Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18745 and BPHYT_RS18750 are separated by 3 nucleotides BPHYT_RS18750 and BPHYT_RS18755 overlap by 1 nucleotides BPHYT_RS18755 and BPHYT_RS18760 are separated by 91 nucleotides BPHYT_RS18760 and BPHYT_RS18765 overlap by 4 nucleotides
BPHYT_RS18745: BPHYT_RS18745 - coniferyl aldehyde dehydrogenase, at 4,216,245 to 4,217,663
_RS18745
BPHYT_RS18750: BPHYT_RS18750 - flagellar biosynthetic protein FlhB, at 4,217,667 to 4,218,026
_RS18750
BPHYT_RS18755: BPHYT_RS18755 - flagellar hook-length control protein FliK, at 4,218,026 to 4,219,459
_RS18755
BPHYT_RS18760: BPHYT_RS18760 - flagellar biosynthesis protein FliT, at 4,219,551 to 4,219,877
_RS18760
BPHYT_RS18765: BPHYT_RS18765 - flagellar biosynthesis protein FliS, at 4,219,874 to 4,220,308
_RS18765
Position (kb) 4218
4219
4220 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4217.083 kb on - strand, within BPHYT_RS18745 at 4217.319 kb on + strand, within BPHYT_RS18745 at 4217.319 kb on + strand, within BPHYT_RS18745 at 4217.319 kb on + strand, within BPHYT_RS18745 at 4217.320 kb on - strand, within BPHYT_RS18745 at 4217.379 kb on + strand, within BPHYT_RS18745 at 4217.379 kb on + strand, within BPHYT_RS18745 at 4217.380 kb on - strand, within BPHYT_RS18745 at 4217.385 kb on + strand, within BPHYT_RS18745 at 4217.523 kb on + strand at 4217.524 kb on - strand at 4217.813 kb on - strand, within BPHYT_RS18750 at 4217.813 kb on - strand, within BPHYT_RS18750 at 4217.887 kb on + strand, within BPHYT_RS18750 at 4217.887 kb on + strand, within BPHYT_RS18750 at 4217.887 kb on + strand, within BPHYT_RS18750 at 4217.939 kb on - strand, within BPHYT_RS18750 at 4217.987 kb on - strand, within BPHYT_RS18750 at 4217.987 kb on - strand, within BPHYT_RS18750 at 4218.157 kb on - strand at 4219.397 kb on - strand at 4219.498 kb on + strand at 4219.498 kb on + strand at 4219.499 kb on - strand at 4219.499 kb on - strand at 4219.520 kb on + strand at 4219.520 kb on + strand at 4219.520 kb on + strand at 4219.685 kb on - strand, within BPHYT_RS18760 at 4219.685 kb on - strand, within BPHYT_RS18760 at 4219.759 kb on + strand, within BPHYT_RS18760 at 4220.030 kb on - strand, within BPHYT_RS18765 at 4220.030 kb on - strand, within BPHYT_RS18765 at 4220.030 kb on - strand, within BPHYT_RS18765 at 4220.030 kb on - strand, within BPHYT_RS18765 at 4220.030 kb on - strand, within BPHYT_RS18765 at 4220.032 kb on - strand, within BPHYT_RS18765 at 4220.032 kb on - strand, within BPHYT_RS18765 at 4220.032 kb on - strand, within BPHYT_RS18765 at 4220.036 kb on - strand, within BPHYT_RS18765 at 4220.036 kb on - strand, within BPHYT_RS18765 at 4220.038 kb on - strand, within BPHYT_RS18765 at 4220.038 kb on - strand, within BPHYT_RS18765 at 4220.275 kb on - strand at 4220.275 kb on - strand at 4220.388 kb on - strand at 4220.388 kb on - strand at 4220.388 kb on - strand at 4220.388 kb on - strand at 4220.420 kb on + strand at 4220.421 kb on - strand at 4220.440 kb on + strand at 4220.441 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 4,217,083 - BPHYT_RS18745 0.59 -0.0 4,217,319 + BPHYT_RS18745 0.76 +0.5 4,217,319 + BPHYT_RS18745 0.76 +0.2 4,217,319 + BPHYT_RS18745 0.76 +0.0 4,217,320 - BPHYT_RS18745 0.76 +0.2 4,217,379 + BPHYT_RS18745 0.80 +1.8 4,217,379 + BPHYT_RS18745 0.80 +0.0 4,217,380 - BPHYT_RS18745 0.80 -0.1 4,217,385 + BPHYT_RS18745 0.80 +0.1 4,217,523 + -0.8 4,217,524 - +0.2 4,217,813 - BPHYT_RS18750 0.41 -0.4 4,217,813 - BPHYT_RS18750 0.41 -0.8 4,217,887 + BPHYT_RS18750 0.61 +0.3 4,217,887 + BPHYT_RS18750 0.61 -0.3 4,217,887 + BPHYT_RS18750 0.61 -0.5 4,217,939 - BPHYT_RS18750 0.76 +0.1 4,217,987 - BPHYT_RS18750 0.89 +0.1 4,217,987 - BPHYT_RS18750 0.89 -0.1 4,218,157 - -1.6 4,219,397 - +0.6 4,219,498 + -1.3 4,219,498 + -0.9 4,219,499 - +0.6 4,219,499 - -0.8 4,219,520 + -0.9 4,219,520 + -0.7 4,219,520 + -0.1 4,219,685 - BPHYT_RS18760 0.41 -2.2 4,219,685 - BPHYT_RS18760 0.41 -4.5 4,219,759 + BPHYT_RS18760 0.64 -3.2 4,220,030 - BPHYT_RS18765 0.36 -1.1 4,220,030 - BPHYT_RS18765 0.36 -0.9 4,220,030 - BPHYT_RS18765 0.36 -0.9 4,220,030 - BPHYT_RS18765 0.36 -0.6 4,220,030 - BPHYT_RS18765 0.36 -1.6 4,220,032 - BPHYT_RS18765 0.36 -0.6 4,220,032 - BPHYT_RS18765 0.36 -1.0 4,220,032 - BPHYT_RS18765 0.36 -0.1 4,220,036 - BPHYT_RS18765 0.37 -1.2 4,220,036 - BPHYT_RS18765 0.37 -1.4 4,220,038 - BPHYT_RS18765 0.38 -1.0 4,220,038 - BPHYT_RS18765 0.38 -0.8 4,220,275 - -1.4 4,220,275 - -2.2 4,220,388 - +2.2 4,220,388 - +0.8 4,220,388 - +2.5 4,220,388 - +1.4 4,220,420 + -1.4 4,220,421 - +2.0 4,220,440 + -1.2 4,220,441 - +1.5
Or see this region's nucleotide sequence