Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22130 and RR42_RS22135 are separated by 316 nucleotides RR42_RS22135 and RR42_RS22140 are separated by 1 nucleotides RR42_RS22140 and RR42_RS22145 overlap by 4 nucleotides
RR42_RS22130: RR42_RS22130 - amidase, at 305,211 to 306,653
_RS22130
RR42_RS22135: RR42_RS22135 - cytochrome C, at 306,970 to 307,455
_RS22135
RR42_RS22140: RR42_RS22140 - cytochrome C, at 307,457 to 307,912
_RS22140
RR42_RS22145: RR42_RS22145 - amine dehydrogenase, at 307,909 to 308,430
_RS22145
Position (kb) 306
307
308 Strain fitness (log2 ratio) -2
-1
0
1 at 306.029 kb on - strand, within RR42_RS22130 at 306.067 kb on + strand, within RR42_RS22130 at 306.101 kb on - strand, within RR42_RS22130 at 306.101 kb on - strand, within RR42_RS22130 at 306.583 kb on + strand at 306.583 kb on + strand at 307.331 kb on + strand, within RR42_RS22135 at 307.331 kb on + strand, within RR42_RS22135 at 307.332 kb on - strand, within RR42_RS22135 at 307.458 kb on + strand at 307.458 kb on + strand at 307.459 kb on - strand at 308.079 kb on - strand, within RR42_RS22145 at 308.090 kb on + strand, within RR42_RS22145 at 308.216 kb on + strand, within RR42_RS22145 at 308.216 kb on + strand, within RR42_RS22145 at 308.217 kb on - strand, within RR42_RS22145
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 306,029 - RR42_RS22130 0.57 +0.8 306,067 + RR42_RS22130 0.59 -0.4 306,101 - RR42_RS22130 0.62 +0.5 306,101 - RR42_RS22130 0.62 +0.2 306,583 + +0.5 306,583 + +0.4 307,331 + RR42_RS22135 0.74 -0.6 307,331 + RR42_RS22135 0.74 -0.5 307,332 - RR42_RS22135 0.74 -1.9 307,458 + +0.9 307,458 + +1.0 307,459 - +0.8 308,079 - RR42_RS22145 0.33 +0.1 308,090 + RR42_RS22145 0.35 -1.0 308,216 + RR42_RS22145 0.59 +0.1 308,216 + RR42_RS22145 0.59 -0.2 308,217 - RR42_RS22145 0.59 +0.7
Or see this region's nucleotide sequence