Experiment: Paraquat dichloride 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0011 and SMa0013 overlap by 92 nucleotides SMa0013 and SMa0015 overlap by 4 nucleotides SMa0015 and SMa0017 are separated by 253 nucleotides
SMa0011: SMa0011 - selenocysteine synthase, at 7,074 to 8,474
SMa0011
SMa0013: SMa0013 - hypothetical protein, at 8,383 to 9,255
SMa0013
SMa0015: SMa0015 - selenocysteine-specific elongation factor, at 9,252 to 11,252
SMa0015
SMa0017: SMa0017 - hypothetical protein, at 11,506 to 12,063
SMa0017
Position (kb)
9
10
11
12 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 8.490 kb on + strand, within SMa0013 at 8.491 kb on - strand at 8.491 kb on - strand, within SMa0013 at 8.491 kb on - strand, within SMa0013 at 8.558 kb on - strand, within SMa0013 at 8.731 kb on + strand, within SMa0013 at 9.007 kb on + strand, within SMa0013 at 9.007 kb on + strand, within SMa0013 at 9.008 kb on - strand, within SMa0013 at 9.008 kb on - strand, within SMa0013 at 9.008 kb on - strand, within SMa0013 at 9.008 kb on - strand, within SMa0013 at 9.075 kb on + strand, within SMa0013 at 9.382 kb on - strand at 9.449 kb on + strand at 9.449 kb on + strand at 9.449 kb on + strand at 9.450 kb on - strand at 9.450 kb on - strand at 9.480 kb on + strand, within SMa0015 at 9.481 kb on - strand, within SMa0015 at 9.680 kb on - strand, within SMa0015 at 9.910 kb on + strand, within SMa0015 at 9.919 kb on + strand, within SMa0015 at 9.919 kb on + strand, within SMa0015 at 9.919 kb on + strand, within SMa0015 at 9.920 kb on - strand, within SMa0015 at 9.920 kb on - strand, within SMa0015 at 9.920 kb on - strand, within SMa0015 at 9.920 kb on - strand at 9.920 kb on - strand, within SMa0015 at 10.256 kb on + strand, within SMa0015 at 10.356 kb on + strand, within SMa0015 at 10.356 kb on + strand, within SMa0015 at 10.357 kb on - strand, within SMa0015 at 10.357 kb on - strand, within SMa0015 at 10.888 kb on + strand, within SMa0015 at 10.889 kb on - strand, within SMa0015 at 10.889 kb on - strand, within SMa0015 at 10.889 kb on - strand, within SMa0015 at 11.163 kb on + strand at 11.267 kb on - strand at 11.267 kb on - strand at 11.270 kb on + strand at 11.270 kb on + strand at 11.271 kb on - strand at 11.271 kb on - strand at 11.271 kb on - strand at 11.379 kb on + strand at 11.379 kb on + strand at 11.407 kb on - strand at 11.442 kb on + strand at 11.462 kb on - strand at 11.493 kb on + strand at 11.503 kb on + strand at 11.685 kb on + strand, within SMa0017 at 11.685 kb on + strand, within SMa0017 at 11.685 kb on + strand, within SMa0017 at 11.685 kb on + strand, within SMa0017 at 11.686 kb on - strand, within SMa0017 at 11.835 kb on + strand, within SMa0017 at 11.978 kb on + strand, within SMa0017 at 11.978 kb on + strand, within SMa0017 at 11.978 kb on + strand, within SMa0017 at 11.978 kb on + strand, within SMa0017 at 12.083 kb on - strand at 12.201 kb on + strand at 12.202 kb on - strand at 12.218 kb on - strand at 12.221 kb on + strand at 12.221 kb on + strand at 12.222 kb on - strand at 12.222 kb on - strand at 12.222 kb on - strand at 12.222 kb on - strand at 12.222 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.2 mg/ml remove 8,490 + SMa0013 0.12 +0.3 8,491 - +0.6 8,491 - SMa0013 0.12 -0.9 8,491 - SMa0013 0.12 -0.4 8,558 - SMa0013 0.20 -1.4 8,731 + SMa0013 0.40 -3.1 9,007 + SMa0013 0.71 +0.3 9,007 + SMa0013 0.71 -0.7 9,008 - SMa0013 0.72 -0.7 9,008 - SMa0013 0.72 -0.3 9,008 - SMa0013 0.72 -0.8 9,008 - SMa0013 0.72 +0.5 9,075 + SMa0013 0.79 -0.7 9,382 - +0.8 9,449 + -0.2 9,449 + -0.9 9,449 + +0.2 9,450 - -0.2 9,450 - -0.6 9,480 + SMa0015 0.11 -1.2 9,481 - SMa0015 0.11 +0.9 9,680 - SMa0015 0.21 -0.7 9,910 + SMa0015 0.33 -1.8 9,919 + SMa0015 0.33 -0.3 9,919 + SMa0015 0.33 -0.1 9,919 + SMa0015 0.33 -0.1 9,920 - SMa0015 0.33 -0.2 9,920 - SMa0015 0.33 -0.4 9,920 - SMa0015 0.33 -0.3 9,920 - +0.6 9,920 - SMa0015 0.33 -0.7 10,256 + SMa0015 0.50 -1.6 10,356 + SMa0015 0.55 -0.4 10,356 + SMa0015 0.55 +0.1 10,357 - SMa0015 0.55 -0.6 10,357 - SMa0015 0.55 -0.1 10,888 + SMa0015 0.82 -0.3 10,889 - SMa0015 0.82 -0.2 10,889 - SMa0015 0.82 -0.1 10,889 - SMa0015 0.82 -0.1 11,163 + -0.8 11,267 - +0.5 11,267 - -0.7 11,270 + +0.7 11,270 + +0.1 11,271 - +0.6 11,271 - +0.6 11,271 - +0.5 11,379 + +0.4 11,379 + +0.6 11,407 - -0.8 11,442 + +0.6 11,462 - +0.6 11,493 + -0.4 11,503 + +0.2 11,685 + SMa0017 0.32 +0.1 11,685 + SMa0017 0.32 +1.1 11,685 + SMa0017 0.32 +1.1 11,685 + SMa0017 0.32 +0.8 11,686 - SMa0017 0.32 +0.1 11,835 + SMa0017 0.59 +0.5 11,978 + SMa0017 0.85 -0.3 11,978 + SMa0017 0.85 +0.0 11,978 + SMa0017 0.85 +0.3 11,978 + SMa0017 0.85 -0.2 12,083 - +0.2 12,201 + -0.7 12,202 - +0.6 12,218 - -1.1 12,221 + -1.0 12,221 + +1.8 12,222 - -1.7 12,222 - -1.1 12,222 - -0.6 12,222 - -0.5 12,222 - +1.0
Or see this region's nucleotide sequence