Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0215

Experiment: KB with Trimethoprim 125 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0213 and Psyr_0214 are separated by 21 nucleotidesPsyr_0214 and Psyr_0215 are separated by 69 nucleotidesPsyr_0215 and Psyr_0216 are separated by 82 nucleotidesPsyr_0216 and Psyr_0217 are separated by 10 nucleotides Psyr_0213: Psyr_0213 - RNAse PH, at 227,415 to 228,137 _0213 Psyr_0214: Psyr_0214 - conserved hypothetical protein, at 228,159 to 228,527 _0214 Psyr_0215: Psyr_0215 - Exodeoxyribonuclease III xth, at 228,597 to 229,376 _0215 Psyr_0216: Psyr_0216 - orotate phosphoribosyltransferase, at 229,459 to 230,100 _0216 Psyr_0217: Psyr_0217 - conserved hypothetical protein, at 230,111 to 230,743 _0217 Position (kb) 228 229 230Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 227.624 kb on + strand, within Psyr_0213at 227.637 kb on - strand, within Psyr_0213at 227.675 kb on + strand, within Psyr_0213at 227.676 kb on - strand, within Psyr_0213at 227.676 kb on - strand, within Psyr_0213at 227.676 kb on - strand, within Psyr_0213at 227.676 kb on - strand, within Psyr_0213at 227.754 kb on + strand, within Psyr_0213at 227.754 kb on + strand, within Psyr_0213at 227.754 kb on + strand, within Psyr_0213at 227.754 kb on + strand, within Psyr_0213at 227.754 kb on + strand, within Psyr_0213at 227.755 kb on - strand, within Psyr_0213at 227.755 kb on - strand, within Psyr_0213at 227.755 kb on - strand, within Psyr_0213at 227.804 kb on + strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.807 kb on + strand, within Psyr_0213at 227.919 kb on + strand, within Psyr_0213at 227.919 kb on + strand, within Psyr_0213at 227.919 kb on + strand, within Psyr_0213at 227.919 kb on + strand, within Psyr_0213at 227.920 kb on - strand, within Psyr_0213at 227.920 kb on - strand, within Psyr_0213at 227.920 kb on - strand, within Psyr_0213at 227.920 kb on - strand, within Psyr_0213at 227.920 kb on - strand, within Psyr_0213at 227.949 kb on + strand, within Psyr_0213at 227.950 kb on - strand, within Psyr_0213at 227.950 kb on - strand, within Psyr_0213at 228.284 kb on + strand, within Psyr_0214at 228.284 kb on + strand, within Psyr_0214at 228.470 kb on - strand, within Psyr_0214at 228.492 kb on + strandat 228.492 kb on + strandat 228.492 kb on + strandat 228.493 kb on - strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.603 kb on - strandat 228.603 kb on - strandat 228.603 kb on - strandat 228.619 kb on + strandat 228.715 kb on + strand, within Psyr_0215at 228.716 kb on - strand, within Psyr_0215at 228.776 kb on - strand, within Psyr_0215at 228.776 kb on - strand, within Psyr_0215at 228.928 kb on + strand, within Psyr_0215at 228.928 kb on + strand, within Psyr_0215at 228.928 kb on + strand, within Psyr_0215at 228.928 kb on + strand, within Psyr_0215at 228.928 kb on + strand, within Psyr_0215at 228.928 kb on + strand, within Psyr_0215at 228.929 kb on - strand, within Psyr_0215at 228.929 kb on - strand, within Psyr_0215at 228.929 kb on - strand, within Psyr_0215at 228.929 kb on - strand, within Psyr_0215at 228.929 kb on - strand, within Psyr_0215at 228.929 kb on - strand, within Psyr_0215at 228.929 kb on - strand, within Psyr_0215at 228.943 kb on + strand, within Psyr_0215at 228.943 kb on + strand, within Psyr_0215at 228.943 kb on + strand, within Psyr_0215at 228.944 kb on - strand, within Psyr_0215at 228.944 kb on - strand, within Psyr_0215at 228.944 kb on - strand, within Psyr_0215at 228.944 kb on - strand, within Psyr_0215at 228.944 kb on - strand, within Psyr_0215at 228.949 kb on + strand, within Psyr_0215at 228.950 kb on - strand, within Psyr_0215at 228.950 kb on - strand, within Psyr_0215at 228.950 kb on - strand, within Psyr_0215at 228.950 kb on - strand, within Psyr_0215at 228.950 kb on - strand, within Psyr_0215at 228.950 kb on - strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.983 kb on - strand, within Psyr_0215at 228.983 kb on - strand, within Psyr_0215at 228.983 kb on - strand, within Psyr_0215at 228.983 kb on - strand, within Psyr_0215at 228.983 kb on - strand, within Psyr_0215at 229.166 kb on - strand, within Psyr_0215at 229.166 kb on - strand, within Psyr_0215at 229.210 kb on + strand, within Psyr_0215at 229.210 kb on + strand, within Psyr_0215at 229.211 kb on - strand, within Psyr_0215at 229.219 kb on + strand, within Psyr_0215at 229.219 kb on + strand, within Psyr_0215at 229.219 kb on + strand, within Psyr_0215at 229.220 kb on - strand, within Psyr_0215at 229.220 kb on - strand, within Psyr_0215at 229.278 kb on + strand, within Psyr_0215at 229.278 kb on + strand, within Psyr_0215at 229.353 kb on + strandat 229.353 kb on + strandat 229.354 kb on - strandat 229.354 kb on - strandat 229.354 kb on - strandat 229.354 kb on - strandat 229.376 kb on + strandat 229.403 kb on + strandat 229.403 kb on + strandat 229.403 kb on + strandat 229.403 kb on + strandat 229.404 kb on - strandat 229.404 kb on - strandat 229.420 kb on - strandat 229.423 kb on + strandat 229.423 kb on + strandat 229.423 kb on + strandat 229.423 kb on + strandat 229.423 kb on + strandat 229.427 kb on + strandat 229.427 kb on + strandat 229.428 kb on - strandat 229.442 kb on + strandat 229.672 kb on + strand, within Psyr_0216at 230.238 kb on - strand, within Psyr_0217at 230.297 kb on + strand, within Psyr_0217at 230.297 kb on + strand, within Psyr_0217at 230.298 kb on - strand, within Psyr_0217at 230.298 kb on - strand, within Psyr_0217at 230.298 kb on - strand, within Psyr_0217at 230.298 kb on - strand, within Psyr_0217at 230.313 kb on - strand, within Psyr_0217at 230.365 kb on + strand, within Psyr_0217

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml
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227,624 + Psyr_0213 0.29 +0.2
227,637 - Psyr_0213 0.31 -0.2
227,675 + Psyr_0213 0.36 +0.7
227,676 - Psyr_0213 0.36 +0.1
227,676 - Psyr_0213 0.36 -0.3
227,676 - Psyr_0213 0.36 +0.5
227,676 - Psyr_0213 0.36 -0.0
227,754 + Psyr_0213 0.47 -0.3
227,754 + Psyr_0213 0.47 +1.8
227,754 + Psyr_0213 0.47 +0.1
227,754 + Psyr_0213 0.47 +0.1
227,754 + Psyr_0213 0.47 -0.3
227,755 - Psyr_0213 0.47 -1.4
227,755 - Psyr_0213 0.47 -0.2
227,755 - Psyr_0213 0.47 -0.5
227,804 + Psyr_0213 0.54 -1.6
227,805 - Psyr_0213 0.54 +0.6
227,805 - Psyr_0213 0.54 -0.2
227,805 - Psyr_0213 0.54 -0.0
227,805 - Psyr_0213 0.54 +0.5
227,805 - Psyr_0213 0.54 +0.3
227,805 - Psyr_0213 0.54 -0.1
227,805 - Psyr_0213 0.54 +0.6
227,807 + Psyr_0213 0.54 -0.7
227,919 + Psyr_0213 0.70 -0.5
227,919 + Psyr_0213 0.70 +0.2
227,919 + Psyr_0213 0.70 +0.1
227,919 + Psyr_0213 0.70 +0.7
227,920 - Psyr_0213 0.70 +0.9
227,920 - Psyr_0213 0.70 +0.1
227,920 - Psyr_0213 0.70 -1.3
227,920 - Psyr_0213 0.70 +0.2
227,920 - Psyr_0213 0.70 -2.8
227,949 + Psyr_0213 0.74 +0.2
227,950 - Psyr_0213 0.74 -0.6
227,950 - Psyr_0213 0.74 -0.2
228,284 + Psyr_0214 0.34 +0.2
228,284 + Psyr_0214 0.34 +0.5
228,470 - Psyr_0214 0.84 +1.6
228,492 + -1.1
228,492 + -0.2
228,492 + -0.2
228,493 - -2.4
228,525 + +0.9
228,525 + +0.7
228,525 + -0.3
228,525 + -0.1
228,525 + +1.6
228,525 + -1.8
228,525 + -1.9
228,525 + +0.2
228,525 + -0.3
228,526 - +0.8
228,526 - +0.4
228,526 - -1.5
228,526 - -0.5
228,526 - -0.8
228,526 - -0.3
228,526 - -0.4
228,526 - -0.4
228,526 - -0.9
228,603 - +0.1
228,603 - +3.1
228,603 - -1.0
228,619 + -2.2
228,715 + Psyr_0215 0.15 -2.9
228,716 - Psyr_0215 0.15 -1.0
228,776 - Psyr_0215 0.23 -0.7
228,776 - Psyr_0215 0.23 -0.1
228,928 + Psyr_0215 0.42 +0.1
228,928 + Psyr_0215 0.42 -0.2
228,928 + Psyr_0215 0.42 -1.8
228,928 + Psyr_0215 0.42 -0.7
228,928 + Psyr_0215 0.42 -1.3
228,928 + Psyr_0215 0.42 -0.9
228,929 - Psyr_0215 0.43 -0.5
228,929 - Psyr_0215 0.43 -0.4
228,929 - Psyr_0215 0.43 +0.4
228,929 - Psyr_0215 0.43 -1.0
228,929 - Psyr_0215 0.43 -1.3
228,929 - Psyr_0215 0.43 +2.3
228,929 - Psyr_0215 0.43 +1.3
228,943 + Psyr_0215 0.44 -1.0
228,943 + Psyr_0215 0.44 -2.1
228,943 + Psyr_0215 0.44 -1.4
228,944 - Psyr_0215 0.44 -1.2
228,944 - Psyr_0215 0.44 +1.1
228,944 - Psyr_0215 0.44 -0.2
228,944 - Psyr_0215 0.44 -0.8
228,944 - Psyr_0215 0.44 -0.5
228,949 + Psyr_0215 0.45 -3.5
228,950 - Psyr_0215 0.45 -0.0
228,950 - Psyr_0215 0.45 -0.7
228,950 - Psyr_0215 0.45 -0.9
228,950 - Psyr_0215 0.45 -0.6
228,950 - Psyr_0215 0.45 -1.3
228,950 - Psyr_0215 0.45 +0.3
228,982 + Psyr_0215 0.49 -0.6
228,982 + Psyr_0215 0.49 -1.4
228,982 + Psyr_0215 0.49 -0.6
228,982 + Psyr_0215 0.49 -2.4
228,982 + Psyr_0215 0.49 -0.8
228,982 + Psyr_0215 0.49 -1.1
228,982 + Psyr_0215 0.49 -1.3
228,982 + Psyr_0215 0.49 -1.3
228,982 + Psyr_0215 0.49 +0.5
228,982 + Psyr_0215 0.49 -0.3
228,982 + Psyr_0215 0.49 -3.0
228,983 - Psyr_0215 0.49 -0.6
228,983 - Psyr_0215 0.49 +0.1
228,983 - Psyr_0215 0.49 +0.7
228,983 - Psyr_0215 0.49 -0.5
228,983 - Psyr_0215 0.49 -0.9
229,166 - Psyr_0215 0.73 -0.5
229,166 - Psyr_0215 0.73 -2.3
229,210 + Psyr_0215 0.79 -1.3
229,210 + Psyr_0215 0.79 -0.6
229,211 - Psyr_0215 0.79 -0.4
229,219 + Psyr_0215 0.80 -0.3
229,219 + Psyr_0215 0.80 -1.0
229,219 + Psyr_0215 0.80 -0.7
229,220 - Psyr_0215 0.80 -0.2
229,220 - Psyr_0215 0.80 -1.1
229,278 + Psyr_0215 0.87 -0.1
229,278 + Psyr_0215 0.87 -0.4
229,353 + -1.8
229,353 + +0.8
229,354 - -0.8
229,354 - -0.9
229,354 - -0.1
229,354 - -0.3
229,376 + -2.1
229,403 + -0.5
229,403 + -0.9
229,403 + -0.7
229,403 + -0.9
229,404 - -0.9
229,404 - +1.1
229,420 - -2.5
229,423 + -0.3
229,423 + -0.3
229,423 + -1.5
229,423 + +0.1
229,423 + +0.9
229,427 + -1.3
229,427 + -0.0
229,428 - -0.5
229,442 + +0.1
229,672 + Psyr_0216 0.33 -0.5
230,238 - Psyr_0217 0.20 +0.9
230,297 + Psyr_0217 0.29 +0.7
230,297 + Psyr_0217 0.29 -0.1
230,298 - Psyr_0217 0.30 +0.9
230,298 - Psyr_0217 0.30 -0.6
230,298 - Psyr_0217 0.30 -0.3
230,298 - Psyr_0217 0.30 +0.8
230,313 - Psyr_0217 0.32 -0.3
230,365 + Psyr_0217 0.40 +0.3

Or see this region's nucleotide sequence