Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS32000

Experiment: L-Histidine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS31990 and RR42_RS31995 are separated by 99 nucleotidesRR42_RS31995 and RR42_RS32000 are separated by 58 nucleotidesRR42_RS32000 and RR42_RS32005 are separated by 19 nucleotides RR42_RS31990: RR42_RS31990 - delta 4, 5-alpha steroid dehydrogenase, at 2,554,517 to 2,556,223 _RS31990 RR42_RS31995: RR42_RS31995 - flavin reductase, at 2,556,323 to 2,556,841 _RS31995 RR42_RS32000: RR42_RS32000 - 3-oxosteroid 1-dehydrogenase, at 2,556,900 to 2,558,642 _RS32000 RR42_RS32005: RR42_RS32005 - flavin-dependent monooxygenase, at 2,558,662 to 2,559,855 _RS32005 Position (kb) 2556 2557 2558 2559Strain fitness (log2 ratio) -3 -2 -1 0 1at 2555.974 kb on - strand, within RR42_RS31990at 2556.043 kb on - strand, within RR42_RS31990at 2556.378 kb on + strand, within RR42_RS31995at 2556.378 kb on + strand, within RR42_RS31995at 2556.545 kb on - strand, within RR42_RS31995at 2556.805 kb on + strandat 2556.806 kb on - strandat 2556.829 kb on + strandat 2556.830 kb on - strandat 2556.858 kb on - strandat 2556.858 kb on - strandat 2556.858 kb on - strandat 2556.858 kb on - strandat 2557.211 kb on - strand, within RR42_RS32000at 2557.211 kb on - strand, within RR42_RS32000at 2557.214 kb on + strand, within RR42_RS32000at 2557.215 kb on - strand, within RR42_RS32000at 2557.502 kb on - strand, within RR42_RS32000at 2557.526 kb on - strand, within RR42_RS32000at 2557.766 kb on - strand, within RR42_RS32000at 2557.998 kb on - strand, within RR42_RS32000at 2557.998 kb on - strand, within RR42_RS32000at 2558.291 kb on - strand, within RR42_RS32000at 2558.318 kb on - strand, within RR42_RS32000at 2558.348 kb on - strand, within RR42_RS32000at 2558.416 kb on + strand, within RR42_RS32000at 2558.417 kb on - strand, within RR42_RS32000at 2558.668 kb on - strandat 2558.741 kb on + strandat 2558.799 kb on - strand, within RR42_RS32005at 2558.799 kb on - strand, within RR42_RS32005at 2559.068 kb on + strand, within RR42_RS32005at 2559.068 kb on + strand, within RR42_RS32005at 2559.158 kb on + strand, within RR42_RS32005at 2559.318 kb on - strand, within RR42_RS32005at 2559.347 kb on + strand, within RR42_RS32005at 2559.489 kb on + strand, within RR42_RS32005at 2559.491 kb on + strand, within RR42_RS32005at 2559.492 kb on - strand, within RR42_RS32005at 2559.510 kb on + strand, within RR42_RS32005at 2559.593 kb on + strand, within RR42_RS32005at 2559.594 kb on - strand, within RR42_RS32005

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Histidine (C)
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2,555,974 - RR42_RS31990 0.85 -1.9
2,556,043 - RR42_RS31990 0.89 -0.7
2,556,378 + RR42_RS31995 0.11 +0.2
2,556,378 + RR42_RS31995 0.11 -0.1
2,556,545 - RR42_RS31995 0.43 +0.4
2,556,805 + -1.2
2,556,806 - +0.0
2,556,829 + +1.3
2,556,830 - +0.7
2,556,858 - -0.4
2,556,858 - -0.8
2,556,858 - -2.9
2,556,858 - -1.0
2,557,211 - RR42_RS32000 0.18 +0.2
2,557,211 - RR42_RS32000 0.18 -0.6
2,557,214 + RR42_RS32000 0.18 -1.0
2,557,215 - RR42_RS32000 0.18 -0.3
2,557,502 - RR42_RS32000 0.35 -0.6
2,557,526 - RR42_RS32000 0.36 +0.0
2,557,766 - RR42_RS32000 0.50 -0.8
2,557,998 - RR42_RS32000 0.63 -0.5
2,557,998 - RR42_RS32000 0.63 -2.0
2,558,291 - RR42_RS32000 0.80 -0.8
2,558,318 - RR42_RS32000 0.81 +0.4
2,558,348 - RR42_RS32000 0.83 -0.1
2,558,416 + RR42_RS32000 0.87 -0.2
2,558,417 - RR42_RS32000 0.87 -0.7
2,558,668 - -0.3
2,558,741 + -0.9
2,558,799 - RR42_RS32005 0.11 +0.6
2,558,799 - RR42_RS32005 0.11 -0.3
2,559,068 + RR42_RS32005 0.34 -0.6
2,559,068 + RR42_RS32005 0.34 -0.4
2,559,158 + RR42_RS32005 0.42 -1.0
2,559,318 - RR42_RS32005 0.55 +0.1
2,559,347 + RR42_RS32005 0.57 -0.7
2,559,489 + RR42_RS32005 0.69 +0.2
2,559,491 + RR42_RS32005 0.69 +1.0
2,559,492 - RR42_RS32005 0.70 -0.6
2,559,510 + RR42_RS32005 0.71 -2.4
2,559,593 + RR42_RS32005 0.78 -0.2
2,559,594 - RR42_RS32005 0.78 -0.2

Or see this region's nucleotide sequence