Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03170 and BPHYT_RS03175 are separated by 157 nucleotides BPHYT_RS03175 and BPHYT_RS03180 are separated by 15 nucleotides BPHYT_RS03180 and BPHYT_RS03185 are separated by 23 nucleotides BPHYT_RS03185 and BPHYT_RS03190 are separated by 182 nucleotides
BPHYT_RS03170: BPHYT_RS03170 - membrane protein, at 710,878 to 711,870
_RS03170
BPHYT_RS03175: BPHYT_RS03175 - sterol-binding protein, at 712,028 to 712,672
_RS03175
BPHYT_RS03180: BPHYT_RS03180 - hypothetical protein, at 712,688 to 714,265
_RS03180
BPHYT_RS03185: BPHYT_RS03185 - thiopurine S-methyltransferase, at 714,289 to 714,912
_RS03185
BPHYT_RS03190: BPHYT_RS03190 - FmdB family transcriptional regulator, at 715,095 to 715,472
_RS03190
Position (kb)
712
713
714
715 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 711.945 kb on + strand at 711.971 kb on + strand at 711.971 kb on + strand at 711.971 kb on + strand at 711.971 kb on + strand at 711.972 kb on - strand at 712.263 kb on + strand, within BPHYT_RS03175 at 712.263 kb on + strand, within BPHYT_RS03175 at 712.670 kb on + strand at 712.670 kb on + strand at 712.687 kb on + strand at 712.687 kb on + strand at 712.687 kb on + strand at 712.687 kb on + strand at 714.478 kb on + strand, within BPHYT_RS03185 at 714.613 kb on + strand, within BPHYT_RS03185 at 714.613 kb on + strand, within BPHYT_RS03185 at 714.627 kb on + strand, within BPHYT_RS03185 at 714.644 kb on - strand, within BPHYT_RS03185 at 714.691 kb on + strand, within BPHYT_RS03185 at 714.692 kb on - strand, within BPHYT_RS03185 at 714.750 kb on + strand, within BPHYT_RS03185 at 714.820 kb on + strand, within BPHYT_RS03185 at 714.978 kb on + strand at 715.068 kb on + strand at 715.068 kb on + strand at 715.254 kb on + strand, within BPHYT_RS03190
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 711,945 + -2.0 711,971 + -1.8 711,971 + -1.3 711,971 + -2.1 711,971 + -0.1 711,972 - -1.4 712,263 + BPHYT_RS03175 0.36 -2.2 712,263 + BPHYT_RS03175 0.36 -2.4 712,670 + +0.1 712,670 + -2.9 712,687 + -0.2 712,687 + +0.5 712,687 + +0.4 712,687 + -0.2 714,478 + BPHYT_RS03185 0.30 +0.0 714,613 + BPHYT_RS03185 0.52 +0.1 714,613 + BPHYT_RS03185 0.52 +1.0 714,627 + BPHYT_RS03185 0.54 -0.2 714,644 - BPHYT_RS03185 0.57 +0.8 714,691 + BPHYT_RS03185 0.64 -2.6 714,692 - BPHYT_RS03185 0.65 +1.3 714,750 + BPHYT_RS03185 0.74 -0.4 714,820 + BPHYT_RS03185 0.85 +0.1 714,978 + -0.2 715,068 + +1.0 715,068 + -0.0 715,254 + BPHYT_RS03190 0.42 +0.3
Or see this region's nucleotide sequence