Experiment: Bacitracin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23220 and BPHYT_RS23225 are separated by 113 nucleotides BPHYT_RS23225 and BPHYT_RS23230 are separated by 30 nucleotides BPHYT_RS23230 and BPHYT_RS23235 are separated by 185 nucleotides BPHYT_RS23235 and BPHYT_RS23240 are separated by 350 nucleotides
BPHYT_RS23220: BPHYT_RS23220 - hypothetical protein, at 774,648 to 775,379
_RS23220
BPHYT_RS23225: BPHYT_RS23225 - hypothetical protein, at 775,493 to 775,699
_RS23225
BPHYT_RS23230: BPHYT_RS23230 - histidine kinase, at 775,730 to 777,703
_RS23230
BPHYT_RS23235: BPHYT_RS23235 - hypothetical protein, at 777,889 to 778,272
_RS23235
BPHYT_RS23240: BPHYT_RS23240 - DNA-binding protein, at 778,623 to 778,901
_RS23240
Position (kb) 775
776
777
778 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 774.741 kb on + strand, within BPHYT_RS23220 at 775.111 kb on - strand, within BPHYT_RS23220 at 775.313 kb on + strand at 775.608 kb on - strand, within BPHYT_RS23225 at 775.610 kb on + strand, within BPHYT_RS23225 at 775.752 kb on + strand at 776.257 kb on - strand, within BPHYT_RS23230 at 777.315 kb on + strand, within BPHYT_RS23230 at 777.714 kb on + strand at 777.714 kb on + strand at 777.784 kb on - strand at 778.296 kb on + strand at 778.301 kb on - strand at 778.323 kb on - strand at 778.345 kb on + strand at 778.431 kb on + strand at 778.431 kb on + strand at 778.467 kb on - strand at 778.514 kb on + strand at 778.514 kb on + strand at 778.515 kb on - strand at 778.523 kb on + strand at 778.559 kb on + strand at 778.605 kb on + strand at 778.611 kb on - strand at 778.622 kb on + strand at 778.623 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.125 mg/ml remove 774,741 + BPHYT_RS23220 0.13 +0.1 775,111 - BPHYT_RS23220 0.63 -0.1 775,313 + -0.0 775,608 - BPHYT_RS23225 0.56 +0.3 775,610 + BPHYT_RS23225 0.57 -0.8 775,752 + +1.3 776,257 - BPHYT_RS23230 0.27 -2.4 777,315 + BPHYT_RS23230 0.80 +0.5 777,714 + -0.1 777,714 + -3.5 777,784 - -0.2 778,296 + +0.2 778,301 - -0.6 778,323 - +0.8 778,345 + +0.2 778,431 + +0.0 778,431 + +0.0 778,467 - -0.2 778,514 + +0.3 778,514 + +0.2 778,515 - +0.0 778,523 + -0.2 778,559 + -0.3 778,605 + +0.7 778,611 - -0.7 778,622 + -0.2 778,623 - +1.1
Or see this region's nucleotide sequence