Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5048 and Pf1N1B4_5049 are separated by 127 nucleotides Pf1N1B4_5049 and Pf1N1B4_5050 are separated by 12 nucleotides Pf1N1B4_5050 and Pf1N1B4_5051 are separated by 44 nucleotides Pf1N1B4_5051 and Pf1N1B4_5052 overlap by 4 nucleotides Pf1N1B4_5052 and Pf1N1B4_5053 overlap by 4 nucleotides
Pf1N1B4_5048 - Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), at 5,592,754 to 5,593,680
_5048
Pf1N1B4_5049 - FIG00959942: hypothetical protein, at 5,593,808 to 5,594,326
_5049
Pf1N1B4_5050 - hypothetical protein, at 5,594,339 to 5,594,452
_5050
Pf1N1B4_5051 - Sensor protein DegS, at 5,594,497 to 5,595,681
_5051
Pf1N1B4_5052 - Two-component response regulator, at 5,595,678 to 5,596,331
_5052
Pf1N1B4_5053 - Sensory box histidine kinase/response regulator, at 5,596,328 to 5,598,655
_5053
Position (kb)
5594
5595
5596 Strain fitness (log2 ratio)
-2
-1
0
1 at 5593.517 kb on - strand at 5593.588 kb on + strand at 5593.905 kb on + strand, within Pf1N1B4_5049 at 5594.055 kb on + strand, within Pf1N1B4_5049 at 5594.056 kb on - strand, within Pf1N1B4_5049 at 5594.056 kb on - strand, within Pf1N1B4_5049 at 5594.248 kb on - strand, within Pf1N1B4_5049 at 5594.248 kb on - strand, within Pf1N1B4_5049 at 5594.248 kb on - strand, within Pf1N1B4_5049 at 5594.248 kb on - strand, within Pf1N1B4_5049 at 5594.248 kb on - strand at 5594.248 kb on - strand, within Pf1N1B4_5049 at 5594.450 kb on + strand at 5594.451 kb on - strand at 5594.490 kb on + strand at 5594.753 kb on + strand, within Pf1N1B4_5051 at 5594.754 kb on - strand, within Pf1N1B4_5051 at 5594.886 kb on - strand, within Pf1N1B4_5051 at 5595.186 kb on - strand, within Pf1N1B4_5051 at 5595.650 kb on + strand at 5595.651 kb on - strand at 5595.651 kb on - strand at 5595.691 kb on + strand at 5596.368 kb on + strand at 5596.407 kb on + strand at 5596.436 kb on + strand at 5596.436 kb on + strand at 5596.476 kb on + strand at 5596.476 kb on + strand at 5596.476 kb on + strand at 5596.477 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 5,593,517 - +0.3 5,593,588 + +0.4 5,593,905 + Pf1N1B4_5049 0.19 -0.6 5,594,055 + Pf1N1B4_5049 0.48 +0.2 5,594,056 - Pf1N1B4_5049 0.48 +0.7 5,594,056 - Pf1N1B4_5049 0.48 -1.3 5,594,248 - Pf1N1B4_5049 0.85 +0.7 5,594,248 - Pf1N1B4_5049 0.85 -0.7 5,594,248 - Pf1N1B4_5049 0.85 -2.5 5,594,248 - Pf1N1B4_5049 0.85 -0.2 5,594,248 - +0.7 5,594,248 - Pf1N1B4_5049 0.85 -1.2 5,594,450 + -0.1 5,594,451 - -0.2 5,594,490 + -0.8 5,594,753 + Pf1N1B4_5051 0.22 -0.0 5,594,754 - Pf1N1B4_5051 0.22 -1.8 5,594,886 - Pf1N1B4_5051 0.33 -0.3 5,595,186 - Pf1N1B4_5051 0.58 +0.0 5,595,650 + -2.5 5,595,651 - +0.7 5,595,651 - +0.4 5,595,691 + +0.1 5,596,368 + -1.1 5,596,407 + +0.1 5,596,436 + -0.0 5,596,436 + +0.7 5,596,476 + -2.3 5,596,476 + +0.7 5,596,476 + -0.0 5,596,477 - +0.4
Or see this region's nucleotide sequence