Experiment: R2A with Paraquat dichloride 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15490 and CA265_RS15495 are separated by 25 nucleotides CA265_RS15495 and CA265_RS15500 are separated by 12 nucleotides CA265_RS15500 and CA265_RS15505 are separated by 47 nucleotides
CA265_RS15490: CA265_RS15490 - sulfatase, at 3,894,465 to 3,896,351
_RS15490
CA265_RS15495: CA265_RS15495 - hypothetical protein, at 3,896,377 to 3,897,096
_RS15495
CA265_RS15500: CA265_RS15500 - hypothetical protein, at 3,897,109 to 3,897,522
_RS15500
CA265_RS15505: CA265_RS15505 - RagB/SusD family nutrient uptake outer membrane protein, at 3,897,570 to 3,899,180
_RS15505
Position (kb)
3897
3898 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3896.119 kb on - strand, within CA265_RS15490 at 3896.250 kb on + strand at 3896.283 kb on - strand at 3896.303 kb on + strand at 3896.303 kb on + strand at 3896.307 kb on + strand at 3896.310 kb on - strand at 3896.378 kb on + strand at 3896.500 kb on - strand, within CA265_RS15495 at 3896.637 kb on - strand, within CA265_RS15495 at 3896.663 kb on - strand, within CA265_RS15495 at 3896.689 kb on - strand, within CA265_RS15495 at 3896.806 kb on + strand, within CA265_RS15495 at 3896.806 kb on + strand, within CA265_RS15495 at 3896.814 kb on + strand, within CA265_RS15495 at 3896.814 kb on + strand, within CA265_RS15495 at 3896.815 kb on - strand, within CA265_RS15495 at 3896.815 kb on - strand, within CA265_RS15495 at 3896.849 kb on + strand, within CA265_RS15495 at 3896.854 kb on - strand, within CA265_RS15495 at 3896.854 kb on - strand, within CA265_RS15495 at 3896.860 kb on - strand, within CA265_RS15495 at 3897.031 kb on - strand at 3897.053 kb on - strand at 3897.053 kb on - strand at 3897.053 kb on - strand at 3897.054 kb on + strand at 3897.054 kb on + strand at 3897.055 kb on - strand at 3897.056 kb on + strand at 3897.057 kb on - strand at 3897.169 kb on - strand, within CA265_RS15500 at 3897.169 kb on - strand, within CA265_RS15500 at 3897.214 kb on - strand, within CA265_RS15500 at 3897.348 kb on + strand, within CA265_RS15500 at 3897.348 kb on + strand, within CA265_RS15500 at 3897.348 kb on + strand, within CA265_RS15500 at 3897.348 kb on + strand, within CA265_RS15500 at 3897.362 kb on + strand, within CA265_RS15500 at 3897.484 kb on + strand at 3897.484 kb on + strand at 3897.535 kb on - strand at 3897.542 kb on - strand at 3897.563 kb on + strand at 3897.563 kb on + strand at 3897.631 kb on + strand at 3897.632 kb on - strand at 3897.749 kb on - strand, within CA265_RS15505 at 3897.749 kb on - strand, within CA265_RS15505 at 3897.753 kb on + strand, within CA265_RS15505 at 3897.754 kb on - strand, within CA265_RS15505 at 3897.788 kb on + strand, within CA265_RS15505 at 3897.789 kb on - strand, within CA265_RS15505 at 3897.934 kb on + strand, within CA265_RS15505 at 3897.977 kb on + strand, within CA265_RS15505 at 3898.029 kb on - strand, within CA265_RS15505 at 3898.051 kb on + strand, within CA265_RS15505 at 3898.074 kb on - strand, within CA265_RS15505 at 3898.078 kb on - strand, within CA265_RS15505 at 3898.078 kb on - strand, within CA265_RS15505 at 3898.078 kb on - strand, within CA265_RS15505 at 3898.119 kb on + strand, within CA265_RS15505 at 3898.156 kb on + strand, within CA265_RS15505 at 3898.157 kb on - strand, within CA265_RS15505 at 3898.158 kb on + strand, within CA265_RS15505 at 3898.178 kb on - strand, within CA265_RS15505 at 3898.196 kb on + strand, within CA265_RS15505 at 3898.198 kb on + strand, within CA265_RS15505 at 3898.220 kb on - strand, within CA265_RS15505 at 3898.220 kb on - strand, within CA265_RS15505 at 3898.240 kb on - strand, within CA265_RS15505 at 3898.249 kb on - strand, within CA265_RS15505 at 3898.292 kb on + strand at 3898.314 kb on + strand, within CA265_RS15505 at 3898.360 kb on + strand, within CA265_RS15505 at 3898.360 kb on + strand, within CA265_RS15505 at 3898.365 kb on - strand, within CA265_RS15505
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.003 mg/ml remove 3,896,119 - CA265_RS15490 0.88 +1.3 3,896,250 + -1.0 3,896,283 - -0.8 3,896,303 + +0.9 3,896,303 + -0.3 3,896,307 + +0.3 3,896,310 - -0.5 3,896,378 + -0.3 3,896,500 - CA265_RS15495 0.17 -1.4 3,896,637 - CA265_RS15495 0.36 -0.2 3,896,663 - CA265_RS15495 0.40 +2.5 3,896,689 - CA265_RS15495 0.43 -0.9 3,896,806 + CA265_RS15495 0.60 +0.7 3,896,806 + CA265_RS15495 0.60 -1.2 3,896,814 + CA265_RS15495 0.61 -1.9 3,896,814 + CA265_RS15495 0.61 -0.6 3,896,815 - CA265_RS15495 0.61 -0.7 3,896,815 - CA265_RS15495 0.61 -0.6 3,896,849 + CA265_RS15495 0.66 +0.9 3,896,854 - CA265_RS15495 0.66 -0.1 3,896,854 - CA265_RS15495 0.66 -0.2 3,896,860 - CA265_RS15495 0.67 -0.4 3,897,031 - -0.1 3,897,053 - -0.8 3,897,053 - +0.0 3,897,053 - -0.4 3,897,054 + -0.3 3,897,054 + +0.3 3,897,055 - -0.8 3,897,056 + -2.0 3,897,057 - +0.1 3,897,169 - CA265_RS15500 0.14 +0.4 3,897,169 - CA265_RS15500 0.14 -0.0 3,897,214 - CA265_RS15500 0.25 -0.5 3,897,348 + CA265_RS15500 0.58 -0.3 3,897,348 + CA265_RS15500 0.58 -0.8 3,897,348 + CA265_RS15500 0.58 -0.1 3,897,348 + CA265_RS15500 0.58 -0.6 3,897,362 + CA265_RS15500 0.61 -0.3 3,897,484 + -1.7 3,897,484 + -1.9 3,897,535 - -0.7 3,897,542 - +0.1 3,897,563 + -0.1 3,897,563 + -0.7 3,897,631 + +0.4 3,897,632 - -0.9 3,897,749 - CA265_RS15505 0.11 -1.8 3,897,749 - CA265_RS15505 0.11 +0.5 3,897,753 + CA265_RS15505 0.11 -0.5 3,897,754 - CA265_RS15505 0.11 +0.6 3,897,788 + CA265_RS15505 0.14 -0.7 3,897,789 - CA265_RS15505 0.14 -0.3 3,897,934 + CA265_RS15505 0.23 +0.4 3,897,977 + CA265_RS15505 0.25 +1.0 3,898,029 - CA265_RS15505 0.28 -1.4 3,898,051 + CA265_RS15505 0.30 +0.8 3,898,074 - CA265_RS15505 0.31 -0.1 3,898,078 - CA265_RS15505 0.32 -1.7 3,898,078 - CA265_RS15505 0.32 -0.7 3,898,078 - CA265_RS15505 0.32 +0.3 3,898,119 + CA265_RS15505 0.34 +1.5 3,898,156 + CA265_RS15505 0.36 +0.0 3,898,157 - CA265_RS15505 0.36 -0.3 3,898,158 + CA265_RS15505 0.36 -0.2 3,898,178 - CA265_RS15505 0.38 -0.3 3,898,196 + CA265_RS15505 0.39 +0.0 3,898,198 + CA265_RS15505 0.39 +0.9 3,898,220 - CA265_RS15505 0.40 -0.4 3,898,220 - CA265_RS15505 0.40 -1.5 3,898,240 - CA265_RS15505 0.42 -0.8 3,898,249 - CA265_RS15505 0.42 -0.3 3,898,292 + +1.9 3,898,314 + CA265_RS15505 0.46 +0.3 3,898,360 + CA265_RS15505 0.49 +0.2 3,898,360 + CA265_RS15505 0.49 +1.8 3,898,365 - CA265_RS15505 0.49 -1.8
Or see this region's nucleotide sequence