Experiment: Bacitracin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18250 and BPHYT_RS18255 are separated by 38 nucleotides BPHYT_RS18255 and BPHYT_RS18260 are separated by 8 nucleotides BPHYT_RS18260 and BPHYT_RS18265 are separated by 7 nucleotides
BPHYT_RS18250: BPHYT_RS18250 - phenylacetate-CoA oxygenase subunit PaaA, at 4,105,416 to 4,106,414
_RS18250
BPHYT_RS18255: BPHYT_RS18255 - phenylacetate-CoA oxygenase subunit PaaB, at 4,106,453 to 4,106,737
_RS18255
BPHYT_RS18260: BPHYT_RS18260 - phenylacetic acid degradation protein, at 4,106,746 to 4,107,549
_RS18260
BPHYT_RS18265: BPHYT_RS18265 - phenylacetate-CoA oxygenase subunit PaaJ, at 4,107,557 to 4,108,108
_RS18265
Position (kb)
4106
4107 Strain fitness (log2 ratio)
-2
-1
0
1 at 4105.707 kb on + strand, within BPHYT_RS18250 at 4105.711 kb on + strand, within BPHYT_RS18250 at 4105.711 kb on + strand, within BPHYT_RS18250 at 4105.712 kb on - strand, within BPHYT_RS18250 at 4105.713 kb on + strand, within BPHYT_RS18250 at 4105.715 kb on + strand, within BPHYT_RS18250 at 4105.785 kb on + strand, within BPHYT_RS18250 at 4105.785 kb on + strand, within BPHYT_RS18250 at 4105.786 kb on - strand, within BPHYT_RS18250 at 4105.803 kb on + strand, within BPHYT_RS18250 at 4105.896 kb on + strand, within BPHYT_RS18250 at 4106.423 kb on + strand at 4106.491 kb on + strand, within BPHYT_RS18255 at 4106.576 kb on + strand, within BPHYT_RS18255 at 4106.577 kb on - strand, within BPHYT_RS18255 at 4106.687 kb on + strand, within BPHYT_RS18255 at 4106.735 kb on + strand at 4106.735 kb on + strand at 4106.736 kb on - strand at 4106.736 kb on - strand at 4106.971 kb on + strand, within BPHYT_RS18260 at 4106.971 kb on + strand, within BPHYT_RS18260 at 4107.007 kb on + strand, within BPHYT_RS18260 at 4107.007 kb on + strand, within BPHYT_RS18260 at 4107.007 kb on + strand, within BPHYT_RS18260 at 4107.101 kb on - strand, within BPHYT_RS18260 at 4107.101 kb on - strand, within BPHYT_RS18260 at 4107.196 kb on + strand, within BPHYT_RS18260 at 4107.196 kb on + strand, within BPHYT_RS18260 at 4107.196 kb on + strand, within BPHYT_RS18260 at 4107.196 kb on + strand, within BPHYT_RS18260 at 4107.197 kb on - strand, within BPHYT_RS18260 at 4107.283 kb on + strand, within BPHYT_RS18260 at 4107.283 kb on + strand, within BPHYT_RS18260 at 4107.547 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.125 mg/ml remove 4,105,707 + BPHYT_RS18250 0.29 -0.5 4,105,711 + BPHYT_RS18250 0.30 -0.7 4,105,711 + BPHYT_RS18250 0.30 -0.5 4,105,712 - BPHYT_RS18250 0.30 -1.4 4,105,713 + BPHYT_RS18250 0.30 -2.6 4,105,715 + BPHYT_RS18250 0.30 +0.1 4,105,785 + BPHYT_RS18250 0.37 -0.3 4,105,785 + BPHYT_RS18250 0.37 -0.3 4,105,786 - BPHYT_RS18250 0.37 -1.0 4,105,803 + BPHYT_RS18250 0.39 -1.4 4,105,896 + BPHYT_RS18250 0.48 -0.5 4,106,423 + -0.1 4,106,491 + BPHYT_RS18255 0.13 -0.1 4,106,576 + BPHYT_RS18255 0.43 -0.9 4,106,577 - BPHYT_RS18255 0.44 -1.3 4,106,687 + BPHYT_RS18255 0.82 +0.3 4,106,735 + -0.5 4,106,735 + -0.4 4,106,736 - +0.1 4,106,736 - +0.5 4,106,971 + BPHYT_RS18260 0.28 -1.0 4,106,971 + BPHYT_RS18260 0.28 -1.5 4,107,007 + BPHYT_RS18260 0.32 -1.0 4,107,007 + BPHYT_RS18260 0.32 -1.0 4,107,007 + BPHYT_RS18260 0.32 -0.5 4,107,101 - BPHYT_RS18260 0.44 -1.6 4,107,101 - BPHYT_RS18260 0.44 -0.2 4,107,196 + BPHYT_RS18260 0.56 -1.7 4,107,196 + BPHYT_RS18260 0.56 -0.7 4,107,196 + BPHYT_RS18260 0.56 -0.6 4,107,196 + BPHYT_RS18260 0.56 +0.4 4,107,197 - BPHYT_RS18260 0.56 -2.0 4,107,283 + BPHYT_RS18260 0.67 -0.4 4,107,283 + BPHYT_RS18260 0.67 -0.8 4,107,547 + -1.9
Or see this region's nucleotide sequence