Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15115 and RR42_RS15120 are separated by 108 nucleotides RR42_RS15120 and RR42_RS15125 are separated by 96 nucleotides RR42_RS15125 and RR42_RS15130 are separated by 131 nucleotides
RR42_RS15115: RR42_RS15115 - threonine aldolase, at 3,271,209 to 3,272,240
_RS15115
RR42_RS15120: RR42_RS15120 - 4-oxalocrotonate tautomerase, at 3,272,349 to 3,272,537
_RS15120
RR42_RS15125: RR42_RS15125 - aldolase, at 3,272,634 to 3,273,407
_RS15125
RR42_RS15130: RR42_RS15130 - MFS transporter, at 3,273,539 to 3,274,750
_RS15130
Position (kb)
3272
3273
3274 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3271.795 kb on + strand, within RR42_RS15115 at 3271.982 kb on + strand, within RR42_RS15115 at 3271.983 kb on - strand, within RR42_RS15115 at 3271.983 kb on - strand, within RR42_RS15115 at 3272.015 kb on - strand, within RR42_RS15115 at 3272.147 kb on - strand at 3272.215 kb on + strand at 3272.215 kb on + strand at 3272.216 kb on - strand at 3272.350 kb on + strand at 3272.350 kb on + strand at 3272.351 kb on - strand at 3272.351 kb on - strand at 3272.351 kb on - strand at 3272.417 kb on + strand, within RR42_RS15120 at 3272.520 kb on + strand at 3272.520 kb on + strand at 3272.521 kb on - strand at 3272.521 kb on - strand at 3272.521 kb on - strand at 3272.527 kb on + strand at 3272.661 kb on - strand at 3272.833 kb on + strand, within RR42_RS15125 at 3272.834 kb on - strand, within RR42_RS15125 at 3272.834 kb on - strand, within RR42_RS15125 at 3272.834 kb on - strand, within RR42_RS15125 at 3272.956 kb on + strand, within RR42_RS15125 at 3272.956 kb on + strand, within RR42_RS15125 at 3272.957 kb on - strand, within RR42_RS15125 at 3272.965 kb on + strand at 3272.980 kb on + strand, within RR42_RS15125 at 3273.054 kb on - strand, within RR42_RS15125 at 3273.122 kb on + strand, within RR42_RS15125 at 3273.266 kb on + strand, within RR42_RS15125 at 3273.266 kb on + strand, within RR42_RS15125 at 3273.266 kb on + strand, within RR42_RS15125 at 3273.266 kb on + strand, within RR42_RS15125 at 3273.266 kb on + strand, within RR42_RS15125 at 3273.267 kb on - strand, within RR42_RS15125 at 3273.274 kb on + strand, within RR42_RS15125 at 3273.274 kb on + strand, within RR42_RS15125 at 3273.274 kb on + strand, within RR42_RS15125 at 3273.274 kb on + strand, within RR42_RS15125 at 3273.275 kb on - strand, within RR42_RS15125 at 3273.275 kb on - strand, within RR42_RS15125 at 3273.480 kb on - strand at 3273.485 kb on - strand at 3273.485 kb on - strand at 3273.540 kb on + strand at 3273.541 kb on - strand at 3273.687 kb on + strand, within RR42_RS15130 at 3273.688 kb on - strand, within RR42_RS15130 at 3273.867 kb on + strand, within RR42_RS15130 at 3273.867 kb on + strand, within RR42_RS15130 at 3273.868 kb on - strand, within RR42_RS15130 at 3273.868 kb on - strand, within RR42_RS15130 at 3273.868 kb on - strand, within RR42_RS15130 at 3273.868 kb on - strand, within RR42_RS15130 at 3273.871 kb on + strand, within RR42_RS15130 at 3273.871 kb on + strand, within RR42_RS15130 at 3273.871 kb on + strand, within RR42_RS15130 at 3273.871 kb on + strand, within RR42_RS15130 at 3273.871 kb on + strand, within RR42_RS15130
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 3,271,795 + RR42_RS15115 0.57 -0.5 3,271,982 + RR42_RS15115 0.75 -0.0 3,271,983 - RR42_RS15115 0.75 +1.4 3,271,983 - RR42_RS15115 0.75 +2.1 3,272,015 - RR42_RS15115 0.78 -0.0 3,272,147 - +0.0 3,272,215 + +1.0 3,272,215 + -0.9 3,272,216 - -0.3 3,272,350 + -0.7 3,272,350 + +1.0 3,272,351 - +1.5 3,272,351 - +0.3 3,272,351 - -0.4 3,272,417 + RR42_RS15120 0.36 -1.0 3,272,520 + +0.6 3,272,520 + +0.2 3,272,521 - -0.9 3,272,521 - +0.2 3,272,521 - +0.4 3,272,527 + -1.3 3,272,661 - +0.2 3,272,833 + RR42_RS15125 0.26 +1.5 3,272,834 - RR42_RS15125 0.26 +0.6 3,272,834 - RR42_RS15125 0.26 -1.2 3,272,834 - RR42_RS15125 0.26 +0.7 3,272,956 + RR42_RS15125 0.42 -0.8 3,272,956 + RR42_RS15125 0.42 +0.6 3,272,957 - RR42_RS15125 0.42 -0.6 3,272,965 + +0.4 3,272,980 + RR42_RS15125 0.45 +2.0 3,273,054 - RR42_RS15125 0.54 +0.4 3,273,122 + RR42_RS15125 0.63 +0.1 3,273,266 + RR42_RS15125 0.82 +0.3 3,273,266 + RR42_RS15125 0.82 -0.1 3,273,266 + RR42_RS15125 0.82 -0.1 3,273,266 + RR42_RS15125 0.82 +0.5 3,273,266 + RR42_RS15125 0.82 -0.6 3,273,267 - RR42_RS15125 0.82 +1.2 3,273,274 + RR42_RS15125 0.83 +0.3 3,273,274 + RR42_RS15125 0.83 -2.1 3,273,274 + RR42_RS15125 0.83 -3.0 3,273,274 + RR42_RS15125 0.83 +0.3 3,273,275 - RR42_RS15125 0.83 -3.1 3,273,275 - RR42_RS15125 0.83 -1.2 3,273,480 - +1.3 3,273,485 - -1.1 3,273,485 - +0.7 3,273,540 + +0.3 3,273,541 - +1.7 3,273,687 + RR42_RS15130 0.12 +0.0 3,273,688 - RR42_RS15130 0.12 +0.8 3,273,867 + RR42_RS15130 0.27 +0.2 3,273,867 + RR42_RS15130 0.27 -0.9 3,273,868 - RR42_RS15130 0.27 +1.0 3,273,868 - RR42_RS15130 0.27 -1.1 3,273,868 - RR42_RS15130 0.27 -0.9 3,273,868 - RR42_RS15130 0.27 -0.7 3,273,871 + RR42_RS15130 0.27 +2.0 3,273,871 + RR42_RS15130 0.27 +0.1 3,273,871 + RR42_RS15130 0.27 -0.7 3,273,871 + RR42_RS15130 0.27 -0.3 3,273,871 + RR42_RS15130 0.27 +0.2
Or see this region's nucleotide sequence