Experiment: R2A with Paraquat dichloride 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04395 and CA265_RS04400 are separated by 109 nucleotides CA265_RS04400 and CA265_RS04405 are separated by 176 nucleotides
CA265_RS04395: CA265_RS04395 - heptosyltransferase, at 1,054,767 to 1,055,798
_RS04395
CA265_RS04400: CA265_RS04400 - hypothetical protein, at 1,055,908 to 1,056,510
_RS04400
CA265_RS04405: CA265_RS04405 - DNA polymerase/3'-5' exonuclease PolX, at 1,056,687 to 1,058,363
_RS04405
Position (kb)
1055
1056
1057 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1054.942 kb on + strand, within CA265_RS04395 at 1055.018 kb on + strand, within CA265_RS04395 at 1055.023 kb on - strand, within CA265_RS04395 at 1055.027 kb on + strand, within CA265_RS04395 at 1055.028 kb on + strand, within CA265_RS04395 at 1055.028 kb on + strand, within CA265_RS04395 at 1055.029 kb on - strand, within CA265_RS04395 at 1055.253 kb on - strand, within CA265_RS04395 at 1055.253 kb on - strand, within CA265_RS04395 at 1055.276 kb on - strand, within CA265_RS04395 at 1055.348 kb on + strand, within CA265_RS04395 at 1055.349 kb on - strand, within CA265_RS04395 at 1055.460 kb on + strand, within CA265_RS04395 at 1055.460 kb on + strand, within CA265_RS04395 at 1055.461 kb on - strand, within CA265_RS04395 at 1055.582 kb on - strand, within CA265_RS04395 at 1055.606 kb on + strand, within CA265_RS04395 at 1055.645 kb on + strand, within CA265_RS04395 at 1055.769 kb on + strand at 1055.848 kb on + strand at 1055.848 kb on + strand at 1055.870 kb on + strand at 1055.903 kb on + strand at 1055.906 kb on + strand at 1055.907 kb on - strand at 1055.909 kb on + strand at 1055.910 kb on - strand at 1055.916 kb on + strand at 1055.919 kb on + strand at 1055.921 kb on + strand at 1055.922 kb on - strand at 1055.968 kb on - strand at 1055.971 kb on + strand, within CA265_RS04400 at 1056.052 kb on + strand, within CA265_RS04400 at 1056.055 kb on + strand, within CA265_RS04400 at 1056.056 kb on - strand, within CA265_RS04400 at 1056.126 kb on + strand, within CA265_RS04400 at 1056.126 kb on + strand, within CA265_RS04400 at 1056.150 kb on + strand, within CA265_RS04400 at 1056.163 kb on + strand, within CA265_RS04400 at 1056.240 kb on - strand, within CA265_RS04400 at 1056.290 kb on - strand, within CA265_RS04400 at 1056.336 kb on + strand, within CA265_RS04400 at 1056.340 kb on + strand, within CA265_RS04400 at 1056.341 kb on - strand, within CA265_RS04400 at 1056.341 kb on - strand, within CA265_RS04400 at 1056.462 kb on - strand at 1056.468 kb on + strand at 1056.522 kb on - strand at 1056.629 kb on + strand at 1056.698 kb on + strand at 1056.698 kb on + strand at 1056.698 kb on + strand at 1056.709 kb on + strand at 1056.718 kb on - strand at 1056.759 kb on + strand at 1056.780 kb on + strand at 1056.815 kb on + strand at 1056.846 kb on + strand at 1056.846 kb on + strand at 1056.847 kb on - strand at 1056.847 kb on - strand at 1056.847 kb on - strand at 1056.987 kb on + strand, within CA265_RS04405 at 1056.993 kb on + strand, within CA265_RS04405 at 1057.014 kb on + strand, within CA265_RS04405 at 1057.025 kb on + strand, within CA265_RS04405 at 1057.025 kb on + strand, within CA265_RS04405 at 1057.026 kb on - strand, within CA265_RS04405 at 1057.027 kb on + strand, within CA265_RS04405 at 1057.131 kb on - strand, within CA265_RS04405 at 1057.260 kb on + strand, within CA265_RS04405 at 1057.261 kb on - strand, within CA265_RS04405 at 1057.287 kb on - strand, within CA265_RS04405 at 1057.305 kb on + strand, within CA265_RS04405 at 1057.320 kb on + strand, within CA265_RS04405 at 1057.336 kb on + strand, within CA265_RS04405 at 1057.359 kb on - strand, within CA265_RS04405 at 1057.359 kb on - strand, within CA265_RS04405 at 1057.359 kb on - strand, within CA265_RS04405 at 1057.362 kb on + strand, within CA265_RS04405 at 1057.363 kb on - strand, within CA265_RS04405 at 1057.415 kb on - strand, within CA265_RS04405
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.001 mg/ml remove 1,054,942 + CA265_RS04395 0.17 -1.5 1,055,018 + CA265_RS04395 0.24 -0.7 1,055,023 - CA265_RS04395 0.25 -0.8 1,055,027 + CA265_RS04395 0.25 -0.2 1,055,028 + CA265_RS04395 0.25 -0.9 1,055,028 + CA265_RS04395 0.25 -0.4 1,055,029 - CA265_RS04395 0.25 -1.1 1,055,253 - CA265_RS04395 0.47 -1.0 1,055,253 - CA265_RS04395 0.47 -0.4 1,055,276 - CA265_RS04395 0.49 -0.1 1,055,348 + CA265_RS04395 0.56 -2.6 1,055,349 - CA265_RS04395 0.56 -0.0 1,055,460 + CA265_RS04395 0.67 -2.1 1,055,460 + CA265_RS04395 0.67 +1.1 1,055,461 - CA265_RS04395 0.67 -0.3 1,055,582 - CA265_RS04395 0.79 -1.2 1,055,606 + CA265_RS04395 0.81 -0.8 1,055,645 + CA265_RS04395 0.85 -1.3 1,055,769 + -1.4 1,055,848 + +0.4 1,055,848 + +1.2 1,055,870 + +0.7 1,055,903 + +0.6 1,055,906 + +0.5 1,055,907 - +0.1 1,055,909 + +0.7 1,055,910 - -0.5 1,055,916 + +0.1 1,055,919 + +0.9 1,055,921 + -0.5 1,055,922 - -0.8 1,055,968 - -1.2 1,055,971 + CA265_RS04400 0.10 -0.9 1,056,052 + CA265_RS04400 0.24 -1.2 1,056,055 + CA265_RS04400 0.24 -0.7 1,056,056 - CA265_RS04400 0.25 -0.6 1,056,126 + CA265_RS04400 0.36 -0.8 1,056,126 + CA265_RS04400 0.36 -0.4 1,056,150 + CA265_RS04400 0.40 -1.8 1,056,163 + CA265_RS04400 0.42 -2.6 1,056,240 - CA265_RS04400 0.55 -0.1 1,056,290 - CA265_RS04400 0.63 +0.7 1,056,336 + CA265_RS04400 0.71 -0.8 1,056,340 + CA265_RS04400 0.72 -1.3 1,056,341 - CA265_RS04400 0.72 -1.1 1,056,341 - CA265_RS04400 0.72 -1.0 1,056,462 - -0.1 1,056,468 + -1.5 1,056,522 - +0.1 1,056,629 + +0.1 1,056,698 + -0.1 1,056,698 + -0.5 1,056,698 + -0.3 1,056,709 + -0.5 1,056,718 - +0.3 1,056,759 + -1.3 1,056,780 + -0.1 1,056,815 + +0.3 1,056,846 + +0.0 1,056,846 + -2.1 1,056,847 - -0.2 1,056,847 - +0.9 1,056,847 - -0.8 1,056,987 + CA265_RS04405 0.18 +0.2 1,056,993 + CA265_RS04405 0.18 +0.1 1,057,014 + CA265_RS04405 0.19 +0.3 1,057,025 + CA265_RS04405 0.20 -0.6 1,057,025 + CA265_RS04405 0.20 -0.3 1,057,026 - CA265_RS04405 0.20 -1.1 1,057,027 + CA265_RS04405 0.20 +0.0 1,057,131 - CA265_RS04405 0.26 +0.0 1,057,260 + CA265_RS04405 0.34 -0.8 1,057,261 - CA265_RS04405 0.34 -0.5 1,057,287 - CA265_RS04405 0.36 +0.8 1,057,305 + CA265_RS04405 0.37 +0.1 1,057,320 + CA265_RS04405 0.38 +2.0 1,057,336 + CA265_RS04405 0.39 +0.2 1,057,359 - CA265_RS04405 0.40 +0.9 1,057,359 - CA265_RS04405 0.40 +0.2 1,057,359 - CA265_RS04405 0.40 -0.6 1,057,362 + CA265_RS04405 0.40 -0.0 1,057,363 - CA265_RS04405 0.40 -1.8 1,057,415 - CA265_RS04405 0.43 +1.0
Or see this region's nucleotide sequence