Experiment: Bacitracin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10810 and BPHYT_RS10815 are separated by 45 nucleotides BPHYT_RS10815 and BPHYT_RS10820 are separated by 3 nucleotides BPHYT_RS10820 and BPHYT_RS10825 overlap by 8 nucleotides BPHYT_RS10825 and BPHYT_RS10830 are separated by 23 nucleotides
BPHYT_RS10810: BPHYT_RS10810 - glucose-methanol-choline oxidoreductase, at 2,457,055 to 2,458,788
_RS10810
BPHYT_RS10815: BPHYT_RS10815 - crotonase, at 2,458,834 to 2,459,640
_RS10815
BPHYT_RS10820: BPHYT_RS10820 - 4-chlorobenzoate--CoA ligase, at 2,459,644 to 2,461,221
_RS10820
BPHYT_RS10825: BPHYT_RS10825 - (2Fe-2S)-binding protein, at 2,461,214 to 2,461,744
_RS10825
BPHYT_RS10830: BPHYT_RS10830 - carbon monoxide dehydrogenase, at 2,461,768 to 2,462,589
_RS10830
Position (kb)
2459
2460
2461
2462 Strain fitness (log2 ratio)
-1
0
1
2 at 2458.773 kb on - strand at 2458.804 kb on - strand at 2458.804 kb on - strand at 2458.919 kb on + strand, within BPHYT_RS10815 at 2458.919 kb on + strand, within BPHYT_RS10815 at 2459.756 kb on - strand at 2459.774 kb on + strand at 2460.017 kb on + strand, within BPHYT_RS10820 at 2460.018 kb on - strand, within BPHYT_RS10820 at 2460.288 kb on - strand, within BPHYT_RS10820 at 2460.306 kb on - strand, within BPHYT_RS10820 at 2460.409 kb on - strand, within BPHYT_RS10820 at 2460.449 kb on + strand, within BPHYT_RS10820 at 2460.593 kb on + strand, within BPHYT_RS10820 at 2460.725 kb on + strand, within BPHYT_RS10820 at 2460.725 kb on + strand, within BPHYT_RS10820 at 2461.134 kb on - strand at 2461.736 kb on + strand at 2461.737 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.125 mg/ml remove 2,458,773 - -0.0 2,458,804 - -0.2 2,458,804 - +0.1 2,458,919 + BPHYT_RS10815 0.11 +0.1 2,458,919 + BPHYT_RS10815 0.11 -0.9 2,459,756 - -0.6 2,459,774 + +0.1 2,460,017 + BPHYT_RS10820 0.24 -0.1 2,460,018 - BPHYT_RS10820 0.24 -0.6 2,460,288 - BPHYT_RS10820 0.41 +2.2 2,460,306 - BPHYT_RS10820 0.42 -0.1 2,460,409 - BPHYT_RS10820 0.48 -0.9 2,460,449 + BPHYT_RS10820 0.51 +0.6 2,460,593 + BPHYT_RS10820 0.60 -0.4 2,460,725 + BPHYT_RS10820 0.69 +0.3 2,460,725 + BPHYT_RS10820 0.69 -0.7 2,461,134 - +2.0 2,461,736 + -0.5 2,461,737 - -0.5
Or see this region's nucleotide sequence