Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03275 and BPHYT_RS03280 are separated by 71 nucleotides BPHYT_RS03280 and BPHYT_RS03285 are separated by 3 nucleotides BPHYT_RS03285 and BPHYT_RS03290 are separated by 223 nucleotides
BPHYT_RS03275: BPHYT_RS03275 - metal ABC transporter permease, at 736,144 to 738,033
_RS03275
BPHYT_RS03280: BPHYT_RS03280 - mannose-1-phosphate guanylyltransferase, at 738,105 to 738,833
_RS03280
BPHYT_RS03285: BPHYT_RS03285 - aminoglycoside phosphotransferase, at 738,837 to 739,928
_RS03285
BPHYT_RS03290: BPHYT_RS03290 - LPS-assembly protein LptD, at 740,152 to 742,518
_RS03290
Position (kb) 738
739
740 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 738.107 kb on - strand at 738.107 kb on - strand at 738.191 kb on - strand, within BPHYT_RS03280 at 738.481 kb on + strand, within BPHYT_RS03280 at 738.847 kb on + strand at 738.913 kb on + strand at 738.914 kb on - strand at 738.967 kb on + strand, within BPHYT_RS03285 at 738.967 kb on + strand, within BPHYT_RS03285 at 738.968 kb on - strand, within BPHYT_RS03285 at 738.968 kb on - strand, within BPHYT_RS03285 at 739.114 kb on + strand, within BPHYT_RS03285 at 739.117 kb on + strand, within BPHYT_RS03285 at 739.190 kb on - strand, within BPHYT_RS03285 at 739.190 kb on - strand, within BPHYT_RS03285 at 739.204 kb on + strand, within BPHYT_RS03285 at 739.205 kb on - strand, within BPHYT_RS03285 at 739.968 kb on + strand at 739.968 kb on + strand at 739.969 kb on - strand at 739.969 kb on - strand at 739.970 kb on + strand at 739.970 kb on + strand at 739.970 kb on + strand at 739.970 kb on + strand at 740.055 kb on + strand at 740.055 kb on + strand at 740.059 kb on + strand at 740.149 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 738,107 - -0.1 738,107 - -0.8 738,191 - BPHYT_RS03280 0.12 -1.1 738,481 + BPHYT_RS03280 0.52 -2.4 738,847 + -1.1 738,913 + -3.2 738,914 - -1.3 738,967 + BPHYT_RS03285 0.12 -2.1 738,967 + BPHYT_RS03285 0.12 -0.9 738,968 - BPHYT_RS03285 0.12 -0.1 738,968 - BPHYT_RS03285 0.12 -1.2 739,114 + BPHYT_RS03285 0.25 -1.3 739,117 + BPHYT_RS03285 0.26 -1.4 739,190 - BPHYT_RS03285 0.32 -1.3 739,190 - BPHYT_RS03285 0.32 -2.2 739,204 + BPHYT_RS03285 0.34 -1.7 739,205 - BPHYT_RS03285 0.34 -1.0 739,968 + -5.0 739,968 + -3.9 739,969 - -4.2 739,969 - -2.7 739,970 + -1.0 739,970 + -1.4 739,970 + -3.1 739,970 + -0.9 740,055 + -2.0 740,055 + -3.0 740,059 + -1.9 740,149 + -4.6
Or see this region's nucleotide sequence