Experiment: R2A with Paraquat dichloride 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10585 and CA265_RS10590 are separated by 198 nucleotides CA265_RS10590 and CA265_RS10595 are separated by 88 nucleotides CA265_RS10595 and CA265_RS10600 are separated by 160 nucleotides CA265_RS10600 and CA265_RS10605 overlap by 14 nucleotides
CA265_RS10585: CA265_RS10585 - transcriptional regulator, at 2,619,819 to 2,620,181
_RS10585
CA265_RS10590: CA265_RS10590 - hypothetical protein, at 2,620,380 to 2,620,880
_RS10590
CA265_RS10595: CA265_RS10595 - dicarboxylate/amino acid:cation symporter, at 2,620,969 to 2,622,345
_RS10595
CA265_RS10600: CA265_RS10600 - RNA polymerase subunit sigma-24, at 2,622,506 to 2,623,003
_RS10600
CA265_RS10605: CA265_RS10605 - hypothetical protein, at 2,622,990 to 2,623,589
_RS10605
Position (kb)
2620
2621
2622
2623 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2619.991 kb on + strand, within CA265_RS10585 at 2620.056 kb on - strand, within CA265_RS10585 at 2620.068 kb on - strand, within CA265_RS10585 at 2620.095 kb on + strand, within CA265_RS10585 at 2620.103 kb on + strand, within CA265_RS10585 at 2620.115 kb on + strand, within CA265_RS10585 at 2620.124 kb on + strand, within CA265_RS10585 at 2620.124 kb on + strand, within CA265_RS10585 at 2620.128 kb on + strand, within CA265_RS10585 at 2620.259 kb on + strand at 2620.260 kb on - strand at 2620.294 kb on - strand at 2620.747 kb on + strand, within CA265_RS10590 at 2620.811 kb on + strand at 2621.002 kb on + strand at 2621.002 kb on + strand at 2621.227 kb on + strand, within CA265_RS10595 at 2621.227 kb on + strand, within CA265_RS10595 at 2621.228 kb on - strand, within CA265_RS10595 at 2621.228 kb on - strand, within CA265_RS10595 at 2621.228 kb on - strand, within CA265_RS10595 at 2621.228 kb on - strand, within CA265_RS10595 at 2621.288 kb on - strand, within CA265_RS10595 at 2621.353 kb on + strand, within CA265_RS10595 at 2621.354 kb on - strand, within CA265_RS10595 at 2621.398 kb on - strand, within CA265_RS10595 at 2621.530 kb on + strand, within CA265_RS10595 at 2621.532 kb on + strand, within CA265_RS10595 at 2621.533 kb on - strand, within CA265_RS10595 at 2621.538 kb on - strand, within CA265_RS10595 at 2621.546 kb on - strand, within CA265_RS10595 at 2621.549 kb on - strand, within CA265_RS10595 at 2621.555 kb on - strand, within CA265_RS10595 at 2621.621 kb on + strand, within CA265_RS10595 at 2621.622 kb on - strand, within CA265_RS10595 at 2621.684 kb on - strand, within CA265_RS10595 at 2621.855 kb on - strand, within CA265_RS10595 at 2621.979 kb on + strand, within CA265_RS10595 at 2622.049 kb on + strand, within CA265_RS10595 at 2622.163 kb on - strand, within CA265_RS10595 at 2622.239 kb on - strand at 2622.239 kb on - strand at 2622.241 kb on - strand at 2622.385 kb on - strand at 2622.390 kb on - strand at 2622.482 kb on + strand at 2622.493 kb on + strand at 2622.493 kb on + strand at 2622.495 kb on + strand at 2622.495 kb on + strand at 2622.525 kb on + strand at 2622.525 kb on + strand at 2622.570 kb on + strand, within CA265_RS10600 at 2622.581 kb on + strand, within CA265_RS10600 at 2622.583 kb on + strand, within CA265_RS10600 at 2622.715 kb on - strand, within CA265_RS10600 at 2622.719 kb on + strand, within CA265_RS10600 at 2622.719 kb on + strand, within CA265_RS10600 at 2622.756 kb on + strand, within CA265_RS10600 at 2622.763 kb on + strand, within CA265_RS10600 at 2622.783 kb on + strand, within CA265_RS10600 at 2622.826 kb on + strand, within CA265_RS10600 at 2622.869 kb on - strand, within CA265_RS10600 at 2622.893 kb on + strand, within CA265_RS10600 at 2622.959 kb on - strand at 2622.959 kb on - strand at 2622.978 kb on + strand at 2622.979 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.003 mg/ml remove 2,619,991 + CA265_RS10585 0.47 -0.3 2,620,056 - CA265_RS10585 0.65 +0.3 2,620,068 - CA265_RS10585 0.69 +0.4 2,620,095 + CA265_RS10585 0.76 +0.1 2,620,103 + CA265_RS10585 0.78 -1.0 2,620,115 + CA265_RS10585 0.82 +1.5 2,620,124 + CA265_RS10585 0.84 +0.1 2,620,124 + CA265_RS10585 0.84 -0.0 2,620,128 + CA265_RS10585 0.85 +0.4 2,620,259 + -0.6 2,620,260 - -0.6 2,620,294 - -0.2 2,620,747 + CA265_RS10590 0.73 +1.2 2,620,811 + +0.6 2,621,002 + +2.1 2,621,002 + +0.9 2,621,227 + CA265_RS10595 0.19 +1.4 2,621,227 + CA265_RS10595 0.19 +1.9 2,621,228 - CA265_RS10595 0.19 +3.1 2,621,228 - CA265_RS10595 0.19 +2.7 2,621,228 - CA265_RS10595 0.19 +4.3 2,621,228 - CA265_RS10595 0.19 +4.3 2,621,288 - CA265_RS10595 0.23 +3.5 2,621,353 + CA265_RS10595 0.28 +0.6 2,621,354 - CA265_RS10595 0.28 +3.2 2,621,398 - CA265_RS10595 0.31 +4.1 2,621,530 + CA265_RS10595 0.41 +1.2 2,621,532 + CA265_RS10595 0.41 +0.1 2,621,533 - CA265_RS10595 0.41 +3.4 2,621,538 - CA265_RS10595 0.41 +2.9 2,621,546 - CA265_RS10595 0.42 +3.4 2,621,549 - CA265_RS10595 0.42 +3.2 2,621,555 - CA265_RS10595 0.43 +2.7 2,621,621 + CA265_RS10595 0.47 +1.9 2,621,622 - CA265_RS10595 0.47 +3.0 2,621,684 - CA265_RS10595 0.52 +3.1 2,621,855 - CA265_RS10595 0.64 +3.4 2,621,979 + CA265_RS10595 0.73 +1.1 2,622,049 + CA265_RS10595 0.78 +2.0 2,622,163 - CA265_RS10595 0.87 +2.0 2,622,239 - +2.9 2,622,239 - +1.8 2,622,241 - +2.3 2,622,385 - -1.8 2,622,390 - +0.1 2,622,482 + -1.1 2,622,493 + -0.5 2,622,493 + -0.1 2,622,495 + -0.9 2,622,495 + -0.9 2,622,525 + -0.8 2,622,525 + -0.6 2,622,570 + CA265_RS10600 0.13 +0.6 2,622,581 + CA265_RS10600 0.15 +0.4 2,622,583 + CA265_RS10600 0.15 -1.7 2,622,715 - CA265_RS10600 0.42 -1.9 2,622,719 + CA265_RS10600 0.43 -0.2 2,622,719 + CA265_RS10600 0.43 -2.1 2,622,756 + CA265_RS10600 0.50 -1.3 2,622,763 + CA265_RS10600 0.52 -2.0 2,622,783 + CA265_RS10600 0.56 -0.3 2,622,826 + CA265_RS10600 0.64 -0.2 2,622,869 - CA265_RS10600 0.73 -2.3 2,622,893 + CA265_RS10600 0.78 -2.0 2,622,959 - -0.6 2,622,959 - -0.9 2,622,978 + +4.9 2,622,979 - -2.0
Or see this region's nucleotide sequence