Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0202

Experiment: KB with Trimethoprim 125 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0201 and Psyr_0202 are separated by 11 nucleotidesPsyr_0202 and Psyr_0203 are separated by 92 nucleotidesPsyr_0203 and Psyr_0204 overlap by 4 nucleotides Psyr_0201: Psyr_0201 - ATP-dependent DNA helicase RecG, at 215,460 to 217,535 _0201 Psyr_0202: Psyr_0202 - transcriptional regulator, LysR family, at 217,547 to 218,470 _0202 Psyr_0203: Psyr_0203 - outer membrane transport energization protein TonB, at 218,563 to 219,309 _0203 Psyr_0204: Psyr_0204 - outer membrane transport energization protein ExbD, at 219,306 to 219,731 _0204 Position (kb) 217 218 219Strain fitness (log2 ratio) -3 -2 -1 0 1at 216.643 kb on - strand, within Psyr_0201at 216.692 kb on - strand, within Psyr_0201at 217.087 kb on + strand, within Psyr_0201at 217.087 kb on + strand, within Psyr_0201at 217.156 kb on + strand, within Psyr_0201at 217.156 kb on + strand, within Psyr_0201at 217.156 kb on + strand, within Psyr_0201at 217.157 kb on - strand, within Psyr_0201at 217.252 kb on + strand, within Psyr_0201at 217.253 kb on - strand, within Psyr_0201at 217.548 kb on + strandat 217.548 kb on + strandat 217.549 kb on - strandat 217.549 kb on - strandat 217.553 kb on - strandat 217.553 kb on - strandat 217.802 kb on + strand, within Psyr_0202at 217.802 kb on + strand, within Psyr_0202at 217.802 kb on + strand, within Psyr_0202at 217.802 kb on + strand, within Psyr_0202at 217.802 kb on + strand, within Psyr_0202at 217.803 kb on - strand, within Psyr_0202at 217.803 kb on - strand, within Psyr_0202at 217.803 kb on - strand, within Psyr_0202at 217.803 kb on - strand, within Psyr_0202at 217.803 kb on - strand, within Psyr_0202at 217.803 kb on - strand, within Psyr_0202at 217.889 kb on + strand, within Psyr_0202at 217.890 kb on - strand, within Psyr_0202at 218.103 kb on + strand, within Psyr_0202at 218.103 kb on + strand, within Psyr_0202at 218.103 kb on + strand, within Psyr_0202at 218.104 kb on - strand, within Psyr_0202at 218.104 kb on - strand, within Psyr_0202at 218.104 kb on - strand, within Psyr_0202at 218.104 kb on - strand, within Psyr_0202at 218.160 kb on + strand, within Psyr_0202at 218.160 kb on + strand, within Psyr_0202at 218.161 kb on - strand, within Psyr_0202at 218.161 kb on - strand, within Psyr_0202at 218.175 kb on + strand, within Psyr_0202at 218.175 kb on + strand, within Psyr_0202at 218.175 kb on + strand, within Psyr_0202at 218.175 kb on + strand, within Psyr_0202at 218.175 kb on + strand, within Psyr_0202at 218.176 kb on - strand, within Psyr_0202at 218.176 kb on - strand, within Psyr_0202at 218.176 kb on - strand, within Psyr_0202at 218.176 kb on - strand, within Psyr_0202at 218.176 kb on - strand, within Psyr_0202at 218.449 kb on - strandat 218.449 kb on - strandat 218.449 kb on - strandat 218.449 kb on - strandat 218.449 kb on - strandat 218.449 kb on - strandat 218.462 kb on - strandat 218.470 kb on + strandat 218.471 kb on - strandat 218.502 kb on + strandat 218.503 kb on - strandat 218.571 kb on + strandat 219.279 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml
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216,643 - Psyr_0201 0.57 +1.1
216,692 - Psyr_0201 0.59 -0.5
217,087 + Psyr_0201 0.78 -0.6
217,087 + Psyr_0201 0.78 -0.1
217,156 + Psyr_0201 0.82 -0.3
217,156 + Psyr_0201 0.82 -0.3
217,156 + Psyr_0201 0.82 -1.9
217,157 - Psyr_0201 0.82 -0.6
217,252 + Psyr_0201 0.86 -0.1
217,253 - Psyr_0201 0.86 +0.1
217,548 + -0.9
217,548 + +0.4
217,549 - +0.4
217,549 - +0.5
217,553 - +0.5
217,553 - +0.1
217,802 + Psyr_0202 0.28 +0.1
217,802 + Psyr_0202 0.28 -0.4
217,802 + Psyr_0202 0.28 -0.9
217,802 + Psyr_0202 0.28 -0.6
217,802 + Psyr_0202 0.28 -1.1
217,803 - Psyr_0202 0.28 -0.2
217,803 - Psyr_0202 0.28 -0.5
217,803 - Psyr_0202 0.28 -2.1
217,803 - Psyr_0202 0.28 +1.1
217,803 - Psyr_0202 0.28 -0.0
217,803 - Psyr_0202 0.28 +0.7
217,889 + Psyr_0202 0.37 -1.4
217,890 - Psyr_0202 0.37 -0.9
218,103 + Psyr_0202 0.60 +0.1
218,103 + Psyr_0202 0.60 +0.6
218,103 + Psyr_0202 0.60 -0.5
218,104 - Psyr_0202 0.60 +0.2
218,104 - Psyr_0202 0.60 -2.3
218,104 - Psyr_0202 0.60 -0.9
218,104 - Psyr_0202 0.60 -1.6
218,160 + Psyr_0202 0.66 -0.2
218,160 + Psyr_0202 0.66 -0.9
218,161 - Psyr_0202 0.66 -1.8
218,161 - Psyr_0202 0.66 -3.5
218,175 + Psyr_0202 0.68 -0.9
218,175 + Psyr_0202 0.68 -0.8
218,175 + Psyr_0202 0.68 -1.3
218,175 + Psyr_0202 0.68 -0.9
218,175 + Psyr_0202 0.68 +0.7
218,176 - Psyr_0202 0.68 -3.6
218,176 - Psyr_0202 0.68 -2.0
218,176 - Psyr_0202 0.68 -1.4
218,176 - Psyr_0202 0.68 -2.4
218,176 - Psyr_0202 0.68 -0.4
218,449 - -0.9
218,449 - -2.2
218,449 - -1.1
218,449 - -1.8
218,449 - -1.3
218,449 - -1.8
218,462 - -2.5
218,470 + -0.5
218,471 - -0.1
218,502 + +0.2
218,503 - -1.5
218,571 + -0.5
219,279 - +0.7

Or see this region's nucleotide sequence