Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0254 and Psyr_0255 are separated by 314 nucleotides Psyr_0255 and Psyr_0256 are separated by 226 nucleotides Psyr_0256 and Psyr_0257 overlap by 1 nucleotides
Psyr_0254: Psyr_0254 - N-acetylglutamate synthase, at 267,685 to 268,983
_0254
Psyr_0255: Psyr_0255 - glutamate-cysteine ligase, at 269,298 to 270,905
_0255
Psyr_0256: Psyr_0256 - Phenylacetic acid degradation-related protein, at 271,132 to 271,515
_0256
Psyr_0257: Psyr_0257 - RNA binding S1, at 271,515 to 273,833
_0257
Position (kb) 269
270
271 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 268.979 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.998 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.001 kb on - strand at 269.001 kb on - strand at 269.001 kb on - strand at 269.130 kb on - strand at 269.130 kb on - strand at 269.243 kb on + strand at 269.287 kb on + strand at 269.287 kb on + strand at 269.287 kb on + strand at 269.287 kb on + strand at 269.287 kb on + strand at 269.287 kb on + strand at 269.288 kb on - strand at 269.288 kb on - strand at 269.288 kb on - strand at 269.288 kb on - strand at 269.288 kb on - strand at 269.288 kb on - strand at 269.288 kb on - strand at 269.329 kb on + strand at 270.128 kb on - strand, within Psyr_0255 at 270.205 kb on + strand, within Psyr_0255 at 270.205 kb on + strand, within Psyr_0255 at 270.296 kb on - strand, within Psyr_0255 at 270.296 kb on - strand, within Psyr_0255 at 270.500 kb on - strand, within Psyr_0255 at 270.896 kb on - strand at 270.896 kb on - strand at 270.944 kb on + strand at 270.944 kb on + strand at 270.971 kb on - strand at 271.022 kb on - strand at 271.065 kb on - strand at 271.088 kb on + strand at 271.133 kb on + strand at 271.133 kb on + strand at 271.134 kb on - strand at 271.134 kb on - strand at 271.134 kb on - strand at 271.274 kb on + strand, within Psyr_0256 at 271.274 kb on + strand, within Psyr_0256 at 271.274 kb on + strand, within Psyr_0256 at 271.274 kb on + strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 268,979 + -0.2 268,981 + -2.8 268,981 + -0.6 268,981 + -1.3 268,981 + +1.0 268,981 + +1.7 268,981 + -0.2 268,981 + -1.0 268,981 + -1.0 268,981 + -0.1 268,981 + +1.8 268,981 + +1.5 268,981 + +0.9 268,981 + +0.3 268,981 + -1.8 268,982 - +0.3 268,982 - +0.2 268,982 - -0.0 268,982 - -0.3 268,982 - -0.1 268,982 - -0.3 268,982 - -0.3 268,982 - +2.0 268,982 - +0.0 268,998 + -0.8 269,000 + -1.0 269,000 + -0.3 269,000 + -0.1 269,000 + +1.3 269,000 + -1.0 269,000 + -0.1 269,000 + +0.3 269,000 + +2.8 269,000 + -0.9 269,001 - -0.1 269,001 - -0.4 269,001 - -2.3 269,130 - -0.9 269,130 - -0.5 269,243 + -1.5 269,287 + +0.0 269,287 + +0.0 269,287 + -0.5 269,287 + -0.1 269,287 + +0.2 269,287 + -0.4 269,288 - +0.4 269,288 - +0.5 269,288 - -0.3 269,288 - +0.1 269,288 - +1.6 269,288 - +0.7 269,288 - +0.0 269,329 + +0.2 270,128 - Psyr_0255 0.52 -0.8 270,205 + Psyr_0255 0.56 -1.3 270,205 + Psyr_0255 0.56 +1.0 270,296 - Psyr_0255 0.62 +1.0 270,296 - Psyr_0255 0.62 -1.5 270,500 - Psyr_0255 0.75 -0.5 270,896 - -1.3 270,896 - -0.3 270,944 + +0.0 270,944 + -0.3 270,971 - -0.0 271,022 - -0.8 271,065 - +0.4 271,088 + +0.0 271,133 + +0.5 271,133 + -1.1 271,134 - +0.2 271,134 - -0.4 271,134 - +0.2 271,274 + Psyr_0256 0.37 +0.2 271,274 + Psyr_0256 0.37 +0.1 271,274 + Psyr_0256 0.37 +0.2 271,274 + Psyr_0256 0.37 -0.2 271,275 - Psyr_0256 0.37 -0.3 271,275 - Psyr_0256 0.37 -1.4 271,275 - Psyr_0256 0.37 -1.5 271,275 - Psyr_0256 0.37 -0.2 271,275 - Psyr_0256 0.37 +0.4 271,275 - Psyr_0256 0.37 +1.5 271,275 - Psyr_0256 0.37 +0.0 271,275 - Psyr_0256 0.37 +0.2 271,275 - Psyr_0256 0.37 +0.0 271,275 - Psyr_0256 0.37 +0.0
Or see this region's nucleotide sequence