Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12615 and BPHYT_RS12620 are separated by 43 nucleotides BPHYT_RS12620 and BPHYT_RS12625 are separated by 35 nucleotides BPHYT_RS12625 and BPHYT_RS12630 are separated by 6 nucleotides BPHYT_RS12630 and BPHYT_RS12635 are separated by 47 nucleotides
BPHYT_RS12615: BPHYT_RS12615 - dihydrodipicolinate synthase, at 2,879,263 to 2,880,189
_RS12615
BPHYT_RS12620: BPHYT_RS12620 - SAM-dependent methyltransferase PhcB, at 2,880,233 to 2,880,835
_RS12620
BPHYT_RS12625: BPHYT_RS12625 - tryptophanyl-tRNA synthetase, at 2,880,871 to 2,882,073
_RS12625
BPHYT_RS12630: BPHYT_RS12630 - peptidase M50, at 2,882,080 to 2,882,742
_RS12630
BPHYT_RS12635: BPHYT_RS12635 - translation factor Sua5, at 2,882,790 to 2,883,425
_RS12635
Position (kb)
2880
2881
2882
2883 Strain fitness (log2 ratio)
-1
0
1
2 at 2880.249 kb on + strand at 2880.431 kb on - strand, within BPHYT_RS12620 at 2880.830 kb on - strand at 2880.830 kb on - strand at 2880.830 kb on - strand at 2880.830 kb on - strand at 2880.901 kb on + strand at 2880.902 kb on - strand at 2880.990 kb on - strand at 2880.990 kb on - strand at 2880.990 kb on - strand at 2881.011 kb on + strand, within BPHYT_RS12625 at 2881.011 kb on + strand, within BPHYT_RS12625 at 2881.012 kb on - strand, within BPHYT_RS12625 at 2881.012 kb on - strand, within BPHYT_RS12625 at 2881.012 kb on - strand, within BPHYT_RS12625 at 2881.265 kb on + strand, within BPHYT_RS12625 at 2881.265 kb on + strand, within BPHYT_RS12625 at 2881.488 kb on - strand, within BPHYT_RS12625 at 2881.568 kb on + strand, within BPHYT_RS12625 at 2881.568 kb on + strand, within BPHYT_RS12625 at 2881.568 kb on + strand, within BPHYT_RS12625 at 2881.569 kb on - strand, within BPHYT_RS12625 at 2881.649 kb on + strand, within BPHYT_RS12625 at 2881.697 kb on + strand, within BPHYT_RS12625 at 2881.698 kb on - strand, within BPHYT_RS12625 at 2881.714 kb on - strand, within BPHYT_RS12625 at 2881.714 kb on - strand, within BPHYT_RS12625 at 2881.742 kb on + strand, within BPHYT_RS12625 at 2881.922 kb on + strand, within BPHYT_RS12625 at 2882.058 kb on + strand at 2882.058 kb on + strand at 2882.059 kb on - strand at 2882.077 kb on + strand at 2882.078 kb on - strand at 2882.756 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,880,249 + -0.3 2,880,431 - BPHYT_RS12620 0.33 +0.8 2,880,830 - +0.3 2,880,830 - +0.2 2,880,830 - -0.2 2,880,830 - +0.1 2,880,901 + -0.5 2,880,902 - +2.0 2,880,990 - -0.0 2,880,990 - +0.5 2,880,990 - +0.6 2,881,011 + BPHYT_RS12625 0.12 -0.3 2,881,011 + BPHYT_RS12625 0.12 -0.2 2,881,012 - BPHYT_RS12625 0.12 -1.6 2,881,012 - BPHYT_RS12625 0.12 -0.5 2,881,012 - BPHYT_RS12625 0.12 -0.0 2,881,265 + BPHYT_RS12625 0.33 +0.9 2,881,265 + BPHYT_RS12625 0.33 +0.6 2,881,488 - BPHYT_RS12625 0.51 +0.1 2,881,568 + BPHYT_RS12625 0.58 +0.6 2,881,568 + BPHYT_RS12625 0.58 +0.1 2,881,568 + BPHYT_RS12625 0.58 -0.8 2,881,569 - BPHYT_RS12625 0.58 -0.2 2,881,649 + BPHYT_RS12625 0.65 -0.4 2,881,697 + BPHYT_RS12625 0.69 -0.6 2,881,698 - BPHYT_RS12625 0.69 -0.1 2,881,714 - BPHYT_RS12625 0.70 +0.4 2,881,714 - BPHYT_RS12625 0.70 -0.2 2,881,742 + BPHYT_RS12625 0.72 +0.3 2,881,922 + BPHYT_RS12625 0.87 -0.7 2,882,058 + +0.2 2,882,058 + -0.2 2,882,059 - -0.7 2,882,077 + -0.3 2,882,078 - -0.1 2,882,756 - -0.6
Or see this region's nucleotide sequence