Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01810 and BPHYT_RS01815 are separated by 46 nucleotides BPHYT_RS01815 and BPHYT_RS01820 are separated by 16 nucleotides
BPHYT_RS01810: BPHYT_RS01810 - sugar ABC transporter permease, at 410,690 to 411,649
_RS01810
BPHYT_RS01815: BPHYT_RS01815 - sugar ABC transporter permease, at 411,696 to 412,706
_RS01815
BPHYT_RS01820: BPHYT_RS01820 - sugar ABC transporter ATP-binding protein, at 412,723 to 414,357
_RS01820
Position (kb) 411
412
413 Strain fitness (log2 ratio) -1
0
1 at 410.793 kb on + strand, within BPHYT_RS01810 at 411.040 kb on - strand, within BPHYT_RS01810 at 411.319 kb on + strand, within BPHYT_RS01810 at 411.417 kb on + strand, within BPHYT_RS01810 at 411.541 kb on - strand, within BPHYT_RS01810 at 411.541 kb on - strand, within BPHYT_RS01810 at 411.565 kb on - strand at 411.602 kb on + strand at 411.793 kb on + strand at 411.841 kb on + strand, within BPHYT_RS01815 at 411.995 kb on - strand, within BPHYT_RS01815 at 412.037 kb on - strand, within BPHYT_RS01815 at 412.137 kb on + strand, within BPHYT_RS01815 at 412.137 kb on + strand, within BPHYT_RS01815 at 412.665 kb on + strand at 412.666 kb on - strand at 412.724 kb on + strand at 412.725 kb on - strand at 412.761 kb on + strand at 412.762 kb on - strand at 412.762 kb on - strand at 413.303 kb on + strand, within BPHYT_RS01820 at 413.323 kb on - strand, within BPHYT_RS01820 at 413.323 kb on - strand, within BPHYT_RS01820 at 413.705 kb on + strand, within BPHYT_RS01820
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 410,793 + BPHYT_RS01810 0.11 +0.7 411,040 - BPHYT_RS01810 0.36 -0.3 411,319 + BPHYT_RS01810 0.66 +0.9 411,417 + BPHYT_RS01810 0.76 -0.2 411,541 - BPHYT_RS01810 0.89 -0.2 411,541 - BPHYT_RS01810 0.89 -0.7 411,565 - -1.1 411,602 + -0.3 411,793 + -0.3 411,841 + BPHYT_RS01815 0.14 -0.2 411,995 - BPHYT_RS01815 0.30 -0.4 412,037 - BPHYT_RS01815 0.34 -0.4 412,137 + BPHYT_RS01815 0.44 -0.4 412,137 + BPHYT_RS01815 0.44 -1.1 412,665 + -0.2 412,666 - -0.4 412,724 + +1.5 412,725 - -1.4 412,761 + +0.2 412,762 - -0.1 412,762 - -1.3 413,303 + BPHYT_RS01820 0.35 +0.1 413,323 - BPHYT_RS01820 0.37 -0.3 413,323 - BPHYT_RS01820 0.37 -0.8 413,705 + BPHYT_RS01820 0.60 +0.0
Or see this region's nucleotide sequence