Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0152 and Psyr_0153 overlap by 22 nucleotides Psyr_0153 and Psyr_0154 overlap by 4 nucleotides
Psyr_0152: Psyr_0152 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase:BFD-like [2Fe-2S]-binding region, at 165,081 to 166,490
_0152
Psyr_0153: Psyr_0153 - conserved hypothetical protein, at 166,469 to 166,780
_0153
Psyr_0154: Psyr_0154 - Oligopeptide/dipeptide ABC transporter, ATP-binding protein, C-terminal, at 166,777 to 168,618
_0154
Position (kb) 166
167 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 165.575 kb on + strand, within Psyr_0152 at 165.575 kb on + strand, within Psyr_0152 at 165.575 kb on + strand, within Psyr_0152 at 165.576 kb on - strand, within Psyr_0152 at 165.576 kb on - strand, within Psyr_0152 at 165.576 kb on - strand, within Psyr_0152 at 165.630 kb on + strand, within Psyr_0152 at 165.631 kb on - strand, within Psyr_0152 at 165.820 kb on + strand, within Psyr_0152 at 165.820 kb on + strand, within Psyr_0152 at 165.821 kb on - strand, within Psyr_0152 at 165.821 kb on - strand, within Psyr_0152 at 165.821 kb on - strand, within Psyr_0152 at 165.821 kb on - strand, within Psyr_0152 at 165.821 kb on - strand, within Psyr_0152 at 165.821 kb on - strand, within Psyr_0152 at 165.952 kb on + strand, within Psyr_0152 at 165.953 kb on - strand, within Psyr_0152 at 165.970 kb on + strand, within Psyr_0152 at 165.970 kb on + strand, within Psyr_0152 at 165.970 kb on + strand, within Psyr_0152 at 165.970 kb on + strand, within Psyr_0152 at 165.971 kb on - strand, within Psyr_0152 at 166.060 kb on + strand, within Psyr_0152 at 166.060 kb on + strand, within Psyr_0152 at 166.061 kb on - strand, within Psyr_0152 at 166.061 kb on - strand, within Psyr_0152 at 166.061 kb on - strand, within Psyr_0152 at 166.061 kb on - strand, within Psyr_0152 at 166.216 kb on + strand, within Psyr_0152 at 166.285 kb on + strand, within Psyr_0152 at 166.285 kb on + strand, within Psyr_0152 at 166.285 kb on + strand, within Psyr_0152 at 166.286 kb on - strand, within Psyr_0152 at 166.330 kb on + strand, within Psyr_0152 at 166.331 kb on - strand, within Psyr_0152 at 166.331 kb on - strand, within Psyr_0152 at 166.649 kb on + strand, within Psyr_0153 at 166.649 kb on + strand, within Psyr_0153 at 166.650 kb on - strand, within Psyr_0153 at 166.650 kb on - strand, within Psyr_0153 at 166.734 kb on - strand, within Psyr_0153 at 166.734 kb on - strand, within Psyr_0153 at 166.734 kb on - strand, within Psyr_0153 at 166.784 kb on + strand at 166.784 kb on + strand at 167.063 kb on + strand, within Psyr_0154 at 167.064 kb on - strand, within Psyr_0154 at 167.064 kb on - strand, within Psyr_0154 at 167.126 kb on + strand, within Psyr_0154 at 167.126 kb on + strand, within Psyr_0154 at 167.126 kb on + strand, within Psyr_0154 at 167.127 kb on - strand, within Psyr_0154 at 167.127 kb on - strand, within Psyr_0154 at 167.127 kb on - strand, within Psyr_0154 at 167.127 kb on - strand, within Psyr_0154 at 167.127 kb on - strand, within Psyr_0154 at 167.127 kb on - strand, within Psyr_0154 at 167.127 kb on - strand, within Psyr_0154 at 167.127 kb on - strand, within Psyr_0154 at 167.127 kb on - strand, within Psyr_0154 at 167.190 kb on - strand, within Psyr_0154 at 167.254 kb on - strand, within Psyr_0154 at 167.447 kb on + strand, within Psyr_0154 at 167.447 kb on + strand, within Psyr_0154 at 167.447 kb on + strand, within Psyr_0154 at 167.447 kb on + strand, within Psyr_0154 at 167.447 kb on + strand, within Psyr_0154 at 167.447 kb on + strand, within Psyr_0154 at 167.447 kb on + strand, within Psyr_0154 at 167.448 kb on - strand, within Psyr_0154 at 167.448 kb on - strand, within Psyr_0154 at 167.448 kb on - strand, within Psyr_0154 at 167.448 kb on - strand, within Psyr_0154 at 167.448 kb on - strand, within Psyr_0154
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 165,575 + Psyr_0152 0.35 -1.1 165,575 + Psyr_0152 0.35 -0.1 165,575 + Psyr_0152 0.35 -0.3 165,576 - Psyr_0152 0.35 -0.9 165,576 - Psyr_0152 0.35 -1.0 165,576 - Psyr_0152 0.35 -0.5 165,630 + Psyr_0152 0.39 +0.4 165,631 - Psyr_0152 0.39 -0.9 165,820 + Psyr_0152 0.52 +1.5 165,820 + Psyr_0152 0.52 -1.9 165,821 - Psyr_0152 0.52 +0.5 165,821 - Psyr_0152 0.52 +0.5 165,821 - Psyr_0152 0.52 -0.7 165,821 - Psyr_0152 0.52 +0.1 165,821 - Psyr_0152 0.52 -0.6 165,821 - Psyr_0152 0.52 +1.1 165,952 + Psyr_0152 0.62 -0.4 165,953 - Psyr_0152 0.62 -0.3 165,970 + Psyr_0152 0.63 -0.5 165,970 + Psyr_0152 0.63 -0.8 165,970 + Psyr_0152 0.63 +1.1 165,970 + Psyr_0152 0.63 -0.9 165,971 - Psyr_0152 0.63 -0.1 166,060 + Psyr_0152 0.69 -0.5 166,060 + Psyr_0152 0.69 -0.1 166,061 - Psyr_0152 0.70 -0.2 166,061 - Psyr_0152 0.70 -0.4 166,061 - Psyr_0152 0.70 +3.4 166,061 - Psyr_0152 0.70 +0.6 166,216 + Psyr_0152 0.80 -0.3 166,285 + Psyr_0152 0.85 -0.0 166,285 + Psyr_0152 0.85 +1.5 166,285 + Psyr_0152 0.85 +0.4 166,286 - Psyr_0152 0.85 +0.1 166,330 + Psyr_0152 0.89 +0.2 166,331 - Psyr_0152 0.89 +0.1 166,331 - Psyr_0152 0.89 +0.5 166,649 + Psyr_0153 0.58 -1.9 166,649 + Psyr_0153 0.58 -0.7 166,650 - Psyr_0153 0.58 -0.3 166,650 - Psyr_0153 0.58 -2.2 166,734 - Psyr_0153 0.85 -0.7 166,734 - Psyr_0153 0.85 -0.7 166,734 - Psyr_0153 0.85 +0.5 166,784 + +0.1 166,784 + +0.7 167,063 + Psyr_0154 0.16 -0.5 167,064 - Psyr_0154 0.16 -0.3 167,064 - Psyr_0154 0.16 -1.5 167,126 + Psyr_0154 0.19 -0.9 167,126 + Psyr_0154 0.19 -0.5 167,126 + Psyr_0154 0.19 +0.8 167,127 - Psyr_0154 0.19 -0.5 167,127 - Psyr_0154 0.19 -1.0 167,127 - Psyr_0154 0.19 -1.2 167,127 - Psyr_0154 0.19 +0.5 167,127 - Psyr_0154 0.19 +0.2 167,127 - Psyr_0154 0.19 +0.9 167,127 - Psyr_0154 0.19 +0.2 167,127 - Psyr_0154 0.19 -0.2 167,127 - Psyr_0154 0.19 -0.6 167,190 - Psyr_0154 0.22 +1.6 167,254 - Psyr_0154 0.26 -0.0 167,447 + Psyr_0154 0.36 -3.1 167,447 + Psyr_0154 0.36 -0.1 167,447 + Psyr_0154 0.36 -1.6 167,447 + Psyr_0154 0.36 +1.1 167,447 + Psyr_0154 0.36 +1.1 167,447 + Psyr_0154 0.36 +0.1 167,447 + Psyr_0154 0.36 +0.0 167,448 - Psyr_0154 0.36 -0.2 167,448 - Psyr_0154 0.36 -0.4 167,448 - Psyr_0154 0.36 +0.5 167,448 - Psyr_0154 0.36 -0.4 167,448 - Psyr_0154 0.36 -0.7
Or see this region's nucleotide sequence