyr_0395: Psyr_0395 - HslV component of HslUV peptidase, Threonine peptidase, MEROPS family T01B, at 422,031 to 422,609
_0395
Psyr_0396: Psyr_0396 - Sporulation related protein, at 422,977 to 423,672
_0396
Position (kb) 422
423 Strain fitness (log2 ratio) -2
-1
0
1
2 at 421.175 kb on + strand, within Psyr_0394 at 421.175 kb on + strand, within Psyr_0394 at 421.175 kb on + strand, within Psyr_0394 at 421.176 kb on - strand, within Psyr_0394 at 421.680 kb on + strand, within Psyr_0394 at 421.681 kb on - strand, within Psyr_0394 at 421.696 kb on + strand, within Psyr_0394 at 421.697 kb on - strand, within Psyr_0394 at 421.825 kb on - strand, within Psyr_0394 at 421.825 kb on - strand, within Psyr_0394 at 421.919 kb on + strand at 421.920 kb on - strand at 421.985 kb on + strand at 422.446 kb on + strand, within Psyr_0395 at 422.447 kb on - strand, within Psyr_0395 at 422.458 kb on - strand, within Psyr_0395 at 422.458 kb on - strand, within Psyr_0395 at 422.458 kb on - strand, within Psyr_0395 at 422.528 kb on + strand, within Psyr_0395 at 422.628 kb on - strand at 422.665 kb on + strand at 422.666 kb on - strand at 422.666 kb on - strand at 422.687 kb on + strand at 422.842 kb on - strand at 422.842 kb on - strand at 422.842 kb on - strand at 422.901 kb on - strand at 422.901 kb on - strand at 422.932 kb on - strand at 423.013 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 421,175 + Psyr_0394 0.41 +1.0 421,175 + Psyr_0394 0.41 -1.0 421,175 + Psyr_0394 0.41 -1.1 421,176 - Psyr_0394 0.41 -0.6 421,680 + Psyr_0394 0.79 +2.2 421,681 - Psyr_0394 0.79 -2.4 421,696 + Psyr_0394 0.80 -0.7 421,697 - Psyr_0394 0.80 -1.3 421,825 - Psyr_0394 0.90 -0.2 421,825 - Psyr_0394 0.90 +1.0 421,919 + +0.0 421,920 - -0.1 421,985 + -0.3 422,446 + Psyr_0395 0.72 -0.0 422,447 - Psyr_0395 0.72 -1.6 422,458 - Psyr_0395 0.74 -0.7 422,458 - Psyr_0395 0.74 +0.0 422,458 - Psyr_0395 0.74 -0.6 422,528 + Psyr_0395 0.86 -1.6 422,628 - +0.0 422,665 + +1.6 422,666 - -1.4 422,666 - -0.7 422,687 + +1.0 422,842 - +0.7 422,842 - +0.5 422,842 - +0.8 422,901 - +0.4 422,901 - +0.0 422,932 - -0.4 423,013 - -0.6
Or see this region's nucleotide sequence