Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09095 and BPHYT_RS09100 are separated by 224 nucleotides BPHYT_RS09100 and BPHYT_RS09105 overlap by 4 nucleotides BPHYT_RS09105 and BPHYT_RS09110 are separated by 5 nucleotides BPHYT_RS09110 and BPHYT_RS09115 are separated by 11 nucleotides
BPHYT_RS09095: BPHYT_RS09095 - NADPH:quinone oxidoreductase, at 2,057,874 to 2,058,848
_RS09095
BPHYT_RS09100: BPHYT_RS09100 - stationary phase survival protein SurE, at 2,059,073 to 2,059,831
_RS09100
BPHYT_RS09105: BPHYT_RS09105 - protein-L-isoaspartate O-methyltransferase, at 2,059,828 to 2,060,880
_RS09105
BPHYT_RS09110: BPHYT_RS09110 - peptidase M23, at 2,060,886 to 2,061,833
_RS09110
BPHYT_RS09115: BPHYT_RS09115 - RNA polymerase sigma factor RpoS, at 2,061,845 to 2,062,927
_RS09115
Position (kb)
2059
2060
2061 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2058.880 kb on + strand at 2058.881 kb on - strand at 2059.010 kb on + strand at 2059.010 kb on + strand at 2059.256 kb on + strand, within BPHYT_RS09100 at 2059.256 kb on + strand, within BPHYT_RS09100 at 2059.257 kb on - strand, within BPHYT_RS09100 at 2059.315 kb on + strand, within BPHYT_RS09100 at 2059.316 kb on - strand, within BPHYT_RS09100 at 2059.316 kb on - strand, within BPHYT_RS09100 at 2060.374 kb on + strand, within BPHYT_RS09105 at 2060.375 kb on - strand, within BPHYT_RS09105 at 2060.562 kb on + strand, within BPHYT_RS09105 at 2060.563 kb on - strand, within BPHYT_RS09105 at 2060.630 kb on - strand, within BPHYT_RS09105 at 2060.630 kb on - strand, within BPHYT_RS09105 at 2060.717 kb on + strand, within BPHYT_RS09105 at 2060.717 kb on + strand, within BPHYT_RS09105 at 2060.855 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,058,880 + +1.2 2,058,881 - -0.1 2,059,010 + -0.3 2,059,010 + -0.3 2,059,256 + BPHYT_RS09100 0.24 -2.9 2,059,256 + BPHYT_RS09100 0.24 -0.1 2,059,257 - BPHYT_RS09100 0.24 -4.0 2,059,315 + BPHYT_RS09100 0.32 +0.2 2,059,316 - BPHYT_RS09100 0.32 -2.5 2,059,316 - BPHYT_RS09100 0.32 -0.8 2,060,374 + BPHYT_RS09105 0.52 +0.3 2,060,375 - BPHYT_RS09105 0.52 -5.7 2,060,562 + BPHYT_RS09105 0.70 -1.1 2,060,563 - BPHYT_RS09105 0.70 -3.3 2,060,630 - BPHYT_RS09105 0.76 -5.5 2,060,630 - BPHYT_RS09105 0.76 -2.4 2,060,717 + BPHYT_RS09105 0.84 -0.7 2,060,717 + BPHYT_RS09105 0.84 -0.8 2,060,855 + -1.7
Or see this region's nucleotide sequence