Experiment: Bacitracin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24570 and BPHYT_RS24575 are separated by 17 nucleotides BPHYT_RS24575 and BPHYT_RS24580 are separated by 3 nucleotides BPHYT_RS24580 and BPHYT_RS24585 are separated by 222 nucleotides BPHYT_RS24585 and BPHYT_RS24590 are separated by 116 nucleotides
BPHYT_RS24570: BPHYT_RS24570 - membrane protein, at 1,082,890 to 1,084,050
_RS24570
BPHYT_RS24575: BPHYT_RS24575 - hypothetical protein, at 1,084,068 to 1,084,268
_RS24575
BPHYT_RS24580: BPHYT_RS24580 - hypothetical protein, at 1,084,272 to 1,084,601
_RS24580
BPHYT_RS24585: BPHYT_RS24585 - NUDIX hydrolase, at 1,084,824 to 1,085,411
_RS24585
BPHYT_RS24590: BPHYT_RS24590 - DeoR faimly transcriptional regulator, at 1,085,528 to 1,086,289
_RS24590
Position (kb) 1084
1085
1086 Strain fitness (log2 ratio) -1
0
1
2
3 at 1083.883 kb on + strand, within BPHYT_RS24570 at 1084.202 kb on + strand, within BPHYT_RS24575 at 1084.242 kb on - strand at 1084.359 kb on - strand, within BPHYT_RS24580 at 1084.737 kb on - strand at 1084.742 kb on + strand at 1084.999 kb on + strand, within BPHYT_RS24585 at 1086.098 kb on + strand, within BPHYT_RS24590 at 1086.236 kb on - strand at 1086.333 kb on + strand at 1086.333 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.125 mg/ml remove 1,083,883 + BPHYT_RS24570 0.86 +0.2 1,084,202 + BPHYT_RS24575 0.67 -0.0 1,084,242 - +3.1 1,084,359 - BPHYT_RS24580 0.26 -0.1 1,084,737 - +0.2 1,084,742 + -0.3 1,084,999 + BPHYT_RS24585 0.30 -1.4 1,086,098 + BPHYT_RS24590 0.75 -0.5 1,086,236 - -0.1 1,086,333 + -0.4 1,086,333 + -0.2
Or see this region's nucleotide sequence