Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06665 and RR42_RS06670 are separated by 220 nucleotides RR42_RS06670 and RR42_RS06675 are separated by 17 nucleotides RR42_RS06675 and RR42_RS06680 are separated by 60 nucleotides RR42_RS06680 and RR42_RS06685 are separated by 108 nucleotides RR42_RS06685 and RR42_RS06690 overlap by 4 nucleotides
RR42_RS06665: RR42_RS06665 - reverse transcriptase, at 1,451,819 to 1,452,994
_RS06665
RR42_RS06670: RR42_RS06670 - hypothetical protein, at 1,453,215 to 1,453,568
_RS06670
RR42_RS06675: RR42_RS06675 - hypothetical protein, at 1,453,586 to 1,454,197
_RS06675
RR42_RS06680: RR42_RS06680 - hypothetical protein, at 1,454,258 to 1,454,866
_RS06680
RR42_RS06685: RR42_RS06685 - hypothetical protein, at 1,454,975 to 1,455,172
_RS06685
RR42_RS06690: RR42_RS06690 - hypothetical protein, at 1,455,169 to 1,455,624
_RS06690
Position (kb)
1453
1454
1455 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1452.631 kb on + strand, within RR42_RS06665 at 1452.632 kb on - strand, within RR42_RS06665 at 1452.651 kb on + strand, within RR42_RS06665 at 1452.651 kb on + strand, within RR42_RS06665 at 1452.652 kb on - strand, within RR42_RS06665 at 1452.652 kb on - strand, within RR42_RS06665 at 1452.675 kb on + strand, within RR42_RS06665 at 1452.675 kb on + strand, within RR42_RS06665 at 1452.675 kb on + strand, within RR42_RS06665 at 1452.675 kb on + strand, within RR42_RS06665 at 1452.676 kb on - strand, within RR42_RS06665 at 1452.676 kb on - strand, within RR42_RS06665 at 1452.680 kb on + strand, within RR42_RS06665 at 1452.681 kb on - strand, within RR42_RS06665 at 1452.681 kb on - strand, within RR42_RS06665 at 1452.719 kb on + strand, within RR42_RS06665 at 1452.809 kb on + strand, within RR42_RS06665 at 1452.810 kb on - strand, within RR42_RS06665 at 1452.821 kb on + strand, within RR42_RS06665 at 1452.883 kb on - strand at 1452.966 kb on - strand at 1453.046 kb on - strand at 1453.284 kb on + strand, within RR42_RS06670 at 1453.490 kb on + strand, within RR42_RS06670 at 1453.490 kb on + strand, within RR42_RS06670 at 1453.528 kb on + strand, within RR42_RS06670 at 1453.528 kb on + strand, within RR42_RS06670 at 1453.541 kb on - strand at 1453.580 kb on - strand at 1453.583 kb on + strand at 1453.621 kb on - strand at 1453.752 kb on + strand, within RR42_RS06675 at 1453.752 kb on + strand, within RR42_RS06675 at 1453.785 kb on + strand, within RR42_RS06675 at 1454.019 kb on + strand, within RR42_RS06675 at 1454.019 kb on + strand, within RR42_RS06675 at 1454.042 kb on + strand, within RR42_RS06675 at 1454.043 kb on - strand, within RR42_RS06675 at 1454.043 kb on - strand, within RR42_RS06675 at 1454.043 kb on - strand, within RR42_RS06675 at 1454.100 kb on - strand, within RR42_RS06675 at 1454.218 kb on - strand at 1454.221 kb on + strand at 1454.222 kb on - strand at 1454.222 kb on - strand at 1454.392 kb on - strand, within RR42_RS06680 at 1454.467 kb on + strand, within RR42_RS06680 at 1454.469 kb on + strand, within RR42_RS06680 at 1454.469 kb on + strand, within RR42_RS06680 at 1454.469 kb on + strand, within RR42_RS06680 at 1454.469 kb on + strand, within RR42_RS06680 at 1454.475 kb on + strand, within RR42_RS06680 at 1454.553 kb on + strand, within RR42_RS06680 at 1454.586 kb on + strand, within RR42_RS06680 at 1454.587 kb on - strand, within RR42_RS06680 at 1454.750 kb on - strand, within RR42_RS06680 at 1454.776 kb on - strand, within RR42_RS06680 at 1454.930 kb on + strand at 1454.930 kb on + strand at 1454.931 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 1,452,631 + RR42_RS06665 0.69 -0.3 1,452,632 - RR42_RS06665 0.69 -0.0 1,452,651 + RR42_RS06665 0.71 +0.2 1,452,651 + RR42_RS06665 0.71 +0.0 1,452,652 - RR42_RS06665 0.71 +0.5 1,452,652 - RR42_RS06665 0.71 +1.5 1,452,675 + RR42_RS06665 0.73 -0.1 1,452,675 + RR42_RS06665 0.73 -0.2 1,452,675 + RR42_RS06665 0.73 -2.9 1,452,675 + RR42_RS06665 0.73 +2.7 1,452,676 - RR42_RS06665 0.73 +0.5 1,452,676 - RR42_RS06665 0.73 +0.1 1,452,680 + RR42_RS06665 0.73 +0.2 1,452,681 - RR42_RS06665 0.73 +0.5 1,452,681 - RR42_RS06665 0.73 -0.0 1,452,719 + RR42_RS06665 0.77 -2.4 1,452,809 + RR42_RS06665 0.84 -1.7 1,452,810 - RR42_RS06665 0.84 +0.1 1,452,821 + RR42_RS06665 0.85 -0.2 1,452,883 - +1.6 1,452,966 - -0.7 1,453,046 - -0.1 1,453,284 + RR42_RS06670 0.19 +0.9 1,453,490 + RR42_RS06670 0.78 -0.1 1,453,490 + RR42_RS06670 0.78 +0.4 1,453,528 + RR42_RS06670 0.88 -0.5 1,453,528 + RR42_RS06670 0.88 -1.7 1,453,541 - +1.3 1,453,580 - -1.6 1,453,583 + -0.4 1,453,621 - +1.0 1,453,752 + RR42_RS06675 0.27 -0.5 1,453,752 + RR42_RS06675 0.27 +0.0 1,453,785 + RR42_RS06675 0.33 +0.1 1,454,019 + RR42_RS06675 0.71 +1.5 1,454,019 + RR42_RS06675 0.71 -2.2 1,454,042 + RR42_RS06675 0.75 +0.2 1,454,043 - RR42_RS06675 0.75 +0.6 1,454,043 - RR42_RS06675 0.75 +1.5 1,454,043 - RR42_RS06675 0.75 +0.8 1,454,100 - RR42_RS06675 0.84 +1.2 1,454,218 - -0.3 1,454,221 + +1.0 1,454,222 - +1.1 1,454,222 - +0.9 1,454,392 - RR42_RS06680 0.22 +0.5 1,454,467 + RR42_RS06680 0.34 -0.2 1,454,469 + RR42_RS06680 0.35 +1.1 1,454,469 + RR42_RS06680 0.35 +0.0 1,454,469 + RR42_RS06680 0.35 +0.9 1,454,469 + RR42_RS06680 0.35 +0.7 1,454,475 + RR42_RS06680 0.36 -0.4 1,454,553 + RR42_RS06680 0.48 +0.2 1,454,586 + RR42_RS06680 0.54 +0.3 1,454,587 - RR42_RS06680 0.54 +0.6 1,454,750 - RR42_RS06680 0.81 -2.1 1,454,776 - RR42_RS06680 0.85 -0.9 1,454,930 + -1.3 1,454,930 + +0.7 1,454,931 - +0.6
Or see this region's nucleotide sequence