Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11460 and RR42_RS11465 overlap by 4 nucleotides RR42_RS11465 and RR42_RS11470 overlap by 4 nucleotides RR42_RS11470 and RR42_RS11475 overlap by 29 nucleotides RR42_RS11475 and RR42_RS11480 are separated by 220 nucleotides RR42_RS11480 and RR42_RS11485 are separated by 128 nucleotides
RR42_RS11460: RR42_RS11460 - pyrroloquinoline quinone biosynthesis protein PqqB, at 2,452,592 to 2,453,509
_RS11460
RR42_RS11465: RR42_RS11465 - pyrroloquinoline quinone biosynthesis protein PqqC, at 2,453,506 to 2,454,198
_RS11465
RR42_RS11470: RR42_RS11470 - pyrroloquinoline quinone biosynthesis protein PqqD, at 2,454,195 to 2,454,467
_RS11470
RR42_RS11475: RR42_RS11475 - pyrroloquinoline quinone biosynthesis protein PqqE, at 2,454,439 to 2,455,596
_RS11475
RR42_RS11480: RR42_RS11480 - cytochrome C, at 2,455,817 to 2,456,134
_RS11480
RR42_RS11485: RR42_RS11485 - dehydrogenase, at 2,456,263 to 2,458,005
_RS11485
Position (kb)
2454
2455
2456 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2453.659 kb on + strand, within RR42_RS11465 at 2453.662 kb on + strand, within RR42_RS11465 at 2453.663 kb on - strand, within RR42_RS11465 at 2453.663 kb on - strand, within RR42_RS11465 at 2453.663 kb on - strand, within RR42_RS11465 at 2453.663 kb on - strand, within RR42_RS11465 at 2453.887 kb on + strand, within RR42_RS11465 at 2453.888 kb on - strand, within RR42_RS11465 at 2453.888 kb on - strand, within RR42_RS11465 at 2453.999 kb on - strand, within RR42_RS11465 at 2454.422 kb on - strand, within RR42_RS11470 at 2454.503 kb on + strand at 2454.571 kb on + strand, within RR42_RS11475 at 2454.731 kb on - strand, within RR42_RS11475 at 2454.808 kb on - strand, within RR42_RS11475 at 2454.815 kb on + strand, within RR42_RS11475 at 2455.006 kb on + strand, within RR42_RS11475 at 2455.007 kb on - strand, within RR42_RS11475 at 2455.031 kb on - strand, within RR42_RS11475 at 2455.034 kb on - strand, within RR42_RS11475 at 2455.316 kb on + strand, within RR42_RS11475 at 2455.317 kb on - strand, within RR42_RS11475 at 2455.511 kb on + strand at 2455.784 kb on + strand at 2455.968 kb on + strand, within RR42_RS11480 at 2455.969 kb on - strand, within RR42_RS11480 at 2456.003 kb on + strand, within RR42_RS11480 at 2456.155 kb on + strand at 2456.156 kb on - strand at 2456.156 kb on - strand at 2456.159 kb on + strand at 2456.160 kb on - strand at 2456.238 kb on + strand at 2456.238 kb on + strand at 2456.238 kb on + strand at 2456.238 kb on + strand at 2456.239 kb on - strand at 2456.239 kb on - strand at 2456.243 kb on + strand at 2456.243 kb on + strand at 2456.243 kb on + strand at 2456.244 kb on - strand at 2456.384 kb on + strand at 2456.385 kb on - strand at 2456.385 kb on - strand at 2456.489 kb on + strand, within RR42_RS11485 at 2456.559 kb on - strand, within RR42_RS11485
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 2,453,659 + RR42_RS11465 0.22 +0.3 2,453,662 + RR42_RS11465 0.23 +0.0 2,453,663 - RR42_RS11465 0.23 +0.8 2,453,663 - RR42_RS11465 0.23 +1.2 2,453,663 - RR42_RS11465 0.23 +0.3 2,453,663 - RR42_RS11465 0.23 +1.5 2,453,887 + RR42_RS11465 0.55 +0.7 2,453,888 - RR42_RS11465 0.55 +1.2 2,453,888 - RR42_RS11465 0.55 +0.8 2,453,999 - RR42_RS11465 0.71 +4.0 2,454,422 - RR42_RS11470 0.83 +0.0 2,454,503 + -0.0 2,454,571 + RR42_RS11475 0.11 +0.2 2,454,731 - RR42_RS11475 0.25 +0.8 2,454,808 - RR42_RS11475 0.32 +1.9 2,454,815 + RR42_RS11475 0.32 +0.2 2,455,006 + RR42_RS11475 0.49 +0.3 2,455,007 - RR42_RS11475 0.49 +0.5 2,455,031 - RR42_RS11475 0.51 -0.1 2,455,034 - RR42_RS11475 0.51 +0.4 2,455,316 + RR42_RS11475 0.76 -0.2 2,455,317 - RR42_RS11475 0.76 -0.6 2,455,511 + -0.6 2,455,784 + +1.1 2,455,968 + RR42_RS11480 0.47 +0.7 2,455,969 - RR42_RS11480 0.48 +0.3 2,456,003 + RR42_RS11480 0.58 +0.1 2,456,155 + +0.3 2,456,156 - +1.3 2,456,156 - -0.5 2,456,159 + -0.6 2,456,160 - -0.9 2,456,238 + -0.5 2,456,238 + -0.6 2,456,238 + -0.0 2,456,238 + +0.4 2,456,239 - +1.8 2,456,239 - -1.3 2,456,243 + +0.2 2,456,243 + +1.1 2,456,243 + -1.0 2,456,244 - -0.3 2,456,384 + -0.0 2,456,385 - -0.5 2,456,385 - -0.5 2,456,489 + RR42_RS11485 0.13 -1.3 2,456,559 - RR42_RS11485 0.17 -0.4
Or see this region's nucleotide sequence