Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16120 and BPHYT_RS16125 are separated by 332 nucleotides BPHYT_RS16125 and BPHYT_RS16130 are separated by 59 nucleotides BPHYT_RS16130 and BPHYT_RS16135 overlap by 4 nucleotides BPHYT_RS16135 and BPHYT_RS16140 overlap by 8 nucleotides
BPHYT_RS16120: BPHYT_RS16120 - sorbitol dehydrogenase, D-fructose forming (EC 1.1.1.14) (from data) , at 3,657,289 to 3,658,071
_RS16120
BPHYT_RS16125: BPHYT_RS16125 - Fur family transcriptional regulator, at 3,658,404 to 3,658,898
_RS16125
BPHYT_RS16130: BPHYT_RS16130 - cation ABC transporter substrate-binding protein, at 3,658,958 to 3,659,926
_RS16130
BPHYT_RS16135: BPHYT_RS16135 - ABC transporter ATP-binding protein, at 3,659,923 to 3,660,864
_RS16135
BPHYT_RS16140: BPHYT_RS16140 - ABC transporter permease, at 3,660,857 to 3,661,639
_RS16140
Position (kb)
3658
3659
3660 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3657.993 kb on - strand at 3658.253 kb on + strand at 3658.253 kb on + strand at 3658.254 kb on - strand at 3658.254 kb on - strand at 3658.254 kb on - strand at 3658.254 kb on - strand at 3658.257 kb on - strand at 3658.322 kb on - strand at 3658.507 kb on - strand, within BPHYT_RS16125 at 3658.507 kb on - strand, within BPHYT_RS16125 at 3658.545 kb on + strand, within BPHYT_RS16125 at 3658.546 kb on - strand, within BPHYT_RS16125 at 3658.600 kb on + strand, within BPHYT_RS16125 at 3658.600 kb on + strand, within BPHYT_RS16125 at 3658.600 kb on + strand, within BPHYT_RS16125 at 3658.600 kb on + strand, within BPHYT_RS16125 at 3658.600 kb on + strand, within BPHYT_RS16125 at 3658.914 kb on + strand at 3658.914 kb on + strand at 3658.914 kb on + strand at 3658.914 kb on + strand at 3658.915 kb on - strand at 3659.243 kb on + strand, within BPHYT_RS16130 at 3659.244 kb on - strand, within BPHYT_RS16130 at 3659.369 kb on + strand, within BPHYT_RS16130 at 3659.705 kb on + strand, within BPHYT_RS16130 at 3659.705 kb on + strand, within BPHYT_RS16130 at 3659.705 kb on + strand, within BPHYT_RS16130 at 3659.705 kb on + strand, within BPHYT_RS16130 at 3660.194 kb on - strand, within BPHYT_RS16135 at 3660.195 kb on + strand, within BPHYT_RS16135 at 3660.196 kb on - strand, within BPHYT_RS16135 at 3660.377 kb on + strand, within BPHYT_RS16135 at 3660.378 kb on - strand, within BPHYT_RS16135 at 3660.593 kb on - strand, within BPHYT_RS16135 at 3660.593 kb on - strand, within BPHYT_RS16135 at 3660.593 kb on - strand, within BPHYT_RS16135 at 3660.635 kb on + strand, within BPHYT_RS16135 at 3660.636 kb on - strand, within BPHYT_RS16135
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 3,657,993 - +0.5 3,658,253 + +0.0 3,658,253 + -1.8 3,658,254 - -0.1 3,658,254 - +0.2 3,658,254 - -0.5 3,658,254 - +0.0 3,658,257 - -0.1 3,658,322 - -1.0 3,658,507 - BPHYT_RS16125 0.21 -0.9 3,658,507 - BPHYT_RS16125 0.21 -1.0 3,658,545 + BPHYT_RS16125 0.28 -0.1 3,658,546 - BPHYT_RS16125 0.29 -1.7 3,658,600 + BPHYT_RS16125 0.40 +0.4 3,658,600 + BPHYT_RS16125 0.40 -0.2 3,658,600 + BPHYT_RS16125 0.40 +0.2 3,658,600 + BPHYT_RS16125 0.40 +0.0 3,658,600 + BPHYT_RS16125 0.40 -0.1 3,658,914 + -1.2 3,658,914 + -0.8 3,658,914 + -0.4 3,658,914 + -0.5 3,658,915 - -2.5 3,659,243 + BPHYT_RS16130 0.29 -1.2 3,659,244 - BPHYT_RS16130 0.30 -1.1 3,659,369 + BPHYT_RS16130 0.42 -1.5 3,659,705 + BPHYT_RS16130 0.77 -2.9 3,659,705 + BPHYT_RS16130 0.77 -1.1 3,659,705 + BPHYT_RS16130 0.77 -1.7 3,659,705 + BPHYT_RS16130 0.77 -0.5 3,660,194 - BPHYT_RS16135 0.29 -0.7 3,660,195 + BPHYT_RS16135 0.29 -2.9 3,660,196 - BPHYT_RS16135 0.29 -1.0 3,660,377 + BPHYT_RS16135 0.48 -1.4 3,660,378 - BPHYT_RS16135 0.48 -1.2 3,660,593 - BPHYT_RS16135 0.71 -1.8 3,660,593 - BPHYT_RS16135 0.71 -1.1 3,660,593 - BPHYT_RS16135 0.71 -1.4 3,660,635 + BPHYT_RS16135 0.76 -0.9 3,660,636 - BPHYT_RS16135 0.76 -1.2
Or see this region's nucleotide sequence