Experiment: R2A with Paraquat dichloride 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15825 and CA265_RS15830 are separated by 107 nucleotides CA265_RS15830 and CA265_RS15835 are separated by 32 nucleotides CA265_RS15835 and CA265_RS15840 are separated by 80 nucleotides
CA265_RS15825: CA265_RS15825 - ketol-acid reductoisomerase, at 3,981,839 to 3,983,320
_RS15825
CA265_RS15830: CA265_RS15830 - 3-isopropylmalate dehydratase large subunit, at 3,983,428 to 3,984,840
_RS15830
CA265_RS15835: CA265_RS15835 - antibiotic biosynthesis monooxygenase, at 3,984,873 to 3,985,217
_RS15835
CA265_RS15840: CA265_RS15840 - 3-isopropylmalate dehydratase small subunit, at 3,985,298 to 3,985,885
_RS15840
Position (kb)
3983
3984
3985 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3982.430 kb on - strand, within CA265_RS15825 at 3982.525 kb on - strand, within CA265_RS15825 at 3982.542 kb on + strand, within CA265_RS15825 at 3982.553 kb on - strand, within CA265_RS15825 at 3982.561 kb on - strand, within CA265_RS15825 at 3982.605 kb on - strand, within CA265_RS15825 at 3982.605 kb on - strand, within CA265_RS15825 at 3982.617 kb on + strand, within CA265_RS15825 at 3982.646 kb on - strand, within CA265_RS15825 at 3982.760 kb on - strand, within CA265_RS15825 at 3982.760 kb on - strand, within CA265_RS15825 at 3982.760 kb on - strand, within CA265_RS15825 at 3982.917 kb on + strand, within CA265_RS15825 at 3983.084 kb on - strand, within CA265_RS15825 at 3983.094 kb on - strand, within CA265_RS15825 at 3983.094 kb on - strand, within CA265_RS15825 at 3983.102 kb on + strand, within CA265_RS15825 at 3983.106 kb on + strand, within CA265_RS15825 at 3983.387 kb on + strand at 3983.395 kb on + strand at 3983.523 kb on - strand at 3983.621 kb on + strand, within CA265_RS15830 at 3983.622 kb on - strand, within CA265_RS15830 at 3983.679 kb on + strand, within CA265_RS15830 at 3983.720 kb on - strand, within CA265_RS15830 at 3983.982 kb on - strand, within CA265_RS15830 at 3983.982 kb on - strand, within CA265_RS15830 at 3983.988 kb on + strand, within CA265_RS15830 at 3984.023 kb on - strand, within CA265_RS15830 at 3984.060 kb on - strand, within CA265_RS15830 at 3984.088 kb on - strand, within CA265_RS15830 at 3984.221 kb on + strand, within CA265_RS15830 at 3984.224 kb on - strand, within CA265_RS15830 at 3984.295 kb on + strand, within CA265_RS15830 at 3984.296 kb on - strand, within CA265_RS15830 at 3984.299 kb on + strand, within CA265_RS15830 at 3984.337 kb on - strand, within CA265_RS15830 at 3984.398 kb on - strand, within CA265_RS15830 at 3984.446 kb on - strand, within CA265_RS15830 at 3984.673 kb on - strand, within CA265_RS15830 at 3984.748 kb on - strand at 3984.831 kb on - strand at 3984.866 kb on + strand at 3984.879 kb on - strand at 3984.943 kb on + strand, within CA265_RS15835 at 3984.944 kb on - strand, within CA265_RS15835 at 3984.946 kb on + strand, within CA265_RS15835 at 3984.946 kb on + strand, within CA265_RS15835 at 3985.067 kb on + strand, within CA265_RS15835 at 3985.072 kb on - strand, within CA265_RS15835 at 3985.073 kb on + strand, within CA265_RS15835 at 3985.138 kb on - strand, within CA265_RS15835 at 3985.160 kb on - strand, within CA265_RS15835 at 3985.171 kb on - strand, within CA265_RS15835 at 3985.226 kb on - strand at 3985.228 kb on - strand at 3985.272 kb on + strand at 3985.370 kb on - strand, within CA265_RS15840 at 3985.480 kb on + strand, within CA265_RS15840 at 3985.487 kb on - strand, within CA265_RS15840 at 3985.538 kb on - strand, within CA265_RS15840 at 3985.612 kb on - strand, within CA265_RS15840 at 3985.657 kb on - strand, within CA265_RS15840 at 3985.736 kb on - strand, within CA265_RS15840 at 3985.745 kb on - strand, within CA265_RS15840 at 3985.818 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.001 mg/ml remove 3,982,430 - CA265_RS15825 0.40 -2.1 3,982,525 - CA265_RS15825 0.46 -0.4 3,982,542 + CA265_RS15825 0.47 -0.8 3,982,553 - CA265_RS15825 0.48 -1.1 3,982,561 - CA265_RS15825 0.49 -1.1 3,982,605 - CA265_RS15825 0.52 -1.7 3,982,605 - CA265_RS15825 0.52 -1.4 3,982,617 + CA265_RS15825 0.52 -0.7 3,982,646 - CA265_RS15825 0.54 -0.4 3,982,760 - CA265_RS15825 0.62 -2.1 3,982,760 - CA265_RS15825 0.62 -1.9 3,982,760 - CA265_RS15825 0.62 +0.5 3,982,917 + CA265_RS15825 0.73 +0.8 3,983,084 - CA265_RS15825 0.84 -1.0 3,983,094 - CA265_RS15825 0.85 -1.3 3,983,094 - CA265_RS15825 0.85 -0.1 3,983,102 + CA265_RS15825 0.85 -0.9 3,983,106 + CA265_RS15825 0.85 -0.0 3,983,387 + -1.0 3,983,395 + -0.2 3,983,523 - -0.9 3,983,621 + CA265_RS15830 0.14 -0.0 3,983,622 - CA265_RS15830 0.14 -0.8 3,983,679 + CA265_RS15830 0.18 -0.9 3,983,720 - CA265_RS15830 0.21 -0.5 3,983,982 - CA265_RS15830 0.39 +1.0 3,983,982 - CA265_RS15830 0.39 -1.3 3,983,988 + CA265_RS15830 0.40 -1.7 3,984,023 - CA265_RS15830 0.42 -1.0 3,984,060 - CA265_RS15830 0.45 -2.1 3,984,088 - CA265_RS15830 0.47 -1.8 3,984,221 + CA265_RS15830 0.56 -0.1 3,984,224 - CA265_RS15830 0.56 -1.4 3,984,295 + CA265_RS15830 0.61 -0.5 3,984,296 - CA265_RS15830 0.61 -1.0 3,984,299 + CA265_RS15830 0.62 -1.3 3,984,337 - CA265_RS15830 0.64 -0.7 3,984,398 - CA265_RS15830 0.69 -2.5 3,984,446 - CA265_RS15830 0.72 -1.4 3,984,673 - CA265_RS15830 0.88 -1.0 3,984,748 - -0.6 3,984,831 - -1.6 3,984,866 + -2.1 3,984,879 - -2.4 3,984,943 + CA265_RS15835 0.20 +0.5 3,984,944 - CA265_RS15835 0.21 -1.0 3,984,946 + CA265_RS15835 0.21 -0.7 3,984,946 + CA265_RS15835 0.21 -0.4 3,985,067 + CA265_RS15835 0.56 +3.1 3,985,072 - CA265_RS15835 0.58 -0.3 3,985,073 + CA265_RS15835 0.58 +0.0 3,985,138 - CA265_RS15835 0.77 -1.9 3,985,160 - CA265_RS15835 0.83 -0.8 3,985,171 - CA265_RS15835 0.86 -1.0 3,985,226 - -0.8 3,985,228 - +0.2 3,985,272 + -0.7 3,985,370 - CA265_RS15840 0.12 -0.6 3,985,480 + CA265_RS15840 0.31 -0.0 3,985,487 - CA265_RS15840 0.32 -1.2 3,985,538 - CA265_RS15840 0.41 -1.7 3,985,612 - CA265_RS15840 0.53 -0.9 3,985,657 - CA265_RS15840 0.61 -2.4 3,985,736 - CA265_RS15840 0.74 -0.3 3,985,745 - CA265_RS15840 0.76 -1.5 3,985,818 - -0.0
Or see this region's nucleotide sequence