Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16835 and BPHYT_RS16840 are separated by 95 nucleotides BPHYT_RS16840 and BPHYT_RS16845 are separated by 278 nucleotides
BPHYT_RS16835: BPHYT_RS16835 - glyceraldehyde-3-phosphate dehydrogenase, at 3,819,755 to 3,820,765
_RS16835
BPHYT_RS16840: BPHYT_RS16840 - transketolase, at 3,820,861 to 3,822,882
_RS16840
BPHYT_RS16845: BPHYT_RS16845 - spermidine synthase, at 3,823,161 to 3,824,033
_RS16845
Position (kb)
3820
3821
3822
3823 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3819.869 kb on - strand, within BPHYT_RS16835 at 3820.535 kb on + strand, within BPHYT_RS16835 at 3820.566 kb on + strand, within BPHYT_RS16835 at 3820.567 kb on - strand, within BPHYT_RS16835 at 3820.567 kb on - strand, within BPHYT_RS16835 at 3820.618 kb on - strand, within BPHYT_RS16835 at 3820.618 kb on - strand, within BPHYT_RS16835 at 3820.702 kb on - strand at 3820.770 kb on + strand at 3820.771 kb on - strand at 3820.771 kb on - strand at 3820.792 kb on + strand at 3820.889 kb on - strand at 3820.889 kb on - strand at 3821.241 kb on - strand, within BPHYT_RS16840 at 3821.649 kb on - strand, within BPHYT_RS16840 at 3821.930 kb on + strand, within BPHYT_RS16840 at 3822.246 kb on - strand, within BPHYT_RS16840 at 3822.246 kb on - strand, within BPHYT_RS16840 at 3822.419 kb on + strand, within BPHYT_RS16840 at 3822.420 kb on - strand, within BPHYT_RS16840 at 3823.024 kb on + strand at 3823.025 kb on - strand at 3823.111 kb on + strand at 3823.111 kb on + strand at 3823.112 kb on - strand at 3823.132 kb on + strand at 3823.203 kb on + strand at 3823.495 kb on + strand, within BPHYT_RS16845 at 3823.496 kb on - strand, within BPHYT_RS16845 at 3823.496 kb on - strand, within BPHYT_RS16845 at 3823.512 kb on + strand, within BPHYT_RS16845 at 3823.584 kb on + strand, within BPHYT_RS16845 at 3823.585 kb on - strand, within BPHYT_RS16845 at 3823.665 kb on + strand, within BPHYT_RS16845 at 3823.665 kb on + strand, within BPHYT_RS16845 at 3823.666 kb on - strand, within BPHYT_RS16845 at 3823.666 kb on - strand, within BPHYT_RS16845 at 3823.680 kb on + strand, within BPHYT_RS16845
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 3,819,869 - BPHYT_RS16835 0.11 -2.5 3,820,535 + BPHYT_RS16835 0.77 +1.2 3,820,566 + BPHYT_RS16835 0.80 -2.0 3,820,567 - BPHYT_RS16835 0.80 -1.9 3,820,567 - BPHYT_RS16835 0.80 -1.8 3,820,618 - BPHYT_RS16835 0.85 -1.2 3,820,618 - BPHYT_RS16835 0.85 -1.7 3,820,702 - -1.4 3,820,770 + -1.7 3,820,771 - -1.0 3,820,771 - -1.9 3,820,792 + -2.7 3,820,889 - -0.3 3,820,889 - -0.6 3,821,241 - BPHYT_RS16840 0.19 -3.7 3,821,649 - BPHYT_RS16840 0.39 -1.9 3,821,930 + BPHYT_RS16840 0.53 -2.5 3,822,246 - BPHYT_RS16840 0.68 -2.9 3,822,246 - BPHYT_RS16840 0.68 -2.4 3,822,419 + BPHYT_RS16840 0.77 -1.8 3,822,420 - BPHYT_RS16840 0.77 -1.4 3,823,024 + -1.7 3,823,025 - -0.9 3,823,111 + -0.1 3,823,111 + +0.2 3,823,112 - -0.9 3,823,132 + +0.6 3,823,203 + +0.3 3,823,495 + BPHYT_RS16845 0.38 -0.1 3,823,496 - BPHYT_RS16845 0.38 -1.3 3,823,496 - BPHYT_RS16845 0.38 -1.1 3,823,512 + BPHYT_RS16845 0.40 -0.6 3,823,584 + BPHYT_RS16845 0.48 +1.0 3,823,585 - BPHYT_RS16845 0.49 +0.6 3,823,665 + BPHYT_RS16845 0.58 +0.0 3,823,665 + BPHYT_RS16845 0.58 +0.6 3,823,666 - BPHYT_RS16845 0.58 -0.4 3,823,666 - BPHYT_RS16845 0.58 +0.5 3,823,680 + BPHYT_RS16845 0.59 -0.2
Or see this region's nucleotide sequence