Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5004

Experiment: copper (II) chloride 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5003 and Pf1N1B4_5004 are separated by 246 nucleotidesPf1N1B4_5004 and Pf1N1B4_5005 are separated by 285 nucleotides Pf1N1B4_5003 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 5,553,379 to 5,554,455 _5003 Pf1N1B4_5004 - Permease of the drug/metabolite transporter (DMT) superfamily, at 5,554,702 to 5,555,649 _5004 Pf1N1B4_5005 - LysR family transcriptional regulator STM2281, at 5,555,935 to 5,556,807 _5005 Position (kb) 5554 5555 5556Strain fitness (log2 ratio) -2 -1 0 1 2at 5553.867 kb on - strand, within Pf1N1B4_5003at 5553.921 kb on - strand, within Pf1N1B4_5003at 5553.936 kb on - strand, within Pf1N1B4_5003at 5554.043 kb on + strand, within Pf1N1B4_5003at 5554.043 kb on + strand, within Pf1N1B4_5003at 5554.043 kb on + strand, within Pf1N1B4_5003at 5554.043 kb on + strand, within Pf1N1B4_5003at 5554.079 kb on + strand, within Pf1N1B4_5003at 5554.079 kb on + strand, within Pf1N1B4_5003at 5554.080 kb on - strand, within Pf1N1B4_5003at 5554.181 kb on + strand, within Pf1N1B4_5003at 5554.570 kb on - strandat 5554.570 kb on - strandat 5554.654 kb on - strandat 5554.654 kb on - strandat 5554.654 kb on - strandat 5554.656 kb on - strandat 5554.901 kb on + strand, within Pf1N1B4_5004at 5554.913 kb on + strand, within Pf1N1B4_5004at 5554.913 kb on + strand, within Pf1N1B4_5004at 5554.913 kb on + strandat 5554.914 kb on - strand, within Pf1N1B4_5004at 5554.914 kb on - strand, within Pf1N1B4_5004at 5554.914 kb on - strand, within Pf1N1B4_5004at 5554.914 kb on - strandat 5555.112 kb on - strand, within Pf1N1B4_5004at 5555.348 kb on + strandat 5555.348 kb on + strand, within Pf1N1B4_5004at 5555.348 kb on + strandat 5555.349 kb on - strand, within Pf1N1B4_5004at 5555.349 kb on - strand, within Pf1N1B4_5004at 5555.349 kb on - strandat 5555.360 kb on + strand, within Pf1N1B4_5004at 5555.378 kb on + strand, within Pf1N1B4_5004at 5555.379 kb on - strand, within Pf1N1B4_5004at 5555.379 kb on - strand, within Pf1N1B4_5004at 5555.639 kb on + strandat 5555.640 kb on - strandat 5555.643 kb on + strandat 5555.643 kb on + strandat 5555.643 kb on + strandat 5555.644 kb on - strandat 5556.068 kb on + strandat 5556.068 kb on + strand, within Pf1N1B4_5005at 5556.129 kb on - strand, within Pf1N1B4_5005at 5556.358 kb on - strand, within Pf1N1B4_5005at 5556.488 kb on + strandat 5556.507 kb on - strandat 5556.549 kb on + strand, within Pf1N1B4_5005at 5556.549 kb on + strand, within Pf1N1B4_5005

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM
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5,553,867 - Pf1N1B4_5003 0.45 +0.4
5,553,921 - Pf1N1B4_5003 0.50 -2.6
5,553,936 - Pf1N1B4_5003 0.52 -2.1
5,554,043 + Pf1N1B4_5003 0.62 -0.3
5,554,043 + Pf1N1B4_5003 0.62 +0.1
5,554,043 + Pf1N1B4_5003 0.62 -0.5
5,554,043 + Pf1N1B4_5003 0.62 -0.6
5,554,079 + Pf1N1B4_5003 0.65 -0.6
5,554,079 + Pf1N1B4_5003 0.65 -0.3
5,554,080 - Pf1N1B4_5003 0.65 -1.9
5,554,181 + Pf1N1B4_5003 0.74 -1.6
5,554,570 - -1.0
5,554,570 - -2.1
5,554,654 - +0.1
5,554,654 - -1.5
5,554,654 - -1.1
5,554,656 - -0.9
5,554,901 + Pf1N1B4_5004 0.21 -1.1
5,554,913 + Pf1N1B4_5004 0.22 -0.6
5,554,913 + Pf1N1B4_5004 0.22 -0.7
5,554,913 + +0.7
5,554,914 - Pf1N1B4_5004 0.22 -2.1
5,554,914 - Pf1N1B4_5004 0.22 -0.3
5,554,914 - Pf1N1B4_5004 0.22 -0.6
5,554,914 - -0.3
5,555,112 - Pf1N1B4_5004 0.43 -0.0
5,555,348 + -0.3
5,555,348 + Pf1N1B4_5004 0.68 -2.1
5,555,348 + -0.3
5,555,349 - Pf1N1B4_5004 0.68 +1.0
5,555,349 - Pf1N1B4_5004 0.68 -1.6
5,555,349 - +1.7
5,555,360 + Pf1N1B4_5004 0.69 +0.1
5,555,378 + Pf1N1B4_5004 0.71 -1.7
5,555,379 - Pf1N1B4_5004 0.71 -1.6
5,555,379 - Pf1N1B4_5004 0.71 -2.7
5,555,639 + +0.5
5,555,640 - +0.7
5,555,643 + -0.7
5,555,643 + -0.0
5,555,643 + +0.1
5,555,644 - -1.7
5,556,068 + -1.6
5,556,068 + Pf1N1B4_5005 0.15 -0.8
5,556,129 - Pf1N1B4_5005 0.22 -0.0
5,556,358 - Pf1N1B4_5005 0.48 -0.3
5,556,488 + +2.3
5,556,507 - -0.3
5,556,549 + Pf1N1B4_5005 0.70 -1.8
5,556,549 + Pf1N1B4_5005 0.70 -0.0

Or see this region's nucleotide sequence