Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1543

Experiment: Chlorite 0.08 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1541 and Pf6N2E2_1542 overlap by 14 nucleotidesPf6N2E2_1542 and Pf6N2E2_1543 overlap by 4 nucleotidesPf6N2E2_1543 and Pf6N2E2_1544 overlap by 11 nucleotidesPf6N2E2_1544 and Pf6N2E2_1545 are separated by 199 nucleotides Pf6N2E2_1541 - Short-chain dehydrogenase/reductase SDR, at 1,799,771 to 1,800,580 _1541 Pf6N2E2_1542 - long-chain acyl-CoA synthetase, at 1,800,567 to 1,801,241 _1542 Pf6N2E2_1543 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,801,238 to 1,802,719 _1543 Pf6N2E2_1544 - Thermostable hemolysin delta-VPH, at 1,802,709 to 1,803,386 _1544 Pf6N2E2_1545 - N-carbamoylputrescine amidase (EC 3.5.1.53), at 1,803,586 to 1,805,337 _1545 Position (kb) 1801 1802 1803Strain fitness (log2 ratio) -2 -1 0 1at 1800.375 kb on - strand, within Pf6N2E2_1541at 1800.706 kb on + strand, within Pf6N2E2_1542at 1800.716 kb on + strand, within Pf6N2E2_1542at 1800.716 kb on + strand, within Pf6N2E2_1542at 1800.717 kb on - strand, within Pf6N2E2_1542at 1800.717 kb on - strand, within Pf6N2E2_1542at 1800.751 kb on + strand, within Pf6N2E2_1542at 1800.889 kb on + strand, within Pf6N2E2_1542at 1800.896 kb on - strand, within Pf6N2E2_1542at 1800.896 kb on - strand, within Pf6N2E2_1542at 1800.896 kb on - strand, within Pf6N2E2_1542at 1800.896 kb on - strand, within Pf6N2E2_1542at 1800.896 kb on - strand, within Pf6N2E2_1542at 1800.896 kb on - strand, within Pf6N2E2_1542at 1801.001 kb on - strand, within Pf6N2E2_1542at 1801.015 kb on + strand, within Pf6N2E2_1542at 1801.015 kb on + strand, within Pf6N2E2_1542at 1801.015 kb on + strand, within Pf6N2E2_1542at 1801.106 kb on - strand, within Pf6N2E2_1542at 1801.106 kb on - strand, within Pf6N2E2_1542at 1801.108 kb on + strand, within Pf6N2E2_1542at 1801.108 kb on + strand, within Pf6N2E2_1542at 1801.109 kb on - strand, within Pf6N2E2_1542at 1801.503 kb on + strand, within Pf6N2E2_1543at 1801.618 kb on - strand, within Pf6N2E2_1543at 1802.115 kb on + strand, within Pf6N2E2_1543at 1802.115 kb on + strand, within Pf6N2E2_1543at 1802.116 kb on - strand, within Pf6N2E2_1543at 1802.116 kb on - strand, within Pf6N2E2_1543at 1802.127 kb on + strand, within Pf6N2E2_1543at 1802.128 kb on - strand, within Pf6N2E2_1543at 1802.166 kb on - strand, within Pf6N2E2_1543at 1802.593 kb on - strandat 1802.607 kb on + strandat 1802.986 kb on + strand, within Pf6N2E2_1544at 1802.986 kb on + strand, within Pf6N2E2_1544at 1802.986 kb on + strand, within Pf6N2E2_1544at 1802.987 kb on - strand, within Pf6N2E2_1544at 1802.987 kb on - strand, within Pf6N2E2_1544at 1802.987 kb on - strand, within Pf6N2E2_1544at 1802.987 kb on - strand, within Pf6N2E2_1544at 1802.987 kb on - strand, within Pf6N2E2_1544at 1803.121 kb on + strand, within Pf6N2E2_1544at 1803.121 kb on + strand, within Pf6N2E2_1544at 1803.121 kb on + strand, within Pf6N2E2_1544at 1803.422 kb on + strandat 1803.423 kb on - strandat 1803.423 kb on - strandat 1803.439 kb on - strandat 1803.523 kb on + strandat 1803.523 kb on + strandat 1803.718 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chlorite 0.08 mM
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1,800,375 - Pf6N2E2_1541 0.75 -0.3
1,800,706 + Pf6N2E2_1542 0.21 +0.4
1,800,716 + Pf6N2E2_1542 0.22 -2.6
1,800,716 + Pf6N2E2_1542 0.22 -0.3
1,800,717 - Pf6N2E2_1542 0.22 +0.1
1,800,717 - Pf6N2E2_1542 0.22 +0.1
1,800,751 + Pf6N2E2_1542 0.27 -1.3
1,800,889 + Pf6N2E2_1542 0.48 -0.3
1,800,896 - Pf6N2E2_1542 0.49 -1.1
1,800,896 - Pf6N2E2_1542 0.49 -0.4
1,800,896 - Pf6N2E2_1542 0.49 +0.0
1,800,896 - Pf6N2E2_1542 0.49 +0.3
1,800,896 - Pf6N2E2_1542 0.49 +0.7
1,800,896 - Pf6N2E2_1542 0.49 +0.4
1,801,001 - Pf6N2E2_1542 0.64 -0.2
1,801,015 + Pf6N2E2_1542 0.66 +0.9
1,801,015 + Pf6N2E2_1542 0.66 -0.0
1,801,015 + Pf6N2E2_1542 0.66 -0.6
1,801,106 - Pf6N2E2_1542 0.80 +0.4
1,801,106 - Pf6N2E2_1542 0.80 -0.2
1,801,108 + Pf6N2E2_1542 0.80 +0.3
1,801,108 + Pf6N2E2_1542 0.80 -0.2
1,801,109 - Pf6N2E2_1542 0.80 -0.4
1,801,503 + Pf6N2E2_1543 0.18 -0.3
1,801,618 - Pf6N2E2_1543 0.26 -0.7
1,802,115 + Pf6N2E2_1543 0.59 -1.0
1,802,115 + Pf6N2E2_1543 0.59 -0.4
1,802,116 - Pf6N2E2_1543 0.59 -1.4
1,802,116 - Pf6N2E2_1543 0.59 -1.0
1,802,127 + Pf6N2E2_1543 0.60 -0.1
1,802,128 - Pf6N2E2_1543 0.60 +0.0
1,802,166 - Pf6N2E2_1543 0.63 +0.2
1,802,593 - -0.6
1,802,607 + +0.3
1,802,986 + Pf6N2E2_1544 0.41 +0.5
1,802,986 + Pf6N2E2_1544 0.41 -0.5
1,802,986 + Pf6N2E2_1544 0.41 -0.5
1,802,987 - Pf6N2E2_1544 0.41 +0.5
1,802,987 - Pf6N2E2_1544 0.41 -0.9
1,802,987 - Pf6N2E2_1544 0.41 -0.5
1,802,987 - Pf6N2E2_1544 0.41 +0.4
1,802,987 - Pf6N2E2_1544 0.41 +0.1
1,803,121 + Pf6N2E2_1544 0.61 -0.9
1,803,121 + Pf6N2E2_1544 0.61 +1.6
1,803,121 + Pf6N2E2_1544 0.61 -0.2
1,803,422 + +0.5
1,803,423 - -0.2
1,803,423 - +0.6
1,803,439 - +0.7
1,803,523 + -0.5
1,803,523 + -1.2
1,803,718 + -1.3

Or see this region's nucleotide sequence