Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18790 and BPHYT_RS18795 are separated by 2 nucleotides BPHYT_RS18795 and BPHYT_RS18800 are separated by 70 nucleotides BPHYT_RS18800 and BPHYT_RS18805 are separated by 485 nucleotides
BPHYT_RS18790: BPHYT_RS18790 - ATP synthase, at 4,224,556 to 4,226,205
_RS18790
BPHYT_RS18795: BPHYT_RS18795 - flagellar export protein FliJ, at 4,226,208 to 4,226,657
_RS18795
BPHYT_RS18800: BPHYT_RS18800 - flagellar hook-length control protein, at 4,226,728 to 4,228,221
_RS18800
BPHYT_RS18805: BPHYT_RS18805 - flagellar basal body protein FliL, at 4,228,707 to 4,229,207
_RS18805
Position (kb) 4226
4227
4228
4229 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4225.807 kb on + strand, within BPHYT_RS18790 at 4225.808 kb on - strand, within BPHYT_RS18790 at 4225.808 kb on - strand, within BPHYT_RS18790 at 4225.808 kb on - strand, within BPHYT_RS18790 at 4225.809 kb on + strand, within BPHYT_RS18790 at 4225.809 kb on + strand, within BPHYT_RS18790 at 4225.810 kb on - strand, within BPHYT_RS18790 at 4225.810 kb on - strand, within BPHYT_RS18790 at 4225.918 kb on + strand, within BPHYT_RS18790 at 4225.998 kb on + strand, within BPHYT_RS18790 at 4225.998 kb on + strand, within BPHYT_RS18790 at 4225.999 kb on - strand, within BPHYT_RS18790 at 4225.999 kb on - strand, within BPHYT_RS18790 at 4226.352 kb on + strand, within BPHYT_RS18795 at 4226.353 kb on - strand, within BPHYT_RS18795 at 4226.539 kb on - strand, within BPHYT_RS18795 at 4226.727 kb on + strand at 4226.728 kb on - strand at 4227.105 kb on + strand, within BPHYT_RS18800 at 4227.106 kb on - strand, within BPHYT_RS18800 at 4227.362 kb on + strand, within BPHYT_RS18800 at 4227.362 kb on + strand, within BPHYT_RS18800 at 4227.362 kb on + strand, within BPHYT_RS18800 at 4227.366 kb on + strand, within BPHYT_RS18800 at 4227.367 kb on - strand, within BPHYT_RS18800 at 4227.448 kb on - strand, within BPHYT_RS18800 at 4227.774 kb on + strand, within BPHYT_RS18800 at 4227.775 kb on - strand, within BPHYT_RS18800 at 4227.775 kb on - strand, within BPHYT_RS18800 at 4227.899 kb on + strand, within BPHYT_RS18800 at 4227.900 kb on - strand, within BPHYT_RS18800 at 4228.178 kb on + strand at 4228.179 kb on - strand at 4228.294 kb on + strand at 4228.499 kb on - strand at 4228.499 kb on - strand at 4228.524 kb on + strand at 4228.555 kb on + strand at 4228.555 kb on + strand at 4228.612 kb on + strand at 4228.684 kb on + strand at 4228.793 kb on + strand, within BPHYT_RS18805 at 4228.979 kb on - strand, within BPHYT_RS18805 at 4228.979 kb on - strand, within BPHYT_RS18805 at 4229.032 kb on + strand, within BPHYT_RS18805 at 4229.032 kb on + strand, within BPHYT_RS18805 at 4229.033 kb on - strand, within BPHYT_RS18805
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 4,225,807 + BPHYT_RS18790 0.76 -3.1 4,225,808 - BPHYT_RS18790 0.76 -3.0 4,225,808 - BPHYT_RS18790 0.76 +0.2 4,225,808 - BPHYT_RS18790 0.76 -2.3 4,225,809 + BPHYT_RS18790 0.76 -2.6 4,225,809 + BPHYT_RS18790 0.76 -3.0 4,225,810 - BPHYT_RS18790 0.76 -2.9 4,225,810 - BPHYT_RS18790 0.76 -0.7 4,225,918 + BPHYT_RS18790 0.83 -3.2 4,225,998 + BPHYT_RS18790 0.87 -3.9 4,225,998 + BPHYT_RS18790 0.87 -2.8 4,225,999 - BPHYT_RS18790 0.87 -2.1 4,225,999 - BPHYT_RS18790 0.87 -2.5 4,226,352 + BPHYT_RS18795 0.32 -4.0 4,226,353 - BPHYT_RS18795 0.32 -2.5 4,226,539 - BPHYT_RS18795 0.74 -2.9 4,226,727 + +0.9 4,226,728 - -3.7 4,227,105 + BPHYT_RS18800 0.25 -4.2 4,227,106 - BPHYT_RS18800 0.25 -1.3 4,227,362 + BPHYT_RS18800 0.42 -3.4 4,227,362 + BPHYT_RS18800 0.42 -3.4 4,227,362 + BPHYT_RS18800 0.42 -2.7 4,227,366 + BPHYT_RS18800 0.43 -5.1 4,227,367 - BPHYT_RS18800 0.43 -1.4 4,227,448 - BPHYT_RS18800 0.48 -3.2 4,227,774 + BPHYT_RS18800 0.70 -2.3 4,227,775 - BPHYT_RS18800 0.70 -3.1 4,227,775 - BPHYT_RS18800 0.70 -2.4 4,227,899 + BPHYT_RS18800 0.78 -1.9 4,227,900 - BPHYT_RS18800 0.78 -2.9 4,228,178 + -1.9 4,228,179 - -1.8 4,228,294 + -0.0 4,228,499 - +0.1 4,228,499 - -0.8 4,228,524 + +0.0 4,228,555 + -0.3 4,228,555 + -0.3 4,228,612 + +0.0 4,228,684 + +0.4 4,228,793 + BPHYT_RS18805 0.17 +1.0 4,228,979 - BPHYT_RS18805 0.54 -3.0 4,228,979 - BPHYT_RS18805 0.54 -1.7 4,229,032 + BPHYT_RS18805 0.65 +0.7 4,229,032 + BPHYT_RS18805 0.65 +0.1 4,229,033 - BPHYT_RS18805 0.65 -2.1
Or see this region's nucleotide sequence