Experiment: Bacitracin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21930 and BPHYT_RS21935 are separated by 81 nucleotides BPHYT_RS21935 and BPHYT_RS21940 are separated by 165 nucleotides BPHYT_RS21940 and BPHYT_RS21945 are separated by 7 nucleotides
BPHYT_RS21930: BPHYT_RS21930 - MarR family transcriptional regulator, at 477,266 to 477,688
_RS21930
BPHYT_RS21935: BPHYT_RS21935 - MarR family transcriptional regulator, at 477,770 to 478,192
_RS21935
BPHYT_RS21940: BPHYT_RS21940 - hemolysin D, at 478,358 to 479,383
_RS21940
BPHYT_RS21945: BPHYT_RS21945 - DSBA oxidoreductase, at 479,391 to 480,962
_RS21945
Position (kb) 478
479
480 Strain fitness (log2 ratio) -2
-1
0
1
2 at 477.835 kb on - strand, within BPHYT_RS21935 at 477.835 kb on - strand, within BPHYT_RS21935 at 477.835 kb on - strand, within BPHYT_RS21935 at 477.836 kb on + strand, within BPHYT_RS21935 at 478.274 kb on - strand at 478.292 kb on - strand at 478.455 kb on - strand at 478.745 kb on + strand, within BPHYT_RS21940 at 478.961 kb on + strand, within BPHYT_RS21940 at 478.992 kb on - strand, within BPHYT_RS21940 at 479.964 kb on + strand, within BPHYT_RS21945 at 479.964 kb on + strand, within BPHYT_RS21945 at 479.965 kb on - strand, within BPHYT_RS21945 at 480.029 kb on + strand, within BPHYT_RS21945
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.125 mg/ml remove 477,835 - BPHYT_RS21935 0.15 +0.9 477,835 - BPHYT_RS21935 0.15 -0.3 477,835 - BPHYT_RS21935 0.15 -2.3 477,836 + BPHYT_RS21935 0.16 +0.3 478,274 - +2.3 478,292 - +0.0 478,455 - -0.2 478,745 + BPHYT_RS21940 0.38 -1.2 478,961 + BPHYT_RS21940 0.59 -0.6 478,992 - BPHYT_RS21940 0.62 -0.7 479,964 + BPHYT_RS21945 0.36 -0.0 479,964 + BPHYT_RS21945 0.36 -0.3 479,965 - BPHYT_RS21945 0.37 -0.6 480,029 + BPHYT_RS21945 0.41 -0.9
Or see this region's nucleotide sequence