Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23830 and BPHYT_RS23835 are separated by 73 nucleotides BPHYT_RS23835 and BPHYT_RS23840 are separated by 161 nucleotides BPHYT_RS23840 and BPHYT_RS23845 are separated by 672 nucleotides
BPHYT_RS23830: BPHYT_RS23830 - acetyl-CoA hydrolase, at 913,909 to 915,423
_RS23830
BPHYT_RS23835: BPHYT_RS23835 - membrane protein, at 915,497 to 915,751
_RS23835
BPHYT_RS23840: BPHYT_RS23840 - methyltransferase, at 915,913 to 916,620
_RS23840
BPHYT_RS23845: BPHYT_RS23845 - hypothetical protein, at 917,293 to 917,817
_RS23845
Position (kb) 915
916
917 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 915.001 kb on + strand, within BPHYT_RS23830 at 915.369 kb on + strand at 915.477 kb on + strand at 915.799 kb on + strand at 915.851 kb on + strand at 915.852 kb on - strand at 916.042 kb on - strand at 916.081 kb on - strand, within BPHYT_RS23840 at 916.081 kb on - strand at 916.112 kb on + strand, within BPHYT_RS23840 at 916.112 kb on + strand, within BPHYT_RS23840 at 916.113 kb on - strand, within BPHYT_RS23840 at 916.181 kb on + strand, within BPHYT_RS23840 at 916.427 kb on + strand, within BPHYT_RS23840 at 916.663 kb on + strand at 916.664 kb on - strand at 916.945 kb on - strand at 916.945 kb on - strand at 916.945 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 915,001 + BPHYT_RS23830 0.72 -0.4 915,369 + -0.4 915,477 + -0.3 915,799 + +0.5 915,851 + +0.0 915,852 - -0.5 916,042 - -0.8 916,081 - BPHYT_RS23840 0.24 -3.7 916,081 - +0.5 916,112 + BPHYT_RS23840 0.28 -0.3 916,112 + BPHYT_RS23840 0.28 -2.0 916,113 - BPHYT_RS23840 0.28 -1.8 916,181 + BPHYT_RS23840 0.38 -3.7 916,427 + BPHYT_RS23840 0.73 -3.6 916,663 + +0.9 916,664 - -0.3 916,945 - -0.2 916,945 - -0.1 916,945 - +0.8
Or see this region's nucleotide sequence