Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29745 and BPHYT_RS29750 overlap by 4 nucleotides BPHYT_RS29750 and BPHYT_RS29755 are separated by 603 nucleotides
BPHYT_RS29745: BPHYT_RS29745 - phosphonopyruvate decarboxylase, at 2,226,021 to 2,227,184
_RS29745
BPHYT_RS29750: BPHYT_RS29750 - phosphoenolpyruvate phosphomutase, at 2,227,181 to 2,228,926
_RS29750
BPHYT_RS29755: BPHYT_RS29755 - membrane protein, at 2,229,530 to 2,231,071
_RS29755
Position (kb)
2227
2228
2229 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2226.460 kb on + strand, within BPHYT_RS29745 at 2226.673 kb on + strand, within BPHYT_RS29745 at 2227.061 kb on - strand, within BPHYT_RS29745 at 2227.087 kb on + strand at 2227.087 kb on + strand at 2227.339 kb on - strand at 2227.564 kb on + strand, within BPHYT_RS29750 at 2227.565 kb on - strand, within BPHYT_RS29750 at 2227.867 kb on + strand, within BPHYT_RS29750 at 2227.867 kb on + strand, within BPHYT_RS29750 at 2228.250 kb on + strand, within BPHYT_RS29750 at 2228.749 kb on - strand, within BPHYT_RS29750 at 2228.749 kb on - strand, within BPHYT_RS29750 at 2228.929 kb on + strand at 2228.929 kb on + strand at 2228.933 kb on + strand at 2228.934 kb on - strand at 2228.934 kb on - strand at 2228.965 kb on + strand at 2228.966 kb on - strand at 2229.185 kb on - strand at 2229.451 kb on + strand at 2229.693 kb on - strand, within BPHYT_RS29755 at 2229.693 kb on - strand, within BPHYT_RS29755 at 2229.788 kb on + strand, within BPHYT_RS29755
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,226,460 + BPHYT_RS29745 0.38 -3.1 2,226,673 + BPHYT_RS29745 0.56 -3.0 2,227,061 - BPHYT_RS29745 0.89 -5.3 2,227,087 + -1.1 2,227,087 + -2.0 2,227,339 - -5.1 2,227,564 + BPHYT_RS29750 0.22 -1.7 2,227,565 - BPHYT_RS29750 0.22 -3.5 2,227,867 + BPHYT_RS29750 0.39 -3.8 2,227,867 + BPHYT_RS29750 0.39 -2.7 2,228,250 + BPHYT_RS29750 0.61 -5.6 2,228,749 - BPHYT_RS29750 0.90 -2.1 2,228,749 - BPHYT_RS29750 0.90 -3.3 2,228,929 + -1.6 2,228,929 + -4.4 2,228,933 + -3.2 2,228,934 - -2.4 2,228,934 - -0.5 2,228,965 + -2.7 2,228,966 - +0.0 2,229,185 - +0.0 2,229,451 + -0.3 2,229,693 - BPHYT_RS29755 0.11 +1.0 2,229,693 - BPHYT_RS29755 0.11 -1.4 2,229,788 + BPHYT_RS29755 0.17 -1.5
Or see this region's nucleotide sequence