Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0302 and Psyr_0303 overlap by 1 nucleotides Psyr_0303 and Psyr_0304 are separated by 208 nucleotides Psyr_0304 and Psyr_0305 are separated by 0 nucleotides
Psyr_0302: Psyr_0302 - 3-octaprenyl-4hydroxybenzoate decarboxylase, at 321,695 to 323,173
_0302
Psyr_0303: Psyr_0303 - Ferredoxin:Oxidoreductase FAD/NAD(P)-binding protein, at 323,173 to 324,141
_0303
Psyr_0304: Psyr_0304 - ChaC-like protein, at 324,350 to 325,018
_0304
Psyr_0305: Psyr_0305 - GlpT transporter:Glycerol-3-phosphate transporter, at 325,019 to 326,368
_0305
Position (kb) 323
324
325 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 323.166 kb on + strand at 323.180 kb on + strand at 323.258 kb on - strand at 323.280 kb on + strand, within Psyr_0303 at 323.367 kb on + strand, within Psyr_0303 at 323.551 kb on + strand, within Psyr_0303 at 323.849 kb on - strand, within Psyr_0303 at 323.879 kb on - strand, within Psyr_0303 at 324.055 kb on + strand at 324.055 kb on + strand at 324.056 kb on - strand at 324.056 kb on - strand at 324.116 kb on + strand at 324.116 kb on + strand at 324.117 kb on - strand at 324.172 kb on + strand at 324.173 kb on - strand at 324.190 kb on + strand at 324.190 kb on + strand at 324.191 kb on - strand at 324.191 kb on - strand at 324.230 kb on + strand at 324.231 kb on - strand at 324.231 kb on - strand at 324.231 kb on - strand at 324.231 kb on - strand at 324.231 kb on - strand at 324.515 kb on + strand, within Psyr_0304 at 324.596 kb on + strand, within Psyr_0304 at 324.597 kb on - strand, within Psyr_0304 at 324.645 kb on + strand, within Psyr_0304 at 324.804 kb on + strand, within Psyr_0304 at 324.805 kb on - strand, within Psyr_0304 at 324.845 kb on + strand, within Psyr_0304 at 324.846 kb on - strand, within Psyr_0304 at 324.846 kb on - strand, within Psyr_0304 at 324.846 kb on - strand, within Psyr_0304 at 324.846 kb on - strand, within Psyr_0304
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 323,166 + +0.0 323,180 + -1.4 323,258 - -2.0 323,280 + Psyr_0303 0.11 -0.5 323,367 + Psyr_0303 0.20 -2.1 323,551 + Psyr_0303 0.39 -1.6 323,849 - Psyr_0303 0.70 -1.6 323,879 - Psyr_0303 0.73 -1.9 324,055 + -0.6 324,055 + -0.3 324,056 - +1.0 324,056 - -2.1 324,116 + -0.3 324,116 + -3.0 324,117 - -0.7 324,172 + -0.6 324,173 - -0.3 324,190 + +1.4 324,190 + -0.5 324,191 - -0.3 324,191 - +0.3 324,230 + -1.8 324,231 - +0.4 324,231 - +0.6 324,231 - -1.0 324,231 - +0.2 324,231 - -1.6 324,515 + Psyr_0304 0.25 -1.1 324,596 + Psyr_0304 0.37 +1.3 324,597 - Psyr_0304 0.37 -0.1 324,645 + Psyr_0304 0.44 -0.2 324,804 + Psyr_0304 0.68 -0.4 324,805 - Psyr_0304 0.68 +1.8 324,845 + Psyr_0304 0.74 -2.3 324,846 - Psyr_0304 0.74 -1.7 324,846 - Psyr_0304 0.74 -0.5 324,846 - Psyr_0304 0.74 -0.8 324,846 - Psyr_0304 0.74 +0.2
Or see this region's nucleotide sequence