Experiment: Bacitracin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14255 and BPHYT_RS14260 are separated by 110 nucleotides BPHYT_RS14260 and BPHYT_RS14265 are separated by 55 nucleotides
BPHYT_RS14255: BPHYT_RS14255 - ABC transporter, at 3,257,286 to 3,259,121
_RS14255
BPHYT_RS14260: BPHYT_RS14260 - trehalose-6-phosphate synthase, at 3,259,232 to 3,260,692
_RS14260
BPHYT_RS14265: BPHYT_RS14265 - trehalose-phosphatase, at 3,260,748 to 3,261,497
_RS14265
Position (kb)
3259
3260
3261 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 3258.277 kb on + strand, within BPHYT_RS14255 at 3258.419 kb on + strand, within BPHYT_RS14255 at 3258.501 kb on + strand, within BPHYT_RS14255 at 3258.600 kb on - strand, within BPHYT_RS14255 at 3258.625 kb on - strand, within BPHYT_RS14255 at 3258.729 kb on + strand, within BPHYT_RS14255 at 3259.060 kb on - strand at 3259.233 kb on + strand at 3259.563 kb on + strand, within BPHYT_RS14260 at 3259.563 kb on + strand, within BPHYT_RS14260 at 3259.564 kb on - strand, within BPHYT_RS14260 at 3259.564 kb on - strand, within BPHYT_RS14260 at 3259.564 kb on - strand, within BPHYT_RS14260 at 3259.608 kb on + strand, within BPHYT_RS14260 at 3259.656 kb on + strand, within BPHYT_RS14260 at 3259.671 kb on + strand, within BPHYT_RS14260 at 3259.671 kb on + strand, within BPHYT_RS14260 at 3259.672 kb on - strand, within BPHYT_RS14260 at 3259.672 kb on - strand, within BPHYT_RS14260 at 3259.672 kb on - strand, within BPHYT_RS14260 at 3259.672 kb on - strand, within BPHYT_RS14260 at 3259.756 kb on - strand, within BPHYT_RS14260 at 3260.224 kb on + strand, within BPHYT_RS14260 at 3260.225 kb on - strand, within BPHYT_RS14260 at 3260.295 kb on + strand, within BPHYT_RS14260 at 3260.295 kb on + strand, within BPHYT_RS14260 at 3260.295 kb on + strand, within BPHYT_RS14260 at 3260.295 kb on + strand, within BPHYT_RS14260 at 3260.397 kb on + strand, within BPHYT_RS14260 at 3260.397 kb on + strand, within BPHYT_RS14260 at 3260.397 kb on + strand, within BPHYT_RS14260 at 3260.447 kb on - strand, within BPHYT_RS14260 at 3260.460 kb on + strand, within BPHYT_RS14260 at 3260.463 kb on + strand, within BPHYT_RS14260 at 3260.463 kb on + strand, within BPHYT_RS14260 at 3260.463 kb on + strand, within BPHYT_RS14260 at 3260.464 kb on - strand, within BPHYT_RS14260 at 3260.464 kb on - strand, within BPHYT_RS14260 at 3260.464 kb on - strand, within BPHYT_RS14260 at 3260.608 kb on - strand at 3260.714 kb on + strand at 3260.715 kb on - strand at 3260.819 kb on + strand at 3260.819 kb on + strand at 3261.192 kb on + strand, within BPHYT_RS14265 at 3261.578 kb on + strand at 3261.578 kb on + strand at 3261.579 kb on - strand at 3261.579 kb on - strand at 3261.579 kb on - strand at 3261.579 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.125 mg/ml remove 3,258,277 + BPHYT_RS14255 0.54 +0.1 3,258,419 + BPHYT_RS14255 0.62 +1.0 3,258,501 + BPHYT_RS14255 0.66 +0.5 3,258,600 - BPHYT_RS14255 0.72 +1.0 3,258,625 - BPHYT_RS14255 0.73 +0.9 3,258,729 + BPHYT_RS14255 0.79 +0.4 3,259,060 - -0.0 3,259,233 + +0.2 3,259,563 + BPHYT_RS14260 0.23 +0.9 3,259,563 + BPHYT_RS14260 0.23 +1.1 3,259,564 - BPHYT_RS14260 0.23 +0.5 3,259,564 - BPHYT_RS14260 0.23 +0.2 3,259,564 - BPHYT_RS14260 0.23 +0.5 3,259,608 + BPHYT_RS14260 0.26 +0.2 3,259,656 + BPHYT_RS14260 0.29 +0.6 3,259,671 + BPHYT_RS14260 0.30 +0.3 3,259,671 + BPHYT_RS14260 0.30 +0.5 3,259,672 - BPHYT_RS14260 0.30 +0.2 3,259,672 - BPHYT_RS14260 0.30 +0.1 3,259,672 - BPHYT_RS14260 0.30 +0.1 3,259,672 - BPHYT_RS14260 0.30 -0.5 3,259,756 - BPHYT_RS14260 0.36 +0.2 3,260,224 + BPHYT_RS14260 0.68 +0.4 3,260,225 - BPHYT_RS14260 0.68 +0.6 3,260,295 + BPHYT_RS14260 0.73 +0.2 3,260,295 + BPHYT_RS14260 0.73 +0.5 3,260,295 + BPHYT_RS14260 0.73 +0.7 3,260,295 + BPHYT_RS14260 0.73 +0.3 3,260,397 + BPHYT_RS14260 0.80 +1.1 3,260,397 + BPHYT_RS14260 0.80 +0.4 3,260,397 + BPHYT_RS14260 0.80 +0.2 3,260,447 - BPHYT_RS14260 0.83 +0.2 3,260,460 + BPHYT_RS14260 0.84 +0.2 3,260,463 + BPHYT_RS14260 0.84 -0.1 3,260,463 + BPHYT_RS14260 0.84 +3.9 3,260,463 + BPHYT_RS14260 0.84 +0.4 3,260,464 - BPHYT_RS14260 0.84 +0.3 3,260,464 - BPHYT_RS14260 0.84 +0.2 3,260,464 - BPHYT_RS14260 0.84 -0.4 3,260,608 - +0.3 3,260,714 + +0.1 3,260,715 - +0.0 3,260,819 + +0.5 3,260,819 + +1.2 3,261,192 + BPHYT_RS14265 0.59 -0.7 3,261,578 + +0.3 3,261,578 + -1.5 3,261,579 - -0.2 3,261,579 - -0.2 3,261,579 - -0.0 3,261,579 - -0.1
Or see this region's nucleotide sequence