Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05075 and RR42_RS05080 overlap by 4 nucleotides RR42_RS05080 and RR42_RS05085 are separated by 57 nucleotides RR42_RS05085 and RR42_RS05095 are separated by 850 nucleotides
RR42_RS05075: RR42_RS05075 - aminocarboxymuconate-semialdehyde decarboxylase, at 1,121,834 to 1,122,847
_RS05075
RR42_RS05080: RR42_RS05080 - 3-hydroxyanthranilate 3,4-dioxygenase, at 1,122,844 to 1,123,404
_RS05080
RR42_RS05085: RR42_RS05085 - 2-aminomuconate deaminase, at 1,123,462 to 1,123,884
_RS05085
RR42_RS05095: RR42_RS05095 - 4-hyroxy-2-oxovalerate aldolase, at 1,124,735 to 1,125,739
_RS05095
Position (kb)
1123
1124 Strain fitness (log2 ratio)
-2
-1
0
1 at 1122.688 kb on - strand, within RR42_RS05075 at 1122.945 kb on - strand, within RR42_RS05080 at 1122.945 kb on - strand, within RR42_RS05080 at 1123.034 kb on + strand, within RR42_RS05080 at 1123.227 kb on - strand, within RR42_RS05080 at 1123.227 kb on - strand, within RR42_RS05080 at 1123.330 kb on + strand, within RR42_RS05080 at 1123.331 kb on - strand, within RR42_RS05080 at 1123.395 kb on - strand at 1123.572 kb on - strand, within RR42_RS05085 at 1123.581 kb on - strand, within RR42_RS05085 at 1123.592 kb on + strand, within RR42_RS05085 at 1123.878 kb on + strand at 1123.907 kb on + strand at 1123.907 kb on + strand at 1124.559 kb on + strand at 1124.559 kb on + strand at 1124.642 kb on - strand at 1124.883 kb on + strand, within RR42_RS05095
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 1,122,688 - RR42_RS05075 0.84 -0.1 1,122,945 - RR42_RS05080 0.18 -1.6 1,122,945 - RR42_RS05080 0.18 -0.5 1,123,034 + RR42_RS05080 0.34 +0.5 1,123,227 - RR42_RS05080 0.68 -0.2 1,123,227 - RR42_RS05080 0.68 -1.3 1,123,330 + RR42_RS05080 0.87 +0.7 1,123,331 - RR42_RS05080 0.87 +0.6 1,123,395 - -1.8 1,123,572 - RR42_RS05085 0.26 -0.9 1,123,581 - RR42_RS05085 0.28 -1.4 1,123,592 + RR42_RS05085 0.31 -0.5 1,123,878 + -0.3 1,123,907 + +1.2 1,123,907 + +1.0 1,124,559 + -0.6 1,124,559 + -0.0 1,124,642 - -0.9 1,124,883 + RR42_RS05095 0.15 +0.4
Or see this region's nucleotide sequence