Experiment: Paraquat dichloride 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20301 and SM_b20302 are separated by 147 nucleotides SM_b20302 and SM_b20303 are separated by 196 nucleotides SM_b20303 and SM_b20304 are separated by 56 nucleotides SM_b20304 and SM_b20306 overlap by 357 nucleotides SM_b20306 and SM_b20305 overlap by 73 nucleotides
SM_b20301: SM_b20301 - dehydgrogenase, at 308,842 to 309,972
_b20301
SM_b20302: SM_b20302 - hypothetical protein, at 310,120 to 310,281
_b20302
SM_b20303: SM_b20303 - hypothetical protein, at 310,478 to 310,705
_b20303
SM_b20304: SM_b20304 - ISRm2011-2 transposase, C-terminal portion, at 310,762 to 311,358
_b20304
SM_b20306: SM_b20306 - TRm2011-2 protein, at 311,002 to 311,373
_b20306
SM_b20305: SM_b20305 - ISRm2011-2 transposase, N-terminal portion, at 311,301 to 311,708
_b20305
Position (kb) 310
311 Strain fitness (log2 ratio) -2
-1
0
1 at 309.638 kb on + strand, within SM_b20301 at 309.639 kb on - strand, within SM_b20301 at 309.889 kb on - strand at 310.021 kb on + strand at 310.227 kb on - strand, within SM_b20302 at 310.237 kb on + strand, within SM_b20302 at 310.238 kb on - strand, within SM_b20302 at 310.238 kb on - strand, within SM_b20302 at 310.318 kb on - strand at 310.373 kb on - strand at 310.433 kb on + strand at 310.462 kb on + strand at 310.548 kb on - strand, within SM_b20303 at 310.548 kb on - strand, within SM_b20303 at 310.622 kb on + strand, within SM_b20303 at 310.623 kb on - strand, within SM_b20303 at 310.704 kb on - strand at 310.727 kb on - strand at 310.756 kb on - strand at 310.756 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.2 mg/ml remove 309,638 + SM_b20301 0.70 -0.3 309,639 - SM_b20301 0.70 -1.6 309,889 - +0.4 310,021 + -1.3 310,227 - SM_b20302 0.66 -0.6 310,237 + SM_b20302 0.72 +0.9 310,238 - SM_b20302 0.73 +0.7 310,238 - SM_b20302 0.73 +1.2 310,318 - +0.9 310,373 - -0.2 310,433 + -0.8 310,462 + +0.1 310,548 - SM_b20303 0.31 -0.1 310,548 - SM_b20303 0.31 -0.7 310,622 + SM_b20303 0.63 -1.0 310,623 - SM_b20303 0.64 -1.8 310,704 - +0.3 310,727 - -0.5 310,756 - -1.7 310,756 - +0.2
Or see this region's nucleotide sequence