Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18880 and BPHYT_RS18885 are separated by 43 nucleotides BPHYT_RS18885 and BPHYT_RS18890 are separated by 10 nucleotides BPHYT_RS18890 and BPHYT_RS18895 are separated by 92 nucleotides
BPHYT_RS18880: BPHYT_RS18880 - flagellar hook protein FlgE, at 4,242,509 to 4,244,020
_RS18880
BPHYT_RS18885: BPHYT_RS18885 - flagellar basal body rod modification protein FlgD, at 4,244,064 to 4,244,786
_RS18885
BPHYT_RS18890: BPHYT_RS18890 - flagellar basal body rod protein FlgC, at 4,244,797 to 4,245,222
_RS18890
BPHYT_RS18895: BPHYT_RS18895 - flagellar basal body rod protein FlgB, at 4,245,315 to 4,245,809
_RS18895
Position (kb) 4244
4245 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4243.140 kb on + strand, within BPHYT_RS18880 at 4243.140 kb on + strand, within BPHYT_RS18880 at 4243.140 kb on + strand, within BPHYT_RS18880 at 4243.176 kb on + strand, within BPHYT_RS18880 at 4243.176 kb on + strand, within BPHYT_RS18880 at 4243.176 kb on + strand, within BPHYT_RS18880 at 4243.197 kb on + strand, within BPHYT_RS18880 at 4243.198 kb on - strand, within BPHYT_RS18880 at 4243.203 kb on + strand, within BPHYT_RS18880 at 4243.203 kb on + strand, within BPHYT_RS18880 at 4243.203 kb on + strand, within BPHYT_RS18880 at 4243.204 kb on - strand, within BPHYT_RS18880 at 4243.333 kb on - strand, within BPHYT_RS18880 at 4243.410 kb on + strand, within BPHYT_RS18880 at 4243.410 kb on + strand, within BPHYT_RS18880 at 4243.411 kb on - strand, within BPHYT_RS18880 at 4243.411 kb on - strand, within BPHYT_RS18880 at 4243.723 kb on - strand, within BPHYT_RS18880 at 4243.739 kb on - strand, within BPHYT_RS18880 at 4243.869 kb on - strand at 4243.893 kb on + strand at 4243.894 kb on - strand at 4243.894 kb on - strand at 4243.936 kb on + strand at 4244.032 kb on + strand at 4244.033 kb on - strand at 4244.130 kb on - strand at 4244.227 kb on + strand, within BPHYT_RS18885 at 4244.228 kb on - strand, within BPHYT_RS18885 at 4244.228 kb on - strand, within BPHYT_RS18885 at 4244.547 kb on - strand, within BPHYT_RS18885 at 4244.547 kb on - strand, within BPHYT_RS18885 at 4244.726 kb on + strand at 4244.727 kb on - strand at 4244.933 kb on + strand, within BPHYT_RS18890 at 4244.933 kb on + strand, within BPHYT_RS18890 at 4244.934 kb on - strand, within BPHYT_RS18890 at 4245.095 kb on + strand, within BPHYT_RS18890 at 4245.096 kb on - strand, within BPHYT_RS18890 at 4245.289 kb on - strand at 4245.316 kb on + strand at 4245.317 kb on - strand at 4245.317 kb on - strand at 4245.437 kb on + strand, within BPHYT_RS18895 at 4245.437 kb on + strand, within BPHYT_RS18895 at 4245.698 kb on - strand, within BPHYT_RS18895 at 4245.698 kb on - strand, within BPHYT_RS18895
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 4,243,140 + BPHYT_RS18880 0.42 -4.4 4,243,140 + BPHYT_RS18880 0.42 -1.8 4,243,140 + BPHYT_RS18880 0.42 -3.1 4,243,176 + BPHYT_RS18880 0.44 -3.1 4,243,176 + BPHYT_RS18880 0.44 -1.8 4,243,176 + BPHYT_RS18880 0.44 -3.0 4,243,197 + BPHYT_RS18880 0.46 -1.0 4,243,198 - BPHYT_RS18880 0.46 -2.1 4,243,203 + BPHYT_RS18880 0.46 -0.1 4,243,203 + BPHYT_RS18880 0.46 -4.2 4,243,203 + BPHYT_RS18880 0.46 -0.9 4,243,204 - BPHYT_RS18880 0.46 -2.4 4,243,333 - BPHYT_RS18880 0.54 -2.4 4,243,410 + BPHYT_RS18880 0.60 -1.9 4,243,410 + BPHYT_RS18880 0.60 -2.0 4,243,411 - BPHYT_RS18880 0.60 -2.2 4,243,411 - BPHYT_RS18880 0.60 -2.8 4,243,723 - BPHYT_RS18880 0.80 -2.8 4,243,739 - BPHYT_RS18880 0.81 -2.7 4,243,869 - -2.5 4,243,893 + -2.4 4,243,894 - -3.2 4,243,894 - -4.0 4,243,936 + -1.5 4,244,032 + -2.5 4,244,033 - -1.6 4,244,130 - -1.3 4,244,227 + BPHYT_RS18885 0.23 -3.5 4,244,228 - BPHYT_RS18885 0.23 -2.8 4,244,228 - BPHYT_RS18885 0.23 -4.2 4,244,547 - BPHYT_RS18885 0.67 -2.7 4,244,547 - BPHYT_RS18885 0.67 -3.9 4,244,726 + -2.4 4,244,727 - -4.2 4,244,933 + BPHYT_RS18890 0.32 -2.4 4,244,933 + BPHYT_RS18890 0.32 -2.9 4,244,934 - BPHYT_RS18890 0.32 -3.5 4,245,095 + BPHYT_RS18890 0.70 -3.2 4,245,096 - BPHYT_RS18890 0.70 -2.3 4,245,289 - -0.3 4,245,316 + -3.1 4,245,317 - -1.0 4,245,317 - -1.2 4,245,437 + BPHYT_RS18895 0.25 -1.4 4,245,437 + BPHYT_RS18895 0.25 -1.0 4,245,698 - BPHYT_RS18895 0.77 -4.1 4,245,698 - BPHYT_RS18895 0.77 -3.5
Or see this region's nucleotide sequence