Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_372 and Pf1N1B4_373 are separated by 323 nucleotides Pf1N1B4_373 and Pf1N1B4_374 are separated by 123 nucleotides
Pf1N1B4_372 - UDP-galactose-lipid carrier transferase (EC 2.-.-.-), at 439,678 to 441,201
_372
Pf1N1B4_373 - 5-methylaminomethyl-2-thiouridine-forming enzyme mnmC, at 441,525 to 443,507
_373
Pf1N1B4_374 - Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4), at 443,631 to 445,403
_374
Position (kb) 441
442
443
444 Strain fitness (log2 ratio) -2
-1
0
1
2 at 440.547 kb on + strand, within Pf1N1B4_372 at 440.549 kb on + strand, within Pf1N1B4_372 at 440.550 kb on - strand, within Pf1N1B4_372 at 440.624 kb on + strand, within Pf1N1B4_372 at 440.624 kb on + strand at 440.625 kb on - strand, within Pf1N1B4_372 at 440.643 kb on - strand, within Pf1N1B4_372 at 441.155 kb on + strand at 441.155 kb on + strand at 441.155 kb on + strand at 441.156 kb on - strand at 441.156 kb on - strand at 441.156 kb on - strand at 441.201 kb on + strand at 441.201 kb on + strand at 441.202 kb on - strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.254 kb on + strand at 441.254 kb on + strand at 441.254 kb on + strand at 441.255 kb on - strand at 441.255 kb on - strand at 441.255 kb on - strand at 441.255 kb on - strand at 441.360 kb on + strand at 441.360 kb on + strand at 441.361 kb on - strand at 441.361 kb on - strand at 441.361 kb on - strand at 441.361 kb on - strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.900 kb on + strand, within Pf1N1B4_373 at 442.203 kb on - strand, within Pf1N1B4_373 at 442.449 kb on + strand at 442.449 kb on + strand, within Pf1N1B4_373 at 442.711 kb on - strand, within Pf1N1B4_373 at 443.211 kb on - strand, within Pf1N1B4_373 at 443.211 kb on - strand, within Pf1N1B4_373 at 443.275 kb on - strand, within Pf1N1B4_373 at 443.518 kb on + strand at 443.518 kb on + strand at 443.518 kb on + strand at 443.519 kb on - strand at 443.519 kb on - strand at 443.520 kb on + strand at 443.520 kb on + strand at 443.520 kb on + strand at 443.521 kb on - strand at 443.590 kb on + strand at 443.630 kb on + strand at 443.630 kb on + strand at 443.630 kb on + strand at 443.630 kb on + strand at 443.630 kb on + strand at 443.631 kb on - strand at 443.631 kb on - strand at 443.631 kb on - strand at 443.631 kb on - strand at 443.631 kb on - strand at 443.631 kb on - strand at 443.631 kb on - strand at 443.641 kb on + strand at 443.654 kb on - strand at 443.654 kb on - strand at 443.865 kb on + strand, within Pf1N1B4_374 at 443.866 kb on - strand, within Pf1N1B4_374 at 443.866 kb on - strand, within Pf1N1B4_374 at 443.916 kb on + strand at 443.917 kb on - strand, within Pf1N1B4_374 at 443.917 kb on - strand, within Pf1N1B4_374 at 443.953 kb on - strand, within Pf1N1B4_374 at 444.072 kb on + strand, within Pf1N1B4_374 at 444.072 kb on + strand, within Pf1N1B4_374 at 444.072 kb on + strand, within Pf1N1B4_374 at 444.073 kb on - strand, within Pf1N1B4_374 at 444.073 kb on - strand, within Pf1N1B4_374 at 444.073 kb on - strand, within Pf1N1B4_374 at 444.177 kb on + strand, within Pf1N1B4_374 at 444.460 kb on + strand, within Pf1N1B4_374 at 444.460 kb on + strand, within Pf1N1B4_374
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 440,547 + Pf1N1B4_372 0.57 +0.3 440,549 + Pf1N1B4_372 0.57 +0.1 440,550 - Pf1N1B4_372 0.57 +0.1 440,624 + Pf1N1B4_372 0.62 +0.1 440,624 + +0.7 440,625 - Pf1N1B4_372 0.62 -0.7 440,643 - Pf1N1B4_372 0.63 -0.7 441,155 + -0.4 441,155 + +0.3 441,155 + -0.7 441,156 - +0.4 441,156 - -1.0 441,156 - -0.6 441,201 + +0.6 441,201 + +1.1 441,202 - -0.3 441,250 + -1.2 441,250 + -1.4 441,250 + +1.9 441,250 + +0.7 441,250 + +0.1 441,250 + -0.4 441,250 + +1.1 441,250 + -0.1 441,250 + +0.1 441,251 - -0.9 441,251 - -1.0 441,251 - -2.1 441,251 - -0.4 441,251 - +0.4 441,254 + -0.3 441,254 + -0.4 441,254 + -0.6 441,255 - +1.9 441,255 - +1.1 441,255 - -0.4 441,255 - -0.9 441,360 + -1.1 441,360 + -2.4 441,361 - +0.8 441,361 - +0.1 441,361 - -0.4 441,361 - +0.4 441,364 + -1.0 441,364 + -1.2 441,364 + -1.3 441,364 + -1.3 441,364 + +2.1 441,364 + +1.1 441,365 - +0.4 441,365 - -0.7 441,365 - -0.9 441,365 - +1.1 441,365 - +0.1 441,365 - +1.1 441,603 + -0.9 441,603 + +1.0 441,603 + +0.4 441,603 + -0.4 441,603 + -0.6 441,900 + Pf1N1B4_373 0.19 -1.9 442,203 - Pf1N1B4_373 0.34 -0.4 442,449 + +2.7 442,449 + Pf1N1B4_373 0.47 -0.6 442,711 - Pf1N1B4_373 0.60 +1.1 443,211 - Pf1N1B4_373 0.85 -1.8 443,211 - Pf1N1B4_373 0.85 -0.4 443,275 - Pf1N1B4_373 0.88 -0.4 443,518 + +0.1 443,518 + -0.8 443,518 + -0.2 443,519 - +2.0 443,519 - +0.4 443,520 + +0.7 443,520 + -2.2 443,520 + +0.1 443,521 - -1.9 443,590 + -1.0 443,630 + +0.3 443,630 + -0.7 443,630 + -0.9 443,630 + -0.9 443,630 + -1.2 443,631 - +0.7 443,631 - -0.3 443,631 - +0.0 443,631 - -0.1 443,631 - +0.1 443,631 - -0.9 443,631 - +2.1 443,641 + +2.7 443,654 - +0.4 443,654 - -0.0 443,865 + Pf1N1B4_374 0.13 -1.9 443,866 - Pf1N1B4_374 0.13 -0.9 443,866 - Pf1N1B4_374 0.13 -0.4 443,916 + -1.4 443,917 - Pf1N1B4_374 0.16 +0.5 443,917 - Pf1N1B4_374 0.16 +0.1 443,953 - Pf1N1B4_374 0.18 -0.4 444,072 + Pf1N1B4_374 0.25 +0.1 444,072 + Pf1N1B4_374 0.25 +0.1 444,072 + Pf1N1B4_374 0.25 -0.2 444,073 - Pf1N1B4_374 0.25 -0.9 444,073 - Pf1N1B4_374 0.25 -0.7 444,073 - Pf1N1B4_374 0.25 -0.4 444,177 + Pf1N1B4_374 0.31 +0.4 444,460 + Pf1N1B4_374 0.47 -0.3 444,460 + Pf1N1B4_374 0.47 +2.7
Or see this region's nucleotide sequence