Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01410 and RR42_RS01415 are separated by 200 nucleotides RR42_RS01415 and RR42_RS01420 are separated by 8 nucleotides RR42_RS01420 and RR42_RS01425 are separated by 727 nucleotides RR42_RS01425 and RR42_RS01430 are separated by 38 nucleotides
RR42_RS01410: RR42_RS01410 - NAD-dependent dehydratase, at 318,260 to 319,285
_RS01410
RR42_RS01415: RR42_RS01415 - glutathione S-transferase, at 319,486 to 320,175
_RS01415
RR42_RS01420: RR42_RS01420 - 2', 3'-cyclic nucleotide 2'-phosphodiesterase, at 320,184 to 321,419
_RS01420
RR42_RS01425: RR42_RS01425 - hypothetical protein, at 322,147 to 322,341
_RS01425
RR42_RS01430: RR42_RS01430 - hypothetical protein, at 322,380 to 323,579
_RS01430
Position (kb) 320
321
322 Strain fitness (log2 ratio) -1
0
1
2 at 319.351 kb on - strand at 319.351 kb on - strand at 319.382 kb on + strand at 319.382 kb on + strand at 319.383 kb on - strand at 319.513 kb on + strand at 319.513 kb on + strand at 319.513 kb on + strand at 319.643 kb on + strand, within RR42_RS01415 at 319.643 kb on + strand, within RR42_RS01415 at 319.644 kb on - strand, within RR42_RS01415 at 319.699 kb on + strand, within RR42_RS01415 at 320.005 kb on + strand, within RR42_RS01415 at 320.005 kb on + strand, within RR42_RS01415 at 320.005 kb on + strand, within RR42_RS01415 at 320.005 kb on + strand, within RR42_RS01415 at 320.006 kb on - strand, within RR42_RS01415 at 320.038 kb on + strand, within RR42_RS01415 at 320.193 kb on + strand at 320.193 kb on + strand at 320.859 kb on + strand, within RR42_RS01420 at 321.005 kb on + strand, within RR42_RS01420 at 321.463 kb on - strand at 322.039 kb on + strand at 322.087 kb on + strand at 322.088 kb on - strand at 322.131 kb on - strand at 322.138 kb on + strand at 322.138 kb on + strand at 322.355 kb on + strand at 322.355 kb on + strand at 322.356 kb on - strand at 322.381 kb on + strand at 322.381 kb on + strand at 322.381 kb on + strand at 322.382 kb on - strand at 322.384 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 319,351 - -0.2 319,351 - -0.1 319,382 + +0.5 319,382 + -0.7 319,383 - -0.5 319,513 + +0.1 319,513 + +1.0 319,513 + +0.5 319,643 + RR42_RS01415 0.23 +0.1 319,643 + RR42_RS01415 0.23 +0.3 319,644 - RR42_RS01415 0.23 -0.4 319,699 + RR42_RS01415 0.31 -0.3 320,005 + RR42_RS01415 0.75 -0.1 320,005 + RR42_RS01415 0.75 -0.1 320,005 + RR42_RS01415 0.75 -1.3 320,005 + RR42_RS01415 0.75 -1.1 320,006 - RR42_RS01415 0.75 -0.1 320,038 + RR42_RS01415 0.80 -0.6 320,193 + +0.6 320,193 + -0.2 320,859 + RR42_RS01420 0.55 +0.3 321,005 + RR42_RS01420 0.66 +2.4 321,463 - -0.0 322,039 + -0.6 322,087 + -0.5 322,088 - -0.2 322,131 - +0.4 322,138 + +0.9 322,138 + +1.1 322,355 + +0.8 322,355 + -0.2 322,356 - -0.9 322,381 + -0.7 322,381 + +0.2 322,381 + +0.4 322,382 - -0.7 322,384 - -0.6
Or see this region's nucleotide sequence