Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18585 and BPHYT_RS18590 are separated by 22 nucleotides BPHYT_RS18590 and BPHYT_RS18595 are separated by 749 nucleotides BPHYT_RS18595 and BPHYT_RS18600 overlap by 4 nucleotides BPHYT_RS18600 and BPHYT_RS18605 are separated by 26 nucleotides
BPHYT_RS18585: BPHYT_RS18585 - hypothetical protein, at 4,180,631 to 4,181,821
_RS18585
BPHYT_RS18590: BPHYT_RS18590 - hypothetical protein, at 4,181,844 to 4,182,038
_RS18590
BPHYT_RS18595: BPHYT_RS18595 - multifunctional CCA protein, at 4,182,788 to 4,184,020
_RS18595
BPHYT_RS18600: BPHYT_RS18600 - glutathione S-transferase, at 4,184,017 to 4,184,670
_RS18600
BPHYT_RS18605: BPHYT_RS18605 - NAD-dependent dehydratase, at 4,184,697 to 4,185,650
_RS18605
Position (kb)
4182
4183
4184
4185 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4181.813 kb on - strand at 4181.813 kb on - strand at 4181.813 kb on - strand at 4181.813 kb on - strand at 4181.865 kb on - strand, within BPHYT_RS18590 at 4182.036 kb on + strand at 4182.036 kb on + strand at 4182.127 kb on - strand at 4182.158 kb on - strand at 4182.158 kb on - strand at 4182.714 kb on + strand at 4184.011 kb on - strand at 4184.115 kb on - strand, within BPHYT_RS18600 at 4184.611 kb on + strand at 4184.643 kb on - strand at 4184.677 kb on + strand at 4184.678 kb on - strand at 4184.758 kb on + strand at 4184.971 kb on + strand, within BPHYT_RS18605 at 4185.019 kb on + strand, within BPHYT_RS18605 at 4185.020 kb on - strand, within BPHYT_RS18605 at 4185.020 kb on - strand, within BPHYT_RS18605
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 4,181,813 - -1.5 4,181,813 - -1.1 4,181,813 - -0.4 4,181,813 - -0.6 4,181,865 - BPHYT_RS18590 0.11 -2.2 4,182,036 + -0.5 4,182,036 + -0.8 4,182,127 - -0.1 4,182,158 - +1.1 4,182,158 - +0.1 4,182,714 + -0.7 4,184,011 - +0.4 4,184,115 - BPHYT_RS18600 0.15 -0.6 4,184,611 + -3.0 4,184,643 - -0.4 4,184,677 + -1.7 4,184,678 - +0.1 4,184,758 + +0.5 4,184,971 + BPHYT_RS18605 0.29 -0.3 4,185,019 + BPHYT_RS18605 0.34 -0.3 4,185,020 - BPHYT_RS18605 0.34 +0.1 4,185,020 - BPHYT_RS18605 0.34 -0.0
Or see this region's nucleotide sequence