Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01575 and BPHYT_RS01580 are separated by 61 nucleotides BPHYT_RS01580 and BPHYT_RS01585 are separated by 133 nucleotides BPHYT_RS01585 and BPHYT_RS01590 are separated by 14 nucleotides BPHYT_RS01590 and BPHYT_RS01595 overlap by 4 nucleotides
BPHYT_RS01575: BPHYT_RS01575 - peptidase M48, at 358,342 to 359,142
_RS01575
BPHYT_RS01580: BPHYT_RS01580 - hypothetical protein, at 359,204 to 359,491
_RS01580
BPHYT_RS01585: BPHYT_RS01585 - major facilitator superfamily protein, at 359,625 to 361,019
_RS01585
BPHYT_RS01590: BPHYT_RS01590 - methionine biosynthesis protein MetW, at 361,034 to 361,642
_RS01590
BPHYT_RS01595: BPHYT_RS01595 - homoserine O-acetyltransferase, at 361,639 to 362,784
_RS01595
Position (kb) 359
360
361
362 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 358.856 kb on + strand, within BPHYT_RS01575 at 358.886 kb on + strand, within BPHYT_RS01575 at 358.886 kb on + strand, within BPHYT_RS01575 at 358.886 kb on + strand, within BPHYT_RS01575 at 358.886 kb on + strand, within BPHYT_RS01575 at 358.886 kb on + strand, within BPHYT_RS01575 at 358.887 kb on - strand, within BPHYT_RS01575 at 358.887 kb on - strand, within BPHYT_RS01575 at 358.887 kb on - strand, within BPHYT_RS01575 at 359.052 kb on - strand, within BPHYT_RS01575 at 359.052 kb on - strand, within BPHYT_RS01575 at 359.052 kb on - strand, within BPHYT_RS01575 at 359.159 kb on + strand at 359.160 kb on - strand at 359.160 kb on - strand at 359.160 kb on - strand at 359.304 kb on + strand, within BPHYT_RS01580 at 359.489 kb on + strand at 359.539 kb on + strand at 359.589 kb on + strand at 359.735 kb on - strand at 359.835 kb on - strand, within BPHYT_RS01585 at 359.836 kb on + strand, within BPHYT_RS01585 at 359.836 kb on + strand, within BPHYT_RS01585 at 359.837 kb on - strand, within BPHYT_RS01585 at 359.837 kb on - strand, within BPHYT_RS01585 at 359.837 kb on - strand, within BPHYT_RS01585 at 359.837 kb on - strand, within BPHYT_RS01585 at 359.996 kb on - strand, within BPHYT_RS01585 at 360.372 kb on + strand, within BPHYT_RS01585 at 360.373 kb on - strand, within BPHYT_RS01585 at 360.511 kb on + strand, within BPHYT_RS01585 at 360.711 kb on + strand, within BPHYT_RS01585 at 360.712 kb on - strand, within BPHYT_RS01585 at 360.712 kb on - strand, within BPHYT_RS01585 at 360.803 kb on - strand, within BPHYT_RS01585 at 360.987 kb on - strand at 361.023 kb on + strand at 361.024 kb on - strand at 361.024 kb on - strand at 361.066 kb on + strand at 361.067 kb on - strand at 361.067 kb on - strand at 361.067 kb on - strand at 361.067 kb on - strand at 361.185 kb on - strand, within BPHYT_RS01590 at 361.185 kb on - strand, within BPHYT_RS01590 at 361.209 kb on + strand, within BPHYT_RS01590 at 361.209 kb on + strand, within BPHYT_RS01590 at 361.210 kb on - strand, within BPHYT_RS01590 at 361.621 kb on - strand at 361.663 kb on - strand at 361.663 kb on - strand at 361.663 kb on - strand at 361.674 kb on - strand at 361.694 kb on + strand at 361.751 kb on + strand at 361.752 kb on - strand at 361.752 kb on - strand at 361.757 kb on - strand, within BPHYT_RS01595 at 361.847 kb on + strand, within BPHYT_RS01595 at 361.847 kb on + strand, within BPHYT_RS01595 at 361.940 kb on + strand, within BPHYT_RS01595 at 361.941 kb on - strand, within BPHYT_RS01595 at 361.941 kb on - strand, within BPHYT_RS01595 at 361.958 kb on + strand, within BPHYT_RS01595 at 361.982 kb on + strand, within BPHYT_RS01595 at 361.991 kb on + strand, within BPHYT_RS01595 at 361.992 kb on - strand, within BPHYT_RS01595 at 361.992 kb on - strand, within BPHYT_RS01595
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 358,856 + BPHYT_RS01575 0.64 -2.0 358,886 + BPHYT_RS01575 0.68 -0.4 358,886 + BPHYT_RS01575 0.68 -0.3 358,886 + BPHYT_RS01575 0.68 -0.2 358,886 + BPHYT_RS01575 0.68 -1.0 358,886 + BPHYT_RS01575 0.68 -1.1 358,887 - BPHYT_RS01575 0.68 -1.3 358,887 - BPHYT_RS01575 0.68 -0.0 358,887 - BPHYT_RS01575 0.68 -0.6 359,052 - BPHYT_RS01575 0.89 -0.4 359,052 - BPHYT_RS01575 0.89 -0.8 359,052 - BPHYT_RS01575 0.89 -0.8 359,159 + -1.3 359,160 - +0.1 359,160 - -0.6 359,160 - -1.2 359,304 + BPHYT_RS01580 0.35 -0.6 359,489 + +1.4 359,539 + -1.5 359,589 + +0.2 359,735 - -1.9 359,835 - BPHYT_RS01585 0.15 -1.8 359,836 + BPHYT_RS01585 0.15 -3.9 359,836 + BPHYT_RS01585 0.15 -0.4 359,837 - BPHYT_RS01585 0.15 -3.1 359,837 - BPHYT_RS01585 0.15 -2.2 359,837 - BPHYT_RS01585 0.15 -2.3 359,837 - BPHYT_RS01585 0.15 -2.6 359,996 - BPHYT_RS01585 0.27 -0.8 360,372 + BPHYT_RS01585 0.54 -6.0 360,373 - BPHYT_RS01585 0.54 -2.3 360,511 + BPHYT_RS01585 0.64 -1.7 360,711 + BPHYT_RS01585 0.78 -2.0 360,712 - BPHYT_RS01585 0.78 -2.3 360,712 - BPHYT_RS01585 0.78 -2.2 360,803 - BPHYT_RS01585 0.84 -2.1 360,987 - -1.7 361,023 + -1.5 361,024 - -0.3 361,024 - -0.8 361,066 + -0.7 361,067 - +0.8 361,067 - +0.1 361,067 - -0.3 361,067 - -0.1 361,185 - BPHYT_RS01590 0.25 -1.1 361,185 - BPHYT_RS01590 0.25 +0.1 361,209 + BPHYT_RS01590 0.29 +0.7 361,209 + BPHYT_RS01590 0.29 -1.0 361,210 - BPHYT_RS01590 0.29 -0.1 361,621 - -0.0 361,663 - +0.5 361,663 - -0.9 361,663 - -0.1 361,674 - +0.1 361,694 + +0.5 361,751 + -0.7 361,752 - -0.1 361,752 - +0.6 361,757 - BPHYT_RS01595 0.10 +1.7 361,847 + BPHYT_RS01595 0.18 +0.4 361,847 + BPHYT_RS01595 0.18 -0.0 361,940 + BPHYT_RS01595 0.26 -0.3 361,941 - BPHYT_RS01595 0.26 -0.6 361,941 - BPHYT_RS01595 0.26 -0.9 361,958 + BPHYT_RS01595 0.28 -0.3 361,982 + BPHYT_RS01595 0.30 -0.4 361,991 + BPHYT_RS01595 0.31 +0.4 361,992 - BPHYT_RS01595 0.31 +0.8 361,992 - BPHYT_RS01595 0.31 +0.4
Or see this region's nucleotide sequence