Experiment: R2A with Paraquat dichloride 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25385 and CA265_RS25390 are separated by 16 nucleotides CA265_RS25390 and CA265_RS25395 are separated by 31 nucleotides CA265_RS25395 and CA265_RS25400 are separated by 81 nucleotides CA265_RS25400 and CA265_RS25405 are separated by 260 nucleotides
CA265_RS25385: CA265_RS25385 - Txe/YoeB family addiction module toxin, at 6,167,020 to 6,167,301
_RS25385
CA265_RS25390: CA265_RS25390 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 6,167,318 to 6,168,169
_RS25390
CA265_RS25395: CA265_RS25395 - cysteine hydrolase, at 6,168,201 to 6,168,743
_RS25395
CA265_RS25400: CA265_RS25400 - fatty acid desaturase, at 6,168,825 to 6,169,451
_RS25400
CA265_RS25405: CA265_RS25405 - ferrochelatase, at 6,169,712 to 6,170,722
_RS25405
Position (kb)
6168
6169 Strain fitness (log2 ratio)
-1
0
1 at 6167.231 kb on + strand, within CA265_RS25385 at 6167.231 kb on + strand, within CA265_RS25385 at 6167.233 kb on + strand, within CA265_RS25385 at 6167.256 kb on + strand, within CA265_RS25385 at 6168.186 kb on - strand at 6168.188 kb on - strand at 6168.191 kb on + strand at 6168.220 kb on + strand at 6168.290 kb on - strand, within CA265_RS25395 at 6168.294 kb on - strand, within CA265_RS25395 at 6168.450 kb on - strand, within CA265_RS25395 at 6168.484 kb on + strand, within CA265_RS25395 at 6168.484 kb on + strand, within CA265_RS25395 at 6168.579 kb on + strand, within CA265_RS25395 at 6168.631 kb on + strand, within CA265_RS25395 at 6168.646 kb on + strand, within CA265_RS25395 at 6168.710 kb on - strand at 6168.717 kb on - strand at 6168.742 kb on - strand at 6168.786 kb on - strand at 6168.786 kb on - strand at 6168.787 kb on + strand at 6168.795 kb on + strand at 6168.795 kb on + strand at 6168.796 kb on - strand at 6168.818 kb on - strand at 6168.824 kb on - strand at 6168.861 kb on - strand at 6168.861 kb on - strand at 6168.894 kb on + strand, within CA265_RS25400 at 6169.081 kb on + strand, within CA265_RS25400 at 6169.132 kb on - strand, within CA265_RS25400 at 6169.153 kb on - strand, within CA265_RS25400 at 6169.191 kb on + strand, within CA265_RS25400 at 6169.191 kb on + strand, within CA265_RS25400 at 6169.192 kb on - strand, within CA265_RS25400 at 6169.192 kb on - strand, within CA265_RS25400 at 6169.192 kb on - strand, within CA265_RS25400 at 6169.255 kb on + strand, within CA265_RS25400 at 6169.290 kb on + strand, within CA265_RS25400 at 6169.368 kb on - strand, within CA265_RS25400 at 6169.375 kb on - strand, within CA265_RS25400 at 6169.387 kb on - strand, within CA265_RS25400 at 6169.415 kb on + strand at 6169.416 kb on - strand at 6169.541 kb on - strand at 6169.541 kb on - strand at 6169.651 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.003 mg/ml remove 6,167,231 + CA265_RS25385 0.75 -0.0 6,167,231 + CA265_RS25385 0.75 -0.3 6,167,233 + CA265_RS25385 0.76 +1.1 6,167,256 + CA265_RS25385 0.84 -0.1 6,168,186 - +1.1 6,168,188 - +0.0 6,168,191 + -0.5 6,168,220 + +0.4 6,168,290 - CA265_RS25395 0.16 -0.5 6,168,294 - CA265_RS25395 0.17 -0.2 6,168,450 - CA265_RS25395 0.46 -1.1 6,168,484 + CA265_RS25395 0.52 +0.3 6,168,484 + CA265_RS25395 0.52 -0.2 6,168,579 + CA265_RS25395 0.70 -0.5 6,168,631 + CA265_RS25395 0.79 +0.1 6,168,646 + CA265_RS25395 0.82 -1.0 6,168,710 - -0.2 6,168,717 - +1.6 6,168,742 - -0.1 6,168,786 - +0.6 6,168,786 - +0.1 6,168,787 + -0.7 6,168,795 + +0.6 6,168,795 + -0.4 6,168,796 - +1.4 6,168,818 - -1.5 6,168,824 - -0.3 6,168,861 - -0.3 6,168,861 - -0.4 6,168,894 + CA265_RS25400 0.11 -1.7 6,169,081 + CA265_RS25400 0.41 -0.2 6,169,132 - CA265_RS25400 0.49 -0.5 6,169,153 - CA265_RS25400 0.52 -0.6 6,169,191 + CA265_RS25400 0.58 -0.1 6,169,191 + CA265_RS25400 0.58 +0.2 6,169,192 - CA265_RS25400 0.59 -0.4 6,169,192 - CA265_RS25400 0.59 -0.5 6,169,192 - CA265_RS25400 0.59 +0.3 6,169,255 + CA265_RS25400 0.69 -0.6 6,169,290 + CA265_RS25400 0.74 +0.7 6,169,368 - CA265_RS25400 0.87 +0.6 6,169,375 - CA265_RS25400 0.88 -0.0 6,169,387 - CA265_RS25400 0.90 -0.7 6,169,415 + -0.4 6,169,416 - +0.4 6,169,541 - -0.4 6,169,541 - +0.6 6,169,651 + +0.7
Or see this region's nucleotide sequence