Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00930 and RR42_RS00935 overlap by 4 nucleotides RR42_RS00935 and RR42_RS00940 are separated by 57 nucleotides
RR42_RS00930: RR42_RS00930 - 8-amino-7-oxononanoate synthase, at 222,278 to 223,495
_RS00930
RR42_RS00935: RR42_RS00935 - dethiobiotin synthetase, at 223,492 to 224,214
_RS00935
RR42_RS00940: RR42_RS00940 - biotin synthase, at 224,272 to 225,318
_RS00940
Position (kb) 224
225
226 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 224.252 kb on + strand at 224.410 kb on + strand, within RR42_RS00940 at 224.410 kb on + strand, within RR42_RS00940 at 224.810 kb on - strand, within RR42_RS00940 at 224.812 kb on + strand, within RR42_RS00940 at 224.812 kb on + strand, within RR42_RS00940 at 224.813 kb on - strand, within RR42_RS00940 at 224.813 kb on - strand, within RR42_RS00940 at 224.813 kb on - strand, within RR42_RS00940 at 224.829 kb on + strand, within RR42_RS00940 at 224.845 kb on + strand, within RR42_RS00940 at 224.846 kb on - strand, within RR42_RS00940 at 224.846 kb on - strand, within RR42_RS00940 at 224.872 kb on + strand, within RR42_RS00940 at 224.873 kb on - strand, within RR42_RS00940 at 224.873 kb on - strand, within RR42_RS00940 at 224.873 kb on - strand, within RR42_RS00940 at 225.220 kb on + strand at 225.307 kb on + strand at 225.308 kb on - strand at 225.509 kb on + strand at 225.509 kb on + strand at 225.619 kb on - strand at 225.789 kb on + strand at 225.789 kb on + strand at 225.789 kb on + strand at 225.789 kb on + strand at 225.790 kb on - strand at 225.790 kb on - strand at 225.853 kb on - strand at 226.163 kb on + strand at 226.220 kb on + strand at 226.221 kb on - strand at 226.231 kb on + strand at 226.231 kb on + strand at 226.231 kb on + strand at 226.231 kb on + strand at 226.232 kb on - strand at 226.232 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 224,252 + -0.4 224,410 + RR42_RS00940 0.13 -1.2 224,410 + RR42_RS00940 0.13 -1.9 224,810 - RR42_RS00940 0.51 -1.4 224,812 + RR42_RS00940 0.52 -1.0 224,812 + RR42_RS00940 0.52 -1.3 224,813 - RR42_RS00940 0.52 -1.9 224,813 - RR42_RS00940 0.52 -1.8 224,813 - RR42_RS00940 0.52 -2.6 224,829 + RR42_RS00940 0.53 -0.1 224,845 + RR42_RS00940 0.55 -1.7 224,846 - RR42_RS00940 0.55 -1.5 224,846 - RR42_RS00940 0.55 -0.8 224,872 + RR42_RS00940 0.57 -1.2 224,873 - RR42_RS00940 0.57 -2.4 224,873 - RR42_RS00940 0.57 +0.3 224,873 - RR42_RS00940 0.57 -2.3 225,220 + +0.3 225,307 + -0.3 225,308 - -0.8 225,509 + +0.2 225,509 + -0.4 225,619 - -0.1 225,789 + +0.1 225,789 + -0.6 225,789 + -0.2 225,789 + +0.8 225,790 - +0.8 225,790 - +0.4 225,853 - +3.5 226,163 + +0.2 226,220 + -1.0 226,221 - +1.7 226,231 + +0.3 226,231 + +0.1 226,231 + -0.3 226,231 + -0.5 226,232 - -1.2 226,232 - +0.8
Or see this region's nucleotide sequence