Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS06965 and BPHYT_RS06970 are separated by 266 nucleotides BPHYT_RS06970 and BPHYT_RS06975 are separated by 215 nucleotides BPHYT_RS06975 and BPHYT_RS06980 are separated by 143 nucleotides BPHYT_RS06980 and BPHYT_RS06985 are separated by 189 nucleotides
BPHYT_RS06965: BPHYT_RS06965 - cell envelope biogenesis protein TonB, at 1,575,179 to 1,575,913
_RS06965
BPHYT_RS06970: BPHYT_RS06970 - (2Fe-2S)-binding protein, at 1,576,180 to 1,576,416
_RS06970
BPHYT_RS06975: BPHYT_RS06975 - glutamate racemase, at 1,576,632 to 1,577,519
_RS06975
BPHYT_RS06980: BPHYT_RS06980 - bacterioferritin, at 1,577,663 to 1,578,142
_RS06980
BPHYT_RS06985: BPHYT_RS06985 - fumarate hydratase, at 1,578,332 to 1,579,855
_RS06985
Position (kb)
1576
1577
1578 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1576.182 kb on - strand at 1576.429 kb on - strand at 1576.429 kb on - strand at 1576.515 kb on + strand at 1576.515 kb on + strand at 1576.516 kb on - strand at 1577.075 kb on - strand, within BPHYT_RS06975 at 1577.371 kb on + strand, within BPHYT_RS06975 at 1577.372 kb on - strand, within BPHYT_RS06975 at 1577.372 kb on - strand, within BPHYT_RS06975 at 1577.520 kb on - strand at 1577.585 kb on + strand at 1577.585 kb on + strand at 1577.585 kb on + strand at 1577.585 kb on + strand at 1577.586 kb on - strand at 1577.586 kb on - strand at 1577.587 kb on + strand at 1577.618 kb on - strand at 1577.618 kb on - strand at 1577.697 kb on + strand at 1577.698 kb on - strand at 1577.698 kb on - strand at 1577.824 kb on - strand, within BPHYT_RS06980 at 1578.010 kb on - strand, within BPHYT_RS06980 at 1578.046 kb on + strand, within BPHYT_RS06980 at 1578.046 kb on + strand, within BPHYT_RS06980 at 1578.046 kb on + strand, within BPHYT_RS06980 at 1578.047 kb on - strand, within BPHYT_RS06980 at 1578.047 kb on - strand, within BPHYT_RS06980 at 1578.048 kb on + strand, within BPHYT_RS06980 at 1578.049 kb on - strand, within BPHYT_RS06980 at 1578.070 kb on - strand, within BPHYT_RS06980 at 1578.155 kb on + strand at 1578.155 kb on + strand at 1578.328 kb on + strand at 1578.456 kb on + strand at 1578.456 kb on + strand at 1578.456 kb on + strand at 1578.457 kb on - strand at 1578.457 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 1,576,182 - -0.1 1,576,429 - -0.3 1,576,429 - -1.0 1,576,515 + -0.4 1,576,515 + -0.3 1,576,516 - -0.2 1,577,075 - BPHYT_RS06975 0.50 -1.8 1,577,371 + BPHYT_RS06975 0.83 -1.5 1,577,372 - BPHYT_RS06975 0.83 -0.9 1,577,372 - BPHYT_RS06975 0.83 -2.5 1,577,520 - -0.7 1,577,585 + -0.3 1,577,585 + +1.1 1,577,585 + -0.2 1,577,585 + -2.3 1,577,586 - -0.6 1,577,586 - -0.2 1,577,587 + -0.5 1,577,618 - -0.1 1,577,618 - +2.2 1,577,697 + +0.6 1,577,698 - +0.3 1,577,698 - +2.1 1,577,824 - BPHYT_RS06980 0.34 +0.4 1,578,010 - BPHYT_RS06980 0.72 -0.4 1,578,046 + BPHYT_RS06980 0.80 +0.3 1,578,046 + BPHYT_RS06980 0.80 +1.3 1,578,046 + BPHYT_RS06980 0.80 -0.1 1,578,047 - BPHYT_RS06980 0.80 -0.3 1,578,047 - BPHYT_RS06980 0.80 +0.5 1,578,048 + BPHYT_RS06980 0.80 -0.4 1,578,049 - BPHYT_RS06980 0.80 +0.0 1,578,070 - BPHYT_RS06980 0.85 -0.3 1,578,155 + -0.2 1,578,155 + +0.6 1,578,328 + +1.0 1,578,456 + +0.7 1,578,456 + +1.7 1,578,456 + +0.4 1,578,457 - +0.4 1,578,457 - -0.6
Or see this region's nucleotide sequence